Functional Analysis of DNMT1 SNPs (rs2228611 and rs2114724) Associated with Schizophrenia
A recent study showed the association of minor alleles of rs2228611 (T allele) and rs2114724 (T allele) of DNMT1 with schizophrenia (SZ) and suggested their effects on splicing of the transcripts. We performed a replication study using 310 controls and 304 SZ patients and confirmed the association o...
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2021-01-01
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| Series: | Genetics Research |
| Online Access: | http://dx.doi.org/10.1155/2021/6698979 |
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| author | Sonal Saxena Pranay Amruth Maroju Sumana Choudhury Vidhya Chitta Voina Poonam Naik Kavitha Gowdhaman Poornima Kkani Kiranmai Chennoju S. Ganesh Kumar C. Ramasubramanian G. Prasad Rao Trinath Jamma Kumar Pranav Narayan K. Naga Mohan |
| author_facet | Sonal Saxena Pranay Amruth Maroju Sumana Choudhury Vidhya Chitta Voina Poonam Naik Kavitha Gowdhaman Poornima Kkani Kiranmai Chennoju S. Ganesh Kumar C. Ramasubramanian G. Prasad Rao Trinath Jamma Kumar Pranav Narayan K. Naga Mohan |
| author_sort | Sonal Saxena |
| collection | DOAJ |
| description | A recent study showed the association of minor alleles of rs2228611 (T allele) and rs2114724 (T allele) of DNMT1 with schizophrenia (SZ) and suggested their effects on splicing of the transcripts. We performed a replication study using 310 controls and 304 SZ patients and confirmed the association of the homozygous minor allele genotypes with SZ (P = 0.04 for rs2114724 and P = 0.007 for rs2228611). This significant association persisted after Bonferroni correction when the previously published data of 301 controls and 325 patients were also considered (P ≤ 0.0002). In addition, we found that the proportion of male patients with homozygous minor alleles at rs2114724 was significantly higher than that of females (P = 0.002). When haplotype analysis of both loci was performed, we observed a significant association of T/T–T/T and T/T–C/T (P = 0.04) haplotypes with SZ. To gain insights into the functional effects of the two SNPs on the levels of DNMT1 transcripts, quantitative real-time PCR experiments were performed using peripheral blood monocytes from 10 individuals each with T/T–T/T (homozygous minor allele), C/T–C/T (heterozygous), and C/C–C/C (homozygous major allele) haplotypes. Independently, the levels of DNMT1 protein were also compared in three individuals each by immunofluorescence. These results suggest that neither DNMT1 transcript nor the protein levels were significantly different in the peripheral blood monocytes among the individuals studied for the three groups. Taken together, our results confirm that the two minor alleles in homozygosity are associated with SZ but with no discernible effects on transcript or protein levels of DNMT1 in the peripheral blood monocytes of the small number of samples tested. |
| format | Article |
| id | doaj-art-28798b94df534441895eee949185e1f1 |
| institution | Kabale University |
| issn | 0016-6723 1469-5073 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | Genetics Research |
| spelling | doaj-art-28798b94df534441895eee949185e1f12025-02-03T01:01:55ZengWileyGenetics Research0016-67231469-50732021-01-01202110.1155/2021/66989796698979Functional Analysis of DNMT1 SNPs (rs2228611 and rs2114724) Associated with SchizophreniaSonal Saxena0Pranay Amruth Maroju1Sumana Choudhury2Vidhya Chitta Voina3Poonam Naik4Kavitha Gowdhaman5Poornima Kkani6Kiranmai Chennoju7S. Ganesh Kumar8C. Ramasubramanian9G. Prasad Rao10Trinath Jamma11Kumar Pranav Narayan12K. Naga Mohan13Department of Biological Sciences, Birla Institute of Technology and Science, Pilani – Hyderabad Campus, Hyderabad, IndiaDepartment of Biological Sciences, Birla Institute of Technology and Science, Pilani – Hyderabad Campus, Hyderabad, IndiaDepartment of Biological Sciences, Birla Institute of Technology and Science, Pilani – Hyderabad Campus, Hyderabad, IndiaDepartment of Biological Sciences, Birla Institute of Technology and Science, Pilani – Hyderabad Campus, Hyderabad, IndiaDepartment of Biological Sciences, Birla Institute of Technology and Science, Pilani – Hyderabad Campus, Hyderabad, IndiaDepartment of Biological Sciences, Birla Institute of Technology and Science, Pilani – Hyderabad Campus, Hyderabad, IndiaDepartment of Zoology, Thiagarajar College, Madurai, IndiaDepartment of Biological Sciences, Birla Institute of Technology and Science, Pilani – Hyderabad Campus, Hyderabad, IndiaDepartment of Psychiatry, M.S. Chellamuthu Trust and Research Foundation, Madurai, IndiaDepartment of Psychiatry, M.S. Chellamuthu Trust and Research Foundation, Madurai, IndiaAsha Hospital Institute of Medical Psychology, Hyderabad 500034, IndiaDepartment of Biological Sciences, Birla Institute of Technology and Science, Pilani – Hyderabad Campus, Hyderabad, IndiaDepartment of Biological Sciences, Birla Institute of Technology and Science, Pilani – Hyderabad Campus, Hyderabad, IndiaDepartment of Biological Sciences, Birla Institute of Technology and Science, Pilani – Hyderabad Campus, Hyderabad, IndiaA recent study showed the association of minor alleles of rs2228611 (T allele) and rs2114724 (T allele) of DNMT1 with schizophrenia (SZ) and suggested their effects on splicing of the transcripts. We performed a replication study using 310 controls and 304 SZ patients and confirmed the association of the homozygous minor allele genotypes with SZ (P = 0.04 for rs2114724 and P = 0.007 for rs2228611). This significant association persisted after Bonferroni correction when the previously published data of 301 controls and 325 patients were also considered (P ≤ 0.0002). In addition, we found that the proportion of male patients with homozygous minor alleles at rs2114724 was significantly higher than that of females (P = 0.002). When haplotype analysis of both loci was performed, we observed a significant association of T/T–T/T and T/T–C/T (P = 0.04) haplotypes with SZ. To gain insights into the functional effects of the two SNPs on the levels of DNMT1 transcripts, quantitative real-time PCR experiments were performed using peripheral blood monocytes from 10 individuals each with T/T–T/T (homozygous minor allele), C/T–C/T (heterozygous), and C/C–C/C (homozygous major allele) haplotypes. Independently, the levels of DNMT1 protein were also compared in three individuals each by immunofluorescence. These results suggest that neither DNMT1 transcript nor the protein levels were significantly different in the peripheral blood monocytes among the individuals studied for the three groups. Taken together, our results confirm that the two minor alleles in homozygosity are associated with SZ but with no discernible effects on transcript or protein levels of DNMT1 in the peripheral blood monocytes of the small number of samples tested.http://dx.doi.org/10.1155/2021/6698979 |
| spellingShingle | Sonal Saxena Pranay Amruth Maroju Sumana Choudhury Vidhya Chitta Voina Poonam Naik Kavitha Gowdhaman Poornima Kkani Kiranmai Chennoju S. Ganesh Kumar C. Ramasubramanian G. Prasad Rao Trinath Jamma Kumar Pranav Narayan K. Naga Mohan Functional Analysis of DNMT1 SNPs (rs2228611 and rs2114724) Associated with Schizophrenia Genetics Research |
| title | Functional Analysis of DNMT1 SNPs (rs2228611 and rs2114724) Associated with Schizophrenia |
| title_full | Functional Analysis of DNMT1 SNPs (rs2228611 and rs2114724) Associated with Schizophrenia |
| title_fullStr | Functional Analysis of DNMT1 SNPs (rs2228611 and rs2114724) Associated with Schizophrenia |
| title_full_unstemmed | Functional Analysis of DNMT1 SNPs (rs2228611 and rs2114724) Associated with Schizophrenia |
| title_short | Functional Analysis of DNMT1 SNPs (rs2228611 and rs2114724) Associated with Schizophrenia |
| title_sort | functional analysis of dnmt1 snps rs2228611 and rs2114724 associated with schizophrenia |
| url | http://dx.doi.org/10.1155/2021/6698979 |
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