Development and evaluation of a chicken embryo fibroblast cell culture based live attenuated Indian strain duck plague vaccine
Duck plague (DP) is an acute, contagious and fatal disease, caused by duck enteritis virus (DEV), with worldwide distribution causing several outbreaks and posing severe economic losses. The present study was carried out with a goal of development of a live attenuated cell culture based DP vaccine u...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Veterinary Quarterly |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/01652176.2024.2350668 |
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| author | Satyabrata Dandapat Suresh Bindu Gaurav Kumar Sharma Sivasankar Panickan Sukdeb Nandi G. Saikumar Kuldeep Dhama |
| author_facet | Satyabrata Dandapat Suresh Bindu Gaurav Kumar Sharma Sivasankar Panickan Sukdeb Nandi G. Saikumar Kuldeep Dhama |
| author_sort | Satyabrata Dandapat |
| collection | DOAJ |
| description | Duck plague (DP) is an acute, contagious and fatal disease, caused by duck enteritis virus (DEV), with worldwide distribution causing several outbreaks and posing severe economic losses. The present study was carried out with a goal of development of a live attenuated cell culture based DP vaccine using an Indian strain of DEV and evaluation of its safety, efficacy along with complete genome analysis. The live attenuated DP vaccine (DPvac/IVRI-19) was developed by serial propagation of a virulent isolate of DEV (DEV/India/IVRI-2016) in the chicken embryo fibroblast (CEF) primary cell culture. Adaptation of DEV in CEF cell culture was indicated by more rapid appearance of cytopathic effects (CPE) and gradual increase of virus titre, which reached up to 107.5 TCID50/mL after 41 passages. The safety, immunogenicity and efficacy of the vaccine were determined by immunization trials in ducklings. The DPvac/IVRI-19 was found to be avirulent and completely safe in the ducklings. Further, the vaccine induced both humoral and cell mediated immune responses and afforded 100% protection against the virulent DEV challenge. A comparison of the whole genome of DPvac/IVRI-19 (MZ911871) and DEV/India/IVRI-2016 (MZ824102) revealed significant number of mutations, which might be associated with viral attenuation. Phylogenetic tree of DEV/India/IVRI-2016 revealed its evolutionary relationship with other DEV isolates, but it formed a separate cluster with certain unique mutations. Thus, with the proven safety and 100% efficacy, the DPvac/IVRI-19 is suitable for large scale production with precisely pure form of vaccine and has potential utility at national and global levels. |
| format | Article |
| id | doaj-art-286fcd8a469f4b21adcb12f387bac3e7 |
| institution | Kabale University |
| issn | 0165-2176 1875-5941 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Veterinary Quarterly |
| spelling | doaj-art-286fcd8a469f4b21adcb12f387bac3e72024-12-03T17:25:19ZengTaylor & Francis GroupVeterinary Quarterly0165-21761875-59412024-12-0144111210.1080/01652176.2024.2350668Development and evaluation of a chicken embryo fibroblast cell culture based live attenuated Indian strain duck plague vaccineSatyabrata Dandapat0Suresh Bindu1Gaurav Kumar Sharma2Sivasankar Panickan3Sukdeb Nandi4G. Saikumar5Kuldeep Dhama6Immunology Section, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, IndiaImmunology Section, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, IndiaCenter for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, IndiaImmunology Section, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, IndiaCenter for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, IndiaDivision of Pathology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, IndiaDivision of Pathology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, IndiaDuck plague (DP) is an acute, contagious and fatal disease, caused by duck enteritis virus (DEV), with worldwide distribution causing several outbreaks and posing severe economic losses. The present study was carried out with a goal of development of a live attenuated cell culture based DP vaccine using an Indian strain of DEV and evaluation of its safety, efficacy along with complete genome analysis. The live attenuated DP vaccine (DPvac/IVRI-19) was developed by serial propagation of a virulent isolate of DEV (DEV/India/IVRI-2016) in the chicken embryo fibroblast (CEF) primary cell culture. Adaptation of DEV in CEF cell culture was indicated by more rapid appearance of cytopathic effects (CPE) and gradual increase of virus titre, which reached up to 107.5 TCID50/mL after 41 passages. The safety, immunogenicity and efficacy of the vaccine were determined by immunization trials in ducklings. The DPvac/IVRI-19 was found to be avirulent and completely safe in the ducklings. Further, the vaccine induced both humoral and cell mediated immune responses and afforded 100% protection against the virulent DEV challenge. A comparison of the whole genome of DPvac/IVRI-19 (MZ911871) and DEV/India/IVRI-2016 (MZ824102) revealed significant number of mutations, which might be associated with viral attenuation. Phylogenetic tree of DEV/India/IVRI-2016 revealed its evolutionary relationship with other DEV isolates, but it formed a separate cluster with certain unique mutations. Thus, with the proven safety and 100% efficacy, the DPvac/IVRI-19 is suitable for large scale production with precisely pure form of vaccine and has potential utility at national and global levels.https://www.tandfonline.com/doi/10.1080/01652176.2024.2350668Duck enteritis virusduck plaguechicken embryo fibroblastcell culture attenuated vaccinesafetyefficacy |
| spellingShingle | Satyabrata Dandapat Suresh Bindu Gaurav Kumar Sharma Sivasankar Panickan Sukdeb Nandi G. Saikumar Kuldeep Dhama Development and evaluation of a chicken embryo fibroblast cell culture based live attenuated Indian strain duck plague vaccine Veterinary Quarterly Duck enteritis virus duck plague chicken embryo fibroblast cell culture attenuated vaccine safety efficacy |
| title | Development and evaluation of a chicken embryo fibroblast cell culture based live attenuated Indian strain duck plague vaccine |
| title_full | Development and evaluation of a chicken embryo fibroblast cell culture based live attenuated Indian strain duck plague vaccine |
| title_fullStr | Development and evaluation of a chicken embryo fibroblast cell culture based live attenuated Indian strain duck plague vaccine |
| title_full_unstemmed | Development and evaluation of a chicken embryo fibroblast cell culture based live attenuated Indian strain duck plague vaccine |
| title_short | Development and evaluation of a chicken embryo fibroblast cell culture based live attenuated Indian strain duck plague vaccine |
| title_sort | development and evaluation of a chicken embryo fibroblast cell culture based live attenuated indian strain duck plague vaccine |
| topic | Duck enteritis virus duck plague chicken embryo fibroblast cell culture attenuated vaccine safety efficacy |
| url | https://www.tandfonline.com/doi/10.1080/01652176.2024.2350668 |
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