Biological Behavior of Bioactive Glasses SinGlass (45S5) and SinGlass High (F18) in the Repair of Critical Bone Defects
This study evaluated the osteogenic potential of the bioactive glasses SinGlass (45S5) and SinGlass High (F18) in regenerating critical bone defects in rat calvaria. Both biomaterials promoted new bone formation around the particles, with the SinGlass High (F18) group exhibiting a higher rate of bon...
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MDPI AG
2025-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/15/1/112 |
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| author | Dayane Maria Braz Nogueira Marcelie Priscila de Oliveira Rosso Paulo Sérgio da Silva Santos Manoel Damião Sousa-Neto Alice Corrêa Silva-Sousa Cleverson Teixeira Soares Carlos Henrique Bertoni Reis Jéssica de Oliveira Rossi Cleuber Rodrigo de Souza Bueno Daniela Vieira Buchaim Rogério Leone Buchaim Mariana Schutzer Ragghianti Zangrando |
| author_facet | Dayane Maria Braz Nogueira Marcelie Priscila de Oliveira Rosso Paulo Sérgio da Silva Santos Manoel Damião Sousa-Neto Alice Corrêa Silva-Sousa Cleverson Teixeira Soares Carlos Henrique Bertoni Reis Jéssica de Oliveira Rossi Cleuber Rodrigo de Souza Bueno Daniela Vieira Buchaim Rogério Leone Buchaim Mariana Schutzer Ragghianti Zangrando |
| author_sort | Dayane Maria Braz Nogueira |
| collection | DOAJ |
| description | This study evaluated the osteogenic potential of the bioactive glasses SinGlass (45S5) and SinGlass High (F18) in regenerating critical bone defects in rat calvaria. Both biomaterials promoted new bone formation around the particles, with the SinGlass High (F18) group exhibiting a higher rate of bone maturation. Histomorphological and birefringence analyses revealed better organization of the newly formed bone in the biomaterial-treated groups, and immunohistochemistry indicated the expression of osteogenic markers such as osteocalcin, immunostaining for bone morphogenetic protein 2 (BMP 2), and immunostaining for bone morphogenetic protein 4 (BMP 4). Microtomography computadorized (Micro-CT) revealed centripetal bone formation in both groups, with greater integration of the particles into the surrounding bone tissue. The superior performance of SinGlass High (F18) was attributed to its higher potassium and magnesium content, which enhance osteoconductivity. After 42 days, the SinGlass High (F18) group showed the highest percentage of new bone formation, in line with previous studies. Although our results are promising, the limited follow-up period and use of a single animal model highlight the need for further research to validate clinical applicability. SinGlass High (F18) appears to be a viable alternative to autografts in bone repair, with potential to improve tissue integration and accelerate recovery. |
| format | Article |
| id | doaj-art-286f13d4868244d58deb8a098dc9f64c |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-286f13d4868244d58deb8a098dc9f64c2025-01-24T13:25:14ZengMDPI AGBiomolecules2218-273X2025-01-0115111210.3390/biom15010112Biological Behavior of Bioactive Glasses SinGlass (45S5) and SinGlass High (F18) in the Repair of Critical Bone DefectsDayane Maria Braz Nogueira0Marcelie Priscila de Oliveira Rosso1Paulo Sérgio da Silva Santos2Manoel Damião Sousa-Neto3Alice Corrêa Silva-Sousa4Cleverson Teixeira Soares5Carlos Henrique Bertoni Reis6Jéssica de Oliveira Rossi7Cleuber Rodrigo de Souza Bueno8Daniela Vieira Buchaim9Rogério Leone Buchaim10Mariana Schutzer Ragghianti Zangrando11Department of Anatomy, Faculty of Higher Education of the Interior of São Paulo (FAIP), Marília 17512-130, BrazilDepartment of Physiotherapy, Physical Education, and Biomedicine, Estácio de Sá School of Ourinhos (FAESO), Ourinhos 19907-510, BrazilDepartment of Surgery, Stomatology, Pathology, and Radiology, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, BrazilDepartment of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, BrazilDepartment of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-904, BrazilLauro de Souza Lima Institute (ILSL), Anatomical Pathology Laboratory of Bauru (ANATOMED), Bauru 17034-971, BrazilBeneficent Hospital (HBU), University of Marilia (UNIMAR), Marilia 17525-160, BrazilOccupational Medicine, Marilia School of Medicine (FAMEMA), Marilia 17519-030, BrazilAnatomy Department, Faculty of Dentistry, University Center of Adamantina (FAI), Adamantina 17800-000, BrazilAnatomy Department, Medical School, University Center of Adamantina (FAI), Adamantina 17800-000, BrazilGraduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), Sao Paulo 05508-270, BrazilDepartment of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, BrazilThis study evaluated the osteogenic potential of the bioactive glasses SinGlass (45S5) and SinGlass High (F18) in regenerating critical bone defects in rat calvaria. Both biomaterials promoted new bone formation around the particles, with the SinGlass High (F18) group exhibiting a higher rate of bone maturation. Histomorphological and birefringence analyses revealed better organization of the newly formed bone in the biomaterial-treated groups, and immunohistochemistry indicated the expression of osteogenic markers such as osteocalcin, immunostaining for bone morphogenetic protein 2 (BMP 2), and immunostaining for bone morphogenetic protein 4 (BMP 4). Microtomography computadorized (Micro-CT) revealed centripetal bone formation in both groups, with greater integration of the particles into the surrounding bone tissue. The superior performance of SinGlass High (F18) was attributed to its higher potassium and magnesium content, which enhance osteoconductivity. After 42 days, the SinGlass High (F18) group showed the highest percentage of new bone formation, in line with previous studies. Although our results are promising, the limited follow-up period and use of a single animal model highlight the need for further research to validate clinical applicability. SinGlass High (F18) appears to be a viable alternative to autografts in bone repair, with potential to improve tissue integration and accelerate recovery.https://www.mdpi.com/2218-273X/15/1/112bone regenerationbioactive glassSinGlassSinGlass High (F18)osteogenesisbiomaterials |
| spellingShingle | Dayane Maria Braz Nogueira Marcelie Priscila de Oliveira Rosso Paulo Sérgio da Silva Santos Manoel Damião Sousa-Neto Alice Corrêa Silva-Sousa Cleverson Teixeira Soares Carlos Henrique Bertoni Reis Jéssica de Oliveira Rossi Cleuber Rodrigo de Souza Bueno Daniela Vieira Buchaim Rogério Leone Buchaim Mariana Schutzer Ragghianti Zangrando Biological Behavior of Bioactive Glasses SinGlass (45S5) and SinGlass High (F18) in the Repair of Critical Bone Defects Biomolecules bone regeneration bioactive glass SinGlass SinGlass High (F18) osteogenesis biomaterials |
| title | Biological Behavior of Bioactive Glasses SinGlass (45S5) and SinGlass High (F18) in the Repair of Critical Bone Defects |
| title_full | Biological Behavior of Bioactive Glasses SinGlass (45S5) and SinGlass High (F18) in the Repair of Critical Bone Defects |
| title_fullStr | Biological Behavior of Bioactive Glasses SinGlass (45S5) and SinGlass High (F18) in the Repair of Critical Bone Defects |
| title_full_unstemmed | Biological Behavior of Bioactive Glasses SinGlass (45S5) and SinGlass High (F18) in the Repair of Critical Bone Defects |
| title_short | Biological Behavior of Bioactive Glasses SinGlass (45S5) and SinGlass High (F18) in the Repair of Critical Bone Defects |
| title_sort | biological behavior of bioactive glasses singlass 45s5 and singlass high f18 in the repair of critical bone defects |
| topic | bone regeneration bioactive glass SinGlass SinGlass High (F18) osteogenesis biomaterials |
| url | https://www.mdpi.com/2218-273X/15/1/112 |
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