Predicting Tolerance to Cow’s Milk Allergy in Children Using IgE and IgG4 Peptide Binding Profiles
Cow’s milk allergy (CMA) is the most common food allergy in infants. This study aimed to identify peptide biomarkers predictive of tolerance in a Spanish population of children with CMA. We investigated specific IgE and IgG4 binding to sequential epitopes of the five major CM allergens (α-s1-, α-s2-...
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MDPI AG
2025-02-01
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| author | Carlos Fernández-Lozano Sergio Olmos-Piñero Laura Sánchez-Ruano Soledad Terrados Mª del Carmen Diéguez Montserrat Fernández-Rivas Cristina Vlaicu Inmaculada Cerecedo Alejandro Gonzalo-Fernandez Belén de la Hoz Javier Martínez-Botas |
| author_facet | Carlos Fernández-Lozano Sergio Olmos-Piñero Laura Sánchez-Ruano Soledad Terrados Mª del Carmen Diéguez Montserrat Fernández-Rivas Cristina Vlaicu Inmaculada Cerecedo Alejandro Gonzalo-Fernandez Belén de la Hoz Javier Martínez-Botas |
| author_sort | Carlos Fernández-Lozano |
| collection | DOAJ |
| description | Cow’s milk allergy (CMA) is the most common food allergy in infants. This study aimed to identify peptide biomarkers predictive of tolerance in a Spanish population of children with CMA. We investigated specific IgE and IgG4 binding to sequential epitopes of the five major CM allergens (α-s1-, α-s2-, β-, and κ-caseins as well as β-lactoglobulin) using a microarray-based immunoassay. Microarray analysis was performed in 118 patients at baseline and after 6, 18, 30, 42, and 54 months. Most patients tolerated CM at 6 months (40.7%) and 18 months (35.4%). We found significant differences in IgE and IgG4 binding intensity and diversity between allergic and tolerant patients. No differences were observed at baseline. Combining baseline IgE and IgG4 serology variables and peptide microarray analysis results, a predictive model was developed using the XGBoost algorithm to classify tolerance status at different time points. The generated models showed high predictive value at 6 and 30 months with AUCs of 0.883 and 0.833, respectively. Therefore, using IgE and IgG4 antibody-binding peptides at baseline, we generated two models predicting tolerance in children with cow’s milk allergy at 6 and 30 months. |
| format | Article |
| id | doaj-art-285e5e6999fa48b7b3c7f4a5b82bd439 |
| institution | DOAJ |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj-art-285e5e6999fa48b7b3c7f4a5b82bd4392025-08-20T02:53:19ZengMDPI AGCells2073-44092025-02-0114534410.3390/cells14050344Predicting Tolerance to Cow’s Milk Allergy in Children Using IgE and IgG4 Peptide Binding ProfilesCarlos Fernández-Lozano0Sergio Olmos-Piñero1Laura Sánchez-Ruano2Soledad Terrados3Mª del Carmen Diéguez4Montserrat Fernández-Rivas5Cristina Vlaicu6Inmaculada Cerecedo7Alejandro Gonzalo-Fernandez8Belén de la Hoz9Javier Martínez-Botas10Servicio de Bioquímica-Investigación, Hospital Universitario Ramón y Cajal, IRYCIS, 28034 Madrid, SpainServicio de Alergología, Hospital Universitario Ramón y Cajal, IRYCIS, 28034 Madrid, SpainServicio de Bioquímica-Investigación, Hospital Universitario Ramón y Cajal, IRYCIS, 28034 Madrid, SpainServicio de Alergología, Hospital Universitario Ramón y Cajal, IRYCIS, 28034 Madrid, SpainServicio de Alergología, Hospital Universitario Ramón y Cajal, IRYCIS, 28034 Madrid, SpainREI Network RD24/0007/0027, Instituto de Salud Carlos II, 28029 Madrid, SpainServicio de Alergología, Hospital Universitario Ramón y Cajal, IRYCIS, 28034 Madrid, SpainServicio de Alergología, Hospital Universitario del Sureste, 28500 Madrid, SpainREI Network RD24/0007/0027, Instituto de Salud Carlos II, 28029 Madrid, SpainServicio de Alergología, Hospital Universitario Ramón y Cajal, IRYCIS, 28034 Madrid, SpainServicio de Bioquímica-Investigación, Hospital Universitario Ramón y Cajal, IRYCIS, 28034 Madrid, SpainCow’s milk allergy (CMA) is the most common food allergy in infants. This study aimed to identify peptide biomarkers predictive of tolerance in a Spanish population of children with CMA. We investigated specific IgE and IgG4 binding to sequential epitopes of the five major CM allergens (α-s1-, α-s2-, β-, and κ-caseins as well as β-lactoglobulin) using a microarray-based immunoassay. Microarray analysis was performed in 118 patients at baseline and after 6, 18, 30, 42, and 54 months. Most patients tolerated CM at 6 months (40.7%) and 18 months (35.4%). We found significant differences in IgE and IgG4 binding intensity and diversity between allergic and tolerant patients. No differences were observed at baseline. Combining baseline IgE and IgG4 serology variables and peptide microarray analysis results, a predictive model was developed using the XGBoost algorithm to classify tolerance status at different time points. The generated models showed high predictive value at 6 and 30 months with AUCs of 0.883 and 0.833, respectively. Therefore, using IgE and IgG4 antibody-binding peptides at baseline, we generated two models predicting tolerance in children with cow’s milk allergy at 6 and 30 months.https://www.mdpi.com/2073-4409/14/5/344cow’s milk allergyIgEIgG4peptidebiomarkermicroarray |
| spellingShingle | Carlos Fernández-Lozano Sergio Olmos-Piñero Laura Sánchez-Ruano Soledad Terrados Mª del Carmen Diéguez Montserrat Fernández-Rivas Cristina Vlaicu Inmaculada Cerecedo Alejandro Gonzalo-Fernandez Belén de la Hoz Javier Martínez-Botas Predicting Tolerance to Cow’s Milk Allergy in Children Using IgE and IgG4 Peptide Binding Profiles Cells cow’s milk allergy IgE IgG4 peptide biomarker microarray |
| title | Predicting Tolerance to Cow’s Milk Allergy in Children Using IgE and IgG4 Peptide Binding Profiles |
| title_full | Predicting Tolerance to Cow’s Milk Allergy in Children Using IgE and IgG4 Peptide Binding Profiles |
| title_fullStr | Predicting Tolerance to Cow’s Milk Allergy in Children Using IgE and IgG4 Peptide Binding Profiles |
| title_full_unstemmed | Predicting Tolerance to Cow’s Milk Allergy in Children Using IgE and IgG4 Peptide Binding Profiles |
| title_short | Predicting Tolerance to Cow’s Milk Allergy in Children Using IgE and IgG4 Peptide Binding Profiles |
| title_sort | predicting tolerance to cow s milk allergy in children using ige and igg4 peptide binding profiles |
| topic | cow’s milk allergy IgE IgG4 peptide biomarker microarray |
| url | https://www.mdpi.com/2073-4409/14/5/344 |
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