Root zone diazotrophs of wheat in coastal saline soils from water-scarce regions of the Bohai Sea

Abstract The root-zone microbiome of wheat, encompassing root, rhizosphere and bulk soil communities, harbors diverse diazotrophs that critically enhance wheat nitrogen use efficiency, promote wheat growth, and improve abiotic stress tolerance. Despite their agricultural significance, the compositio...

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Main Authors: Huicheng Zhao, Meiyu Liu, Linqi Zhang, Xinhui Fu, Abraham Mulu Oljira
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05803-1
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author Huicheng Zhao
Meiyu Liu
Linqi Zhang
Xinhui Fu
Abraham Mulu Oljira
author_facet Huicheng Zhao
Meiyu Liu
Linqi Zhang
Xinhui Fu
Abraham Mulu Oljira
author_sort Huicheng Zhao
collection DOAJ
description Abstract The root-zone microbiome of wheat, encompassing root, rhizosphere and bulk soil communities, harbors diverse diazotrophs that critically enhance wheat nitrogen use efficiency, promote wheat growth, and improve abiotic stress tolerance. Despite their agricultural significance, the composition and temporal dynamics of these beneficial diazotroph communities in saline-alkali ecosystems remain poorly resolved, particularly under the stresses of aridity and soil salinity. Systematic profiling of wheat-associated diazotroph ecology is imperative for developing innovative solutions to enhance wheat resilience and advance sustainable agriculture. This study provides detailed microbiome data from wheat root-soil interface, consisting of root, rhizosphere and bulk soil samples collected from saline-alkali fields in the water-scarce Bohai Sea region of China. Through high-throughput sequencing of nifH gene, we generated an extensive dataset with an average of 60,265 high-quality reads per sample. The dataset provides a valuable resource for uncovering the recruitment strategies of wheat root-enriched diazotrophs to adapt to varying salinity stress and facilitating the development of novel microbiome based strategies for sustainable agricultural practices in saline-alkali ecosystems.
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spelling doaj-art-285bc2af7add44e5ad8f859cfb55cfbe2025-08-24T11:07:21ZengNature PortfolioScientific Data2052-44632025-08-011211610.1038/s41597-025-05803-1Root zone diazotrophs of wheat in coastal saline soils from water-scarce regions of the Bohai SeaHuicheng Zhao0Meiyu Liu1Linqi Zhang2Xinhui Fu3Abraham Mulu Oljira4Key Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesKey Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesKey Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesKey Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesKey Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesAbstract The root-zone microbiome of wheat, encompassing root, rhizosphere and bulk soil communities, harbors diverse diazotrophs that critically enhance wheat nitrogen use efficiency, promote wheat growth, and improve abiotic stress tolerance. Despite their agricultural significance, the composition and temporal dynamics of these beneficial diazotroph communities in saline-alkali ecosystems remain poorly resolved, particularly under the stresses of aridity and soil salinity. Systematic profiling of wheat-associated diazotroph ecology is imperative for developing innovative solutions to enhance wheat resilience and advance sustainable agriculture. This study provides detailed microbiome data from wheat root-soil interface, consisting of root, rhizosphere and bulk soil samples collected from saline-alkali fields in the water-scarce Bohai Sea region of China. Through high-throughput sequencing of nifH gene, we generated an extensive dataset with an average of 60,265 high-quality reads per sample. The dataset provides a valuable resource for uncovering the recruitment strategies of wheat root-enriched diazotrophs to adapt to varying salinity stress and facilitating the development of novel microbiome based strategies for sustainable agricultural practices in saline-alkali ecosystems.https://doi.org/10.1038/s41597-025-05803-1
spellingShingle Huicheng Zhao
Meiyu Liu
Linqi Zhang
Xinhui Fu
Abraham Mulu Oljira
Root zone diazotrophs of wheat in coastal saline soils from water-scarce regions of the Bohai Sea
Scientific Data
title Root zone diazotrophs of wheat in coastal saline soils from water-scarce regions of the Bohai Sea
title_full Root zone diazotrophs of wheat in coastal saline soils from water-scarce regions of the Bohai Sea
title_fullStr Root zone diazotrophs of wheat in coastal saline soils from water-scarce regions of the Bohai Sea
title_full_unstemmed Root zone diazotrophs of wheat in coastal saline soils from water-scarce regions of the Bohai Sea
title_short Root zone diazotrophs of wheat in coastal saline soils from water-scarce regions of the Bohai Sea
title_sort root zone diazotrophs of wheat in coastal saline soils from water scarce regions of the bohai sea
url https://doi.org/10.1038/s41597-025-05803-1
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