Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinoma
In this research, we investigated the role of PIK3R6, a regulatory subunit of PI3Kγ, known for its tumor-promoting properties, in clear cell renal cell carcinoma (CCRCC). Utilizing the UALCAN website, we found PIK3R6 upregulated in CCRCC, correlating with lower survival rates. We compared PIK3R6 exp...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Cell Adhesion & Migration |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19336918.2024.2353920 |
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| author | Jia Yang Xiaoni Zhong Xiaoling Gao Wenyi Xie Yaokai Chen Yuanjiang Liao Peilin Zhang |
| author_facet | Jia Yang Xiaoni Zhong Xiaoling Gao Wenyi Xie Yaokai Chen Yuanjiang Liao Peilin Zhang |
| author_sort | Jia Yang |
| collection | DOAJ |
| description | In this research, we investigated the role of PIK3R6, a regulatory subunit of PI3Kγ, known for its tumor-promoting properties, in clear cell renal cell carcinoma (CCRCC). Utilizing the UALCAN website, we found PIK3R6 upregulated in CCRCC, correlating with lower survival rates. We compared PIK3R6 expression in CCRCC tumor tissues and adjacent normal tissues using immunohistochemistry. Post RNA interference-induced knockdown of PIK3R6 in 786-O and ACHN cell lines, we performed CCK-8, colony formation, Edu staining, flow cytometry, wound healing, and transwell assays. Results showed that PIK3R6 silencing reduced cell proliferation, migration, and invasion, and induced G0/G1 phase arrest and apoptosis. Molecular analysis revealed decreased CDK4, Cyclin D1, N-cadherin, Vimentin, Bcl-2, p-PI3K and p-AKT, with increased cleaved caspase-3, Bax, and E-cadherin levels in CCRCC cells. Moreover, inhibiting PIK3R6 hindered tumor growth. These findings suggest a significant role for PIK3R6 in CCRCC cell proliferation and metastasis, presenting it as a potential therapeutic target. |
| format | Article |
| id | doaj-art-284ac985eee94b929a62c80fd646c616 |
| institution | OA Journals |
| issn | 1933-6918 1933-6926 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cell Adhesion & Migration |
| spelling | doaj-art-284ac985eee94b929a62c80fd646c6162025-08-20T02:33:07ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262024-12-0118111210.1080/19336918.2024.2353920Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinomaJia Yang0Xiaoni Zhong1Xiaoling Gao2Wenyi Xie3Yaokai Chen4Yuanjiang Liao5Peilin Zhang6College of Public Health, Chongqing Medical University, Chongqing, ChinaCollege of Public Health, Chongqing Medical University, Chongqing, ChinaDepartment of Nephrology, Chongqing Ninth People’s Hospital, Chongqing, ChinaDepartment of Nephrology, Chongqing Ninth People’s Hospital, Chongqing, ChinaScientific Research and Education Department, Chongqing Public Health Medical Center, Chongqing, ChinaDepartment of Nephrology, Chongqing Ninth People’s Hospital, Chongqing, ChinaCost Management Research Center, Chongqing Ninth People’s Hospital, Chongqing, ChinaIn this research, we investigated the role of PIK3R6, a regulatory subunit of PI3Kγ, known for its tumor-promoting properties, in clear cell renal cell carcinoma (CCRCC). Utilizing the UALCAN website, we found PIK3R6 upregulated in CCRCC, correlating with lower survival rates. We compared PIK3R6 expression in CCRCC tumor tissues and adjacent normal tissues using immunohistochemistry. Post RNA interference-induced knockdown of PIK3R6 in 786-O and ACHN cell lines, we performed CCK-8, colony formation, Edu staining, flow cytometry, wound healing, and transwell assays. Results showed that PIK3R6 silencing reduced cell proliferation, migration, and invasion, and induced G0/G1 phase arrest and apoptosis. Molecular analysis revealed decreased CDK4, Cyclin D1, N-cadherin, Vimentin, Bcl-2, p-PI3K and p-AKT, with increased cleaved caspase-3, Bax, and E-cadherin levels in CCRCC cells. Moreover, inhibiting PIK3R6 hindered tumor growth. These findings suggest a significant role for PIK3R6 in CCRCC cell proliferation and metastasis, presenting it as a potential therapeutic target.https://www.tandfonline.com/doi/10.1080/19336918.2024.2353920MigrationPIK3R6proliferationrenal cell carcinomatumor growth |
| spellingShingle | Jia Yang Xiaoni Zhong Xiaoling Gao Wenyi Xie Yaokai Chen Yuanjiang Liao Peilin Zhang Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinoma Cell Adhesion & Migration Migration PIK3R6 proliferation renal cell carcinoma tumor growth |
| title | Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinoma |
| title_full | Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinoma |
| title_fullStr | Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinoma |
| title_full_unstemmed | Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinoma |
| title_short | Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinoma |
| title_sort | knockdown of pik3r6 impedes the onset and advancement of clear cell renal cell carcinoma |
| topic | Migration PIK3R6 proliferation renal cell carcinoma tumor growth |
| url | https://www.tandfonline.com/doi/10.1080/19336918.2024.2353920 |
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