Dynamic Tracking Human Mesenchymal Stem Cells Tropism following Smoke Inhalation Injury in NOD/SCID Mice
Multiple preclinical evidences have supported the potential value of mesenchymal stem cells (MSCs) for treatment of acute lung injury (ALI). However, few studies focus on the dynamic tropism of MSCs in animals with acute lung injury. In this study, we track systemically transplanted human bone marro...
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| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/1691856 |
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| author | MeiJuan Song Qi Lv XiuWei Zhang Juan Cao ShuLi Sun PeiXin Xiao ShiKe Hou Hui Ding ZiQuan Liu WenLong Dong JinQiang Wang Xue Wang ZhiGuang Sun Man Tian HaoJun Fan |
| author_facet | MeiJuan Song Qi Lv XiuWei Zhang Juan Cao ShuLi Sun PeiXin Xiao ShiKe Hou Hui Ding ZiQuan Liu WenLong Dong JinQiang Wang Xue Wang ZhiGuang Sun Man Tian HaoJun Fan |
| author_sort | MeiJuan Song |
| collection | DOAJ |
| description | Multiple preclinical evidences have supported the potential value of mesenchymal stem cells (MSCs) for treatment of acute lung injury (ALI). However, few studies focus on the dynamic tropism of MSCs in animals with acute lung injury. In this study, we track systemically transplanted human bone marrow-derived mesenchymal stem cells (hBMSCs) in NOD/SCID mice with smoke inhalation injury (SII) through bioluminescence imaging (BLI). The results showed that hBMSCs systemically delivered into healthy NOD/SCID mouse initially reside in the lungs and then partially translocate to the abdomen after 24 h. Compared with the uninjured control group treated with hBMSCs, higher numbers of hBMSCs were found in the lungs of the SII NOD/SCID mice. In both the uninjured and SII mice, the BLI signals in the lungs steadily decreased over time and disappeared by 5 days after treatment. hBMSCs significantly attenuated lung injury, elevated the levels of KGF, decreased the levels of TNF-α in BALF, and inhibited inflammatory cell infiltration in the mice with SII. In conclusion, our findings demonstrated that more systemically infused hBMSCs localized to the lungs in mice with SII. hBMSC xenografts repaired smoke inhalation-induced lung injury in mice. This repair was maybe due to the effect of anti-inflammatory and secreting KGF of hMSCs but not associated with the differentiation of the hBMSCs into alveolar epithelial cells. |
| format | Article |
| id | doaj-art-28474e5253a64854a53603400236ec31 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-28474e5253a64854a53603400236ec312025-02-03T01:23:01ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/16918561691856Dynamic Tracking Human Mesenchymal Stem Cells Tropism following Smoke Inhalation Injury in NOD/SCID MiceMeiJuan Song0Qi Lv1XiuWei Zhang2Juan Cao3ShuLi Sun4PeiXin Xiao5ShiKe Hou6Hui Ding7ZiQuan Liu8WenLong Dong9JinQiang Wang10Xue Wang11ZhiGuang Sun12Man Tian13HaoJun Fan14Respiratory Department, Affiliated Jiangning Hospital, Nanjing Medical University, Jiangsu, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaRespiratory Department, Affiliated Jiangning Hospital, Nanjing Medical University, Jiangsu, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaRespiratory Department, Affiliated Nanjing Children’s Hospital, Nanjing Medical University, Jiangsu, ChinaInstitute of Disaster Medicine and Public Health, Affiliated Hospital of Logistic University of Chinese People’s Armed Police Force, Tianjin 300162, ChinaMultiple preclinical evidences have supported the potential value of mesenchymal stem cells (MSCs) for treatment of acute lung injury (ALI). However, few studies focus on the dynamic tropism of MSCs in animals with acute lung injury. In this study, we track systemically transplanted human bone marrow-derived mesenchymal stem cells (hBMSCs) in NOD/SCID mice with smoke inhalation injury (SII) through bioluminescence imaging (BLI). The results showed that hBMSCs systemically delivered into healthy NOD/SCID mouse initially reside in the lungs and then partially translocate to the abdomen after 24 h. Compared with the uninjured control group treated with hBMSCs, higher numbers of hBMSCs were found in the lungs of the SII NOD/SCID mice. In both the uninjured and SII mice, the BLI signals in the lungs steadily decreased over time and disappeared by 5 days after treatment. hBMSCs significantly attenuated lung injury, elevated the levels of KGF, decreased the levels of TNF-α in BALF, and inhibited inflammatory cell infiltration in the mice with SII. In conclusion, our findings demonstrated that more systemically infused hBMSCs localized to the lungs in mice with SII. hBMSC xenografts repaired smoke inhalation-induced lung injury in mice. This repair was maybe due to the effect of anti-inflammatory and secreting KGF of hMSCs but not associated with the differentiation of the hBMSCs into alveolar epithelial cells.http://dx.doi.org/10.1155/2016/1691856 |
| spellingShingle | MeiJuan Song Qi Lv XiuWei Zhang Juan Cao ShuLi Sun PeiXin Xiao ShiKe Hou Hui Ding ZiQuan Liu WenLong Dong JinQiang Wang Xue Wang ZhiGuang Sun Man Tian HaoJun Fan Dynamic Tracking Human Mesenchymal Stem Cells Tropism following Smoke Inhalation Injury in NOD/SCID Mice Stem Cells International |
| title | Dynamic Tracking Human Mesenchymal Stem Cells Tropism following Smoke Inhalation Injury in NOD/SCID Mice |
| title_full | Dynamic Tracking Human Mesenchymal Stem Cells Tropism following Smoke Inhalation Injury in NOD/SCID Mice |
| title_fullStr | Dynamic Tracking Human Mesenchymal Stem Cells Tropism following Smoke Inhalation Injury in NOD/SCID Mice |
| title_full_unstemmed | Dynamic Tracking Human Mesenchymal Stem Cells Tropism following Smoke Inhalation Injury in NOD/SCID Mice |
| title_short | Dynamic Tracking Human Mesenchymal Stem Cells Tropism following Smoke Inhalation Injury in NOD/SCID Mice |
| title_sort | dynamic tracking human mesenchymal stem cells tropism following smoke inhalation injury in nod scid mice |
| url | http://dx.doi.org/10.1155/2016/1691856 |
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