Farming Practices, Biosecurity Gaps, and Genetic Insights into African Swine Fever Virus in the Iringa and Ruvuma Regions of Tanzania

African Swine Fever Virus (ASFV) genotype II dominates outbreaks in Tanzania’s Southern Highlands, continuing to persist as the dominant strain over a decade after its first incursion in 2010. A total of 205 samples from 120 holdings were collected, with 21 confirmed ASFV-positive animals from 14 ho...

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Main Authors: Agathe Auer, Anderson Samwel Yohana, Tirumala B. K. Settypalli, Raphael Sallu, Jelly Chang’a, Stella Bitanyi, Stella Gaichugi Kiambi, Irene K. Meki, William G. Dundon, Artem Metlin, Andriy Rozstalnyy, Geofrey Hallan Mbata, James Anset Okachu, Henry Magwisha, Sauda Ally Hamis, Jeremia Theodos Choga, Stela Lucas Chalo, Joshua Kimutai, Gerald Misinzo, Solomon Wilson Nong’ona, Joseph Edmund Lyimo, Charles E. Lamien
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/7/1007
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author Agathe Auer
Anderson Samwel Yohana
Tirumala B. K. Settypalli
Raphael Sallu
Jelly Chang’a
Stella Bitanyi
Stella Gaichugi Kiambi
Irene K. Meki
William G. Dundon
Artem Metlin
Andriy Rozstalnyy
Geofrey Hallan Mbata
James Anset Okachu
Henry Magwisha
Sauda Ally Hamis
Jeremia Theodos Choga
Stela Lucas Chalo
Joshua Kimutai
Gerald Misinzo
Solomon Wilson Nong’ona
Joseph Edmund Lyimo
Charles E. Lamien
author_facet Agathe Auer
Anderson Samwel Yohana
Tirumala B. K. Settypalli
Raphael Sallu
Jelly Chang’a
Stella Bitanyi
Stella Gaichugi Kiambi
Irene K. Meki
William G. Dundon
Artem Metlin
Andriy Rozstalnyy
Geofrey Hallan Mbata
James Anset Okachu
Henry Magwisha
Sauda Ally Hamis
Jeremia Theodos Choga
Stela Lucas Chalo
Joshua Kimutai
Gerald Misinzo
Solomon Wilson Nong’ona
Joseph Edmund Lyimo
Charles E. Lamien
author_sort Agathe Auer
collection DOAJ
description African Swine Fever Virus (ASFV) genotype II dominates outbreaks in Tanzania’s Southern Highlands, continuing to persist as the dominant strain over a decade after its first incursion in 2010. A total of 205 samples from 120 holdings were collected, with 21 confirmed ASFV-positive animals from 14 holdings. Molecular analysis revealed genetic uniformity among isolates, all clustering within ASFV genotype II. Poor biosecurity measures, such as feeding of untreated swill (80% of holdings) and lack of restrictions on visitors (90% of holdings), were identified as risk factors. Additionally, co-infection with porcine circovirus-2 (PCV-2) further complicates disease management. This study underscores the urgent need for enhanced biosecurity and farmer education to mitigate ASFV outbreaks in endemic regions.
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spelling doaj-art-283fba2875e941709c5724ecd41fb6da2025-08-20T02:09:18ZengMDPI AGAnimals2076-26152025-03-01157100710.3390/ani15071007Farming Practices, Biosecurity Gaps, and Genetic Insights into African Swine Fever Virus in the Iringa and Ruvuma Regions of TanzaniaAgathe Auer0Anderson Samwel Yohana1Tirumala B. K. Settypalli2Raphael Sallu3Jelly Chang’a4Stella Bitanyi5Stella Gaichugi Kiambi6Irene K. Meki7William G. Dundon8Artem Metlin9Andriy Rozstalnyy10Geofrey Hallan Mbata11James Anset Okachu12Henry Magwisha13Sauda Ally Hamis14Jeremia Theodos Choga15Stela Lucas Chalo16Joshua Kimutai17Gerald Misinzo18Solomon Wilson Nong’ona19Joseph Edmund Lyimo20Charles E. Lamien21Animal Production and Health Laboratory (APHL) at the Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency (IAEA), Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaTanzania Veterinary Laboratory Agency Iringa (TVLA), Iringa P.O. Box 290, TanzaniaAnimal Production and Health Laboratory (APHL) at the Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency (IAEA), Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaFood and Agriculture Organization of the United Nations (FAO), Emergency Centre for Transboundary Animal Diseases, Dar Es Salaam 14111, TanzaniaTanzania Veterinary Laboratory Agency Iringa (TVLA), Iringa P.O. Box 290, TanzaniaTanzania Veterinary Laboratory Agency Iringa (TVLA), Iringa P.O. Box 290, TanzaniaFood and Agriculture Organization of the United Nations (FAO), Emergency Centre for Transboundary Animal Diseases, Dar Es Salaam 14111, TanzaniaAnimal Production and Health Laboratory (APHL) at the Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency (IAEA), Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaAnimal Production and Health Laboratory (APHL) at the Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency (IAEA), Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaFood and Agriculture Organization of the United Nations (FAO), 00153 Rome, ItalyFood and Agriculture Organization of the United Nations (FAO), 00153 Rome, ItalyTanzania Veterinary Laboratory Agency Iringa (TVLA), Iringa P.O. Box 290, TanzaniaTanzania Veterinary Laboratory Agency Iringa (TVLA), Iringa P.O. Box 290, TanzaniaFood and Agriculture Organization of the United Nations (FAO), Emergency Centre for Transboundary Animal Diseases, Dar Es Salaam 14111, TanzaniaTanzania Veterinary Laboratory Agency Iringa (TVLA), Iringa P.O. Box 290, TanzaniaZonal Veterinary Investigation Centre (ZVC), Southern Highland Zone, Iringa P.O. Box 290, TanzaniaZonal Veterinary Investigation Centre (ZVC), Southern Highland Zone, Iringa P.O. Box 290, TanzaniaFood and Agriculture Organization of the United Nations (FAO), Emergency Centre for Transboundary Animal Diseases, Nairobi 00100, KenyaDepartment of Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, P.O. Box 3019, Morogoro 67152, TanzaniaZonal Veterinary Investigation Centre (ZVC), Southern Highland Zone, Iringa P.O. Box 290, TanzaniaZonal Veterinary Investigation Centre (ZVC), Southern Highland Zone, Iringa P.O. Box 290, TanzaniaAnimal Production and Health Laboratory (APHL) at the Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency (IAEA), Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaAfrican Swine Fever Virus (ASFV) genotype II dominates outbreaks in Tanzania’s Southern Highlands, continuing to persist as the dominant strain over a decade after its first incursion in 2010. A total of 205 samples from 120 holdings were collected, with 21 confirmed ASFV-positive animals from 14 holdings. Molecular analysis revealed genetic uniformity among isolates, all clustering within ASFV genotype II. Poor biosecurity measures, such as feeding of untreated swill (80% of holdings) and lack of restrictions on visitors (90% of holdings), were identified as risk factors. Additionally, co-infection with porcine circovirus-2 (PCV-2) further complicates disease management. This study underscores the urgent need for enhanced biosecurity and farmer education to mitigate ASFV outbreaks in endemic regions.https://www.mdpi.com/2076-2615/15/7/1007ASFVbiosecuritymolecular characterizationTanzaniapig farming
spellingShingle Agathe Auer
Anderson Samwel Yohana
Tirumala B. K. Settypalli
Raphael Sallu
Jelly Chang’a
Stella Bitanyi
Stella Gaichugi Kiambi
Irene K. Meki
William G. Dundon
Artem Metlin
Andriy Rozstalnyy
Geofrey Hallan Mbata
James Anset Okachu
Henry Magwisha
Sauda Ally Hamis
Jeremia Theodos Choga
Stela Lucas Chalo
Joshua Kimutai
Gerald Misinzo
Solomon Wilson Nong’ona
Joseph Edmund Lyimo
Charles E. Lamien
Farming Practices, Biosecurity Gaps, and Genetic Insights into African Swine Fever Virus in the Iringa and Ruvuma Regions of Tanzania
Animals
ASFV
biosecurity
molecular characterization
Tanzania
pig farming
title Farming Practices, Biosecurity Gaps, and Genetic Insights into African Swine Fever Virus in the Iringa and Ruvuma Regions of Tanzania
title_full Farming Practices, Biosecurity Gaps, and Genetic Insights into African Swine Fever Virus in the Iringa and Ruvuma Regions of Tanzania
title_fullStr Farming Practices, Biosecurity Gaps, and Genetic Insights into African Swine Fever Virus in the Iringa and Ruvuma Regions of Tanzania
title_full_unstemmed Farming Practices, Biosecurity Gaps, and Genetic Insights into African Swine Fever Virus in the Iringa and Ruvuma Regions of Tanzania
title_short Farming Practices, Biosecurity Gaps, and Genetic Insights into African Swine Fever Virus in the Iringa and Ruvuma Regions of Tanzania
title_sort farming practices biosecurity gaps and genetic insights into african swine fever virus in the iringa and ruvuma regions of tanzania
topic ASFV
biosecurity
molecular characterization
Tanzania
pig farming
url https://www.mdpi.com/2076-2615/15/7/1007
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