Combined effects of acidification and warming on soil denitrification and microbial community
In light of the challenges posed by contemporary global warming and soil acidification, the respective effects of pH and temperature on soil microbiome and functions have been explored. However, the combined influence of acidification and warming on soil denitrification and active microbial communit...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1572497/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849738314921279488 |
|---|---|
| author | Peiyuan Xu Peiyuan Xu Mengke Gao Mengke Gao Yuchen Li Yuchen Li Jun Ye Jianqiang Su Jianqiang Su Hu Li Hu Li |
| author_facet | Peiyuan Xu Peiyuan Xu Mengke Gao Mengke Gao Yuchen Li Yuchen Li Jun Ye Jianqiang Su Jianqiang Su Hu Li Hu Li |
| author_sort | Peiyuan Xu |
| collection | DOAJ |
| description | In light of the challenges posed by contemporary global warming and soil acidification, the respective effects of pH and temperature on soil microbiome and functions have been explored. However, the combined influence of acidification and warming on soil denitrification and active microbial communities are still unclear. Here, we conducted a microcosm experiment to investigate the influences of increasing temperature and acidification on active microbes such as bacteria and eukaryotic microbes. Denitrification rate in soil were detected using a C2H2 inhibition method. The results showed that the Shannon index of bacterial communities exhibited significant enhancement in response to warming and acidification, whereas their community patterns were predominantly shaped by pH. For the micro-eukaryotic community, temperature emerged as the main driver of variations in the α-diversity, with the MT group exhibiting significantly lower Shannon indices compared to LT and HT groups. Both pH and temperature exerted a combined effect on their community patterns. Additionally, pH was detected as a crucial factor influencing denitrification rates, with a significant negative correlation between pH and denitrification rate within the pH range of 4.32–7.46 across all temperatures in this study. Our findings highlighted the significant impacts of acidification on soil denitrification rates and active microbes under global warming, which provided an important scientific basis for agricultural production management and environmental protection in the context of global climate warming. |
| format | Article |
| id | doaj-art-283f7bf16f774161b762c5b9b9661ef8 |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-283f7bf16f774161b762c5b9b9661ef82025-08-20T03:06:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-04-011610.3389/fmicb.2025.15724971572497Combined effects of acidification and warming on soil denitrification and microbial communityPeiyuan Xu0Peiyuan Xu1Mengke Gao2Mengke Gao3Yuchen Li4Yuchen Li5Jun Ye6Jianqiang Su7Jianqiang Su8Hu Li9Hu Li10State Key Laboratory for Ecological Security of Regions and Cities, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaSchool of Life Sciences, Hebei University, Baoding, ChinaState Key Laboratory for Ecological Security of Regions and Cities, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaCollege of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory for Ecological Security of Regions and Cities, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaSchool of Life Sciences, Hebei University, Baoding, ChinaSchool of Life Sciences, Hebei University, Baoding, ChinaState Key Laboratory for Ecological Security of Regions and Cities, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory for Ecological Security of Regions and Cities, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaIn light of the challenges posed by contemporary global warming and soil acidification, the respective effects of pH and temperature on soil microbiome and functions have been explored. However, the combined influence of acidification and warming on soil denitrification and active microbial communities are still unclear. Here, we conducted a microcosm experiment to investigate the influences of increasing temperature and acidification on active microbes such as bacteria and eukaryotic microbes. Denitrification rate in soil were detected using a C2H2 inhibition method. The results showed that the Shannon index of bacterial communities exhibited significant enhancement in response to warming and acidification, whereas their community patterns were predominantly shaped by pH. For the micro-eukaryotic community, temperature emerged as the main driver of variations in the α-diversity, with the MT group exhibiting significantly lower Shannon indices compared to LT and HT groups. Both pH and temperature exerted a combined effect on their community patterns. Additionally, pH was detected as a crucial factor influencing denitrification rates, with a significant negative correlation between pH and denitrification rate within the pH range of 4.32–7.46 across all temperatures in this study. Our findings highlighted the significant impacts of acidification on soil denitrification rates and active microbes under global warming, which provided an important scientific basis for agricultural production management and environmental protection in the context of global climate warming.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1572497/fullacidificationwarmingRNA levelmicrobial communitydenitrification rate |
| spellingShingle | Peiyuan Xu Peiyuan Xu Mengke Gao Mengke Gao Yuchen Li Yuchen Li Jun Ye Jianqiang Su Jianqiang Su Hu Li Hu Li Combined effects of acidification and warming on soil denitrification and microbial community Frontiers in Microbiology acidification warming RNA level microbial community denitrification rate |
| title | Combined effects of acidification and warming on soil denitrification and microbial community |
| title_full | Combined effects of acidification and warming on soil denitrification and microbial community |
| title_fullStr | Combined effects of acidification and warming on soil denitrification and microbial community |
| title_full_unstemmed | Combined effects of acidification and warming on soil denitrification and microbial community |
| title_short | Combined effects of acidification and warming on soil denitrification and microbial community |
| title_sort | combined effects of acidification and warming on soil denitrification and microbial community |
| topic | acidification warming RNA level microbial community denitrification rate |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1572497/full |
| work_keys_str_mv | AT peiyuanxu combinedeffectsofacidificationandwarmingonsoildenitrificationandmicrobialcommunity AT peiyuanxu combinedeffectsofacidificationandwarmingonsoildenitrificationandmicrobialcommunity AT mengkegao combinedeffectsofacidificationandwarmingonsoildenitrificationandmicrobialcommunity AT mengkegao combinedeffectsofacidificationandwarmingonsoildenitrificationandmicrobialcommunity AT yuchenli combinedeffectsofacidificationandwarmingonsoildenitrificationandmicrobialcommunity AT yuchenli combinedeffectsofacidificationandwarmingonsoildenitrificationandmicrobialcommunity AT junye combinedeffectsofacidificationandwarmingonsoildenitrificationandmicrobialcommunity AT jianqiangsu combinedeffectsofacidificationandwarmingonsoildenitrificationandmicrobialcommunity AT jianqiangsu combinedeffectsofacidificationandwarmingonsoildenitrificationandmicrobialcommunity AT huli combinedeffectsofacidificationandwarmingonsoildenitrificationandmicrobialcommunity AT huli combinedeffectsofacidificationandwarmingonsoildenitrificationandmicrobialcommunity |