Identification of Clonorchis sinensis in bronchoalveolar lavage fluid and peripheral blood using next-generation sequencing in a patient with septic shock: a case report and literature review

We present a case report of a 59-year-old male diagnosed with pancreatic cancer with multiple abdominal metastases, in whom metagenomic next-generation sequencing unexpectedly identified Clonorchis sinensis genomic sequences in both bronchoalveolar lavage fluid and peripheral blood specimens. Subseq...

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Main Authors: Weipeng Liu, Mengting Su, Dongjing Zhang, Siqi Liu, Yin Zhang, Zhengfei Yang
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Emerging Microbes and Infections
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/22221751.2025.2511133
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author Weipeng Liu
Mengting Su
Dongjing Zhang
Siqi Liu
Yin Zhang
Zhengfei Yang
author_facet Weipeng Liu
Mengting Su
Dongjing Zhang
Siqi Liu
Yin Zhang
Zhengfei Yang
author_sort Weipeng Liu
collection DOAJ
description We present a case report of a 59-year-old male diagnosed with pancreatic cancer with multiple abdominal metastases, in whom metagenomic next-generation sequencing unexpectedly identified Clonorchis sinensis genomic sequences in both bronchoalveolar lavage fluid and peripheral blood specimens. Subsequent examination further confirmed the presence of viable C. sinensis eggs in the stool samples. The findings underscore the prognostic significance of enhanced diagnostic protocols for parasitic infections in oncological cases. This investigation demonstrates the clinical utility of molecular diagnostic approaches and alternative biological specimens for detecting C. sinensis infection.
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publisher Taylor & Francis Group
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series Emerging Microbes and Infections
spelling doaj-art-283dd58dbdb84fad8a31abea8e1753fb2025-08-20T03:07:35ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512025-12-0114110.1080/22221751.2025.2511133Identification of Clonorchis sinensis in bronchoalveolar lavage fluid and peripheral blood using next-generation sequencing in a patient with septic shock: a case report and literature reviewWeipeng Liu0Mengting Su1Dongjing Zhang2Siqi Liu3Yin Zhang4Zhengfei Yang5Department of Emergency Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaCellular & Molecular Diagnostics Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaChinese Atomic Energy Agency Centre of Excellence on Nuclear Technology Applications for Insect Control, Key Laboratory of Tropical Disease Control of the Ministry of Education, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaDepartment of Emergency Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaCellular & Molecular Diagnostics Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaDepartment of Emergency Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaWe present a case report of a 59-year-old male diagnosed with pancreatic cancer with multiple abdominal metastases, in whom metagenomic next-generation sequencing unexpectedly identified Clonorchis sinensis genomic sequences in both bronchoalveolar lavage fluid and peripheral blood specimens. Subsequent examination further confirmed the presence of viable C. sinensis eggs in the stool samples. The findings underscore the prognostic significance of enhanced diagnostic protocols for parasitic infections in oncological cases. This investigation demonstrates the clinical utility of molecular diagnostic approaches and alternative biological specimens for detecting C. sinensis infection.https://www.tandfonline.com/doi/10.1080/22221751.2025.2511133Clonorchis sinensisnext-generation sequencingpancreatic cancerar lavage fluidperiphera blood
spellingShingle Weipeng Liu
Mengting Su
Dongjing Zhang
Siqi Liu
Yin Zhang
Zhengfei Yang
Identification of Clonorchis sinensis in bronchoalveolar lavage fluid and peripheral blood using next-generation sequencing in a patient with septic shock: a case report and literature review
Emerging Microbes and Infections
Clonorchis sinensis
next-generation sequencing
pancreatic cancer
ar lavage fluid
periphera blood
title Identification of Clonorchis sinensis in bronchoalveolar lavage fluid and peripheral blood using next-generation sequencing in a patient with septic shock: a case report and literature review
title_full Identification of Clonorchis sinensis in bronchoalveolar lavage fluid and peripheral blood using next-generation sequencing in a patient with septic shock: a case report and literature review
title_fullStr Identification of Clonorchis sinensis in bronchoalveolar lavage fluid and peripheral blood using next-generation sequencing in a patient with septic shock: a case report and literature review
title_full_unstemmed Identification of Clonorchis sinensis in bronchoalveolar lavage fluid and peripheral blood using next-generation sequencing in a patient with septic shock: a case report and literature review
title_short Identification of Clonorchis sinensis in bronchoalveolar lavage fluid and peripheral blood using next-generation sequencing in a patient with septic shock: a case report and literature review
title_sort identification of clonorchis sinensis in bronchoalveolar lavage fluid and peripheral blood using next generation sequencing in a patient with septic shock a case report and literature review
topic Clonorchis sinensis
next-generation sequencing
pancreatic cancer
ar lavage fluid
periphera blood
url https://www.tandfonline.com/doi/10.1080/22221751.2025.2511133
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