Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India
This study is to probe the pattern of antibiotic resistance against aminoglycosides and its mechanism in E. coli obtained from patients from Chennai, India. Isolation and identification of pathogens were done on MacConkey agar. Antimicrobial sensitivity testing was done by disc diffusion test. The i...
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Wiley
2016-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2016/1875865 |
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| author | Abdul Rouf Mir Yasir Bashir Firdous Ahmad Dar M. Sekhar |
| author_facet | Abdul Rouf Mir Yasir Bashir Firdous Ahmad Dar M. Sekhar |
| author_sort | Abdul Rouf Mir |
| collection | DOAJ |
| description | This study is to probe the pattern of antibiotic resistance against aminoglycosides and its mechanism in E. coli obtained from patients from Chennai, India. Isolation and identification of pathogens were done on MacConkey agar. Antimicrobial sensitivity testing was done by disc diffusion test. The identification of genes encoding aminoglycoside modifying enzymes was done by Polymerase Chain Reaction (PCR). Out of 98 isolates, 71 (72.45%) isolates were identified as E. coli and the remaining 27 (27.55%) as other bacteria. Disc diffusion method results showed a resistance level of 72.15% for streptomycin, 73.4% for gentamicin, 63.26% for neomycin, 57.14% for tobramycin, 47.9% for netilmicin, and 8.16% for amikacin in E. coli. PCR screening showed the presence of four genes, namely, rrs, aacC2, aacA-aphD, and aphA3, in their plasmid DNA. The results point towards the novel mechanism of drug resistance in E. coli from UTI patients in India as they confirm the presence of genes encoding enzymes that cause resistance to aminoglycoside drugs. This could be an alarm for drug prescription to UTI patients. |
| format | Article |
| id | doaj-art-283af085bdce45389ffb7f258743b3d5 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-283af085bdce45389ffb7f258743b3d52025-08-20T03:54:38ZengWileyThe Scientific World Journal2356-61401537-744X2016-01-01201610.1155/2016/18758651875865Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in IndiaAbdul Rouf Mir0Yasir Bashir1Firdous Ahmad Dar2M. Sekhar3Department of Biotechnology, Government Degree College Baramulla, Kashmir 193101, IndiaDepartment of Molecular Biology and Biotechnology, Tezpur University, Assam, IndiaDepartment of Biotechnology, Government Degree College Baramulla, Kashmir 193101, IndiaDepartment of Biotechnology, The New College, Chennai, IndiaThis study is to probe the pattern of antibiotic resistance against aminoglycosides and its mechanism in E. coli obtained from patients from Chennai, India. Isolation and identification of pathogens were done on MacConkey agar. Antimicrobial sensitivity testing was done by disc diffusion test. The identification of genes encoding aminoglycoside modifying enzymes was done by Polymerase Chain Reaction (PCR). Out of 98 isolates, 71 (72.45%) isolates were identified as E. coli and the remaining 27 (27.55%) as other bacteria. Disc diffusion method results showed a resistance level of 72.15% for streptomycin, 73.4% for gentamicin, 63.26% for neomycin, 57.14% for tobramycin, 47.9% for netilmicin, and 8.16% for amikacin in E. coli. PCR screening showed the presence of four genes, namely, rrs, aacC2, aacA-aphD, and aphA3, in their plasmid DNA. The results point towards the novel mechanism of drug resistance in E. coli from UTI patients in India as they confirm the presence of genes encoding enzymes that cause resistance to aminoglycoside drugs. This could be an alarm for drug prescription to UTI patients.http://dx.doi.org/10.1155/2016/1875865 |
| spellingShingle | Abdul Rouf Mir Yasir Bashir Firdous Ahmad Dar M. Sekhar Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India The Scientific World Journal |
| title | Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India |
| title_full | Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India |
| title_fullStr | Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India |
| title_full_unstemmed | Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India |
| title_short | Identification of Genes Coding Aminoglycoside Modifying Enzymes in E. coli of UTI Patients in India |
| title_sort | identification of genes coding aminoglycoside modifying enzymes in e coli of uti patients in india |
| url | http://dx.doi.org/10.1155/2016/1875865 |
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