Phosphatidylserine synthase regulates cellular homeostasis through distinct metabolic mechanisms.
Phosphatidylserine (PS), synthesized in the endoplasmic reticulum (ER) by phosphatidylserine synthase (PSS), is transported to the plasma membrane (PM) and mitochondria through distinct routes. The in vivo functions of PS at different subcellular locations and the coordination between different PS t...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-12-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1008548&type=printable |
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| author | Xiao Yang Jingjing Liang Long Ding Xia Li Sin-Man Lam Guanghou Shui Mei Ding Xun Huang |
| author_facet | Xiao Yang Jingjing Liang Long Ding Xia Li Sin-Man Lam Guanghou Shui Mei Ding Xun Huang |
| author_sort | Xiao Yang |
| collection | DOAJ |
| description | Phosphatidylserine (PS), synthesized in the endoplasmic reticulum (ER) by phosphatidylserine synthase (PSS), is transported to the plasma membrane (PM) and mitochondria through distinct routes. The in vivo functions of PS at different subcellular locations and the coordination between different PS transport routes are not fully understood. Here, we report that Drosophila PSS regulates cell growth, lipid storage and mitochondrial function. In pss RNAi, reduced PS depletes plasma membrane Akt, contributing to cell growth defects; the metabolic shift from phospholipid synthesis to neutral lipid synthesis results in ectopic lipid accumulation; and the reduction of mitochondrial PS impairs mitochondrial protein import and mitochondrial integrity. Importantly, reducing PS transport from the ER to PM by loss of PI4KIIIα partially rescues the mitochondrial defects of pss RNAi. Together, our results uncover a balance between different PS transport routes and reveal that PSS regulates cellular homeostasis through distinct metabolic mechanisms. |
| format | Article |
| id | doaj-art-283ae42a327b48048daf2a2175f0c770 |
| institution | OA Journals |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-283ae42a327b48048daf2a2175f0c7702025-08-20T02:11:01ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042019-12-011512e100854810.1371/journal.pgen.1008548Phosphatidylserine synthase regulates cellular homeostasis through distinct metabolic mechanisms.Xiao YangJingjing LiangLong DingXia LiSin-Man LamGuanghou ShuiMei DingXun HuangPhosphatidylserine (PS), synthesized in the endoplasmic reticulum (ER) by phosphatidylserine synthase (PSS), is transported to the plasma membrane (PM) and mitochondria through distinct routes. The in vivo functions of PS at different subcellular locations and the coordination between different PS transport routes are not fully understood. Here, we report that Drosophila PSS regulates cell growth, lipid storage and mitochondrial function. In pss RNAi, reduced PS depletes plasma membrane Akt, contributing to cell growth defects; the metabolic shift from phospholipid synthesis to neutral lipid synthesis results in ectopic lipid accumulation; and the reduction of mitochondrial PS impairs mitochondrial protein import and mitochondrial integrity. Importantly, reducing PS transport from the ER to PM by loss of PI4KIIIα partially rescues the mitochondrial defects of pss RNAi. Together, our results uncover a balance between different PS transport routes and reveal that PSS regulates cellular homeostasis through distinct metabolic mechanisms.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1008548&type=printable |
| spellingShingle | Xiao Yang Jingjing Liang Long Ding Xia Li Sin-Man Lam Guanghou Shui Mei Ding Xun Huang Phosphatidylserine synthase regulates cellular homeostasis through distinct metabolic mechanisms. PLoS Genetics |
| title | Phosphatidylserine synthase regulates cellular homeostasis through distinct metabolic mechanisms. |
| title_full | Phosphatidylserine synthase regulates cellular homeostasis through distinct metabolic mechanisms. |
| title_fullStr | Phosphatidylserine synthase regulates cellular homeostasis through distinct metabolic mechanisms. |
| title_full_unstemmed | Phosphatidylserine synthase regulates cellular homeostasis through distinct metabolic mechanisms. |
| title_short | Phosphatidylserine synthase regulates cellular homeostasis through distinct metabolic mechanisms. |
| title_sort | phosphatidylserine synthase regulates cellular homeostasis through distinct metabolic mechanisms |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1008548&type=printable |
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