The Antinociceptive and Anti-Inflammatory Activities of Aspidosperma tomentosum (Apocynaceae)
We investigated the antinociceptive and anti-inflammatory activities of the crude ethanolic extract (CEE), its fractions, and the flavonoid isorhamnetin from Aspidosperma tomentosum using models of nociception and inflammation in mice. In the writhing test, the CEE and its fractions (except for solu...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/218627 |
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| author | Anansa Bezerra de Aquino Luiz Henrique Agra Cavalcante-Silva Carolina Barbosa Brito da Matta Willians Antônio do Nascimento Epifânio Pedro Gregório Vieira Aquino Antônio Euzébio Goulart Santana Magna Suzana Alexandre-Moreira João Xavier de Araújo-Júnior |
| author_facet | Anansa Bezerra de Aquino Luiz Henrique Agra Cavalcante-Silva Carolina Barbosa Brito da Matta Willians Antônio do Nascimento Epifânio Pedro Gregório Vieira Aquino Antônio Euzébio Goulart Santana Magna Suzana Alexandre-Moreira João Xavier de Araújo-Júnior |
| author_sort | Anansa Bezerra de Aquino |
| collection | DOAJ |
| description | We investigated the antinociceptive and anti-inflammatory activities of the crude ethanolic extract (CEE), its fractions, and the flavonoid isorhamnetin from Aspidosperma tomentosum using models of nociception and inflammation in mice. In the writhing test, the CEE and its fractions (except for soluble phase, CHCl3 100% and EtAcO 100%) at 100 mg/kg p.o. induced antinociceptive activity. Isorhamnetin (100 μmol/kg, p.o.) was also active. In the hot plate test, only the treatment with the fractions Hex : CHCl3 50%, CHCl3 100%, and CHCl3 : MeOH 5% (100 mg/kg, p.o.) increased the latency time, reversed by the opioid antagonist naloxone. Fractions that were active in the hot plate test did not show catalepsy condition. It was observed that CEE, all fractions, and isorhamnetin reduced the formalin effects in the neurogenic phase. In the inflammatory phase, only CEE, isorhamnetin, and CHCl3 100% and CHCl3 : MeOH 5% fractions were active. CEE and all fractions, except for CHCl3 : MeOH 10% fraction, isorhamnetin, and soluble fraction were able to produce an antioedematogenic activity in the ear capsaicin-induced edema test. In the thioglycolate-induced peritonitis, only EtAcO 100% fraction was not active. The results demonstrate that A. tomentosum has antinociceptive and anti-inflammatory activities in animal models. |
| format | Article |
| id | doaj-art-282c91a75ea04838aea38f04bf51b164 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-282c91a75ea04838aea38f04bf51b1642025-02-03T05:43:57ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/218627218627The Antinociceptive and Anti-Inflammatory Activities of Aspidosperma tomentosum (Apocynaceae)Anansa Bezerra de Aquino0Luiz Henrique Agra Cavalcante-Silva1Carolina Barbosa Brito da Matta2Willians Antônio do Nascimento Epifânio3Pedro Gregório Vieira Aquino4Antônio Euzébio Goulart Santana5Magna Suzana Alexandre-Moreira6João Xavier de Araújo-Júnior7LaFI—Laboratório de Farmacologia e Imunidade, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Maceió, AL, BrazilLaFI—Laboratório de Farmacologia e Imunidade, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Maceió, AL, BrazilLaFI—Laboratório de Farmacologia e Imunidade, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Maceió, AL, BrazilLaboratório de Pesquisa em Recursos Naturais, Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió, AL, BrazilLaboratório de Pesquisa em Recursos Naturais, Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió, AL, BrazilLaboratório de Pesquisa em Recursos Naturais, Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió, AL, BrazilLaFI—Laboratório de Farmacologia e Imunidade, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Maceió, AL, BrazilLaboratório de Pesquisa em Recursos Naturais, Instituto de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió, AL, BrazilWe investigated the antinociceptive and anti-inflammatory activities of the crude ethanolic extract (CEE), its fractions, and the flavonoid isorhamnetin from Aspidosperma tomentosum using models of nociception and inflammation in mice. In the writhing test, the CEE and its fractions (except for soluble phase, CHCl3 100% and EtAcO 100%) at 100 mg/kg p.o. induced antinociceptive activity. Isorhamnetin (100 μmol/kg, p.o.) was also active. In the hot plate test, only the treatment with the fractions Hex : CHCl3 50%, CHCl3 100%, and CHCl3 : MeOH 5% (100 mg/kg, p.o.) increased the latency time, reversed by the opioid antagonist naloxone. Fractions that were active in the hot plate test did not show catalepsy condition. It was observed that CEE, all fractions, and isorhamnetin reduced the formalin effects in the neurogenic phase. In the inflammatory phase, only CEE, isorhamnetin, and CHCl3 100% and CHCl3 : MeOH 5% fractions were active. CEE and all fractions, except for CHCl3 : MeOH 10% fraction, isorhamnetin, and soluble fraction were able to produce an antioedematogenic activity in the ear capsaicin-induced edema test. In the thioglycolate-induced peritonitis, only EtAcO 100% fraction was not active. The results demonstrate that A. tomentosum has antinociceptive and anti-inflammatory activities in animal models.http://dx.doi.org/10.1155/2013/218627 |
| spellingShingle | Anansa Bezerra de Aquino Luiz Henrique Agra Cavalcante-Silva Carolina Barbosa Brito da Matta Willians Antônio do Nascimento Epifânio Pedro Gregório Vieira Aquino Antônio Euzébio Goulart Santana Magna Suzana Alexandre-Moreira João Xavier de Araújo-Júnior The Antinociceptive and Anti-Inflammatory Activities of Aspidosperma tomentosum (Apocynaceae) The Scientific World Journal |
| title | The Antinociceptive and Anti-Inflammatory Activities of Aspidosperma tomentosum (Apocynaceae) |
| title_full | The Antinociceptive and Anti-Inflammatory Activities of Aspidosperma tomentosum (Apocynaceae) |
| title_fullStr | The Antinociceptive and Anti-Inflammatory Activities of Aspidosperma tomentosum (Apocynaceae) |
| title_full_unstemmed | The Antinociceptive and Anti-Inflammatory Activities of Aspidosperma tomentosum (Apocynaceae) |
| title_short | The Antinociceptive and Anti-Inflammatory Activities of Aspidosperma tomentosum (Apocynaceae) |
| title_sort | antinociceptive and anti inflammatory activities of aspidosperma tomentosum apocynaceae |
| url | http://dx.doi.org/10.1155/2013/218627 |
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