Effect of Th17 and Treg Axis Disorder on Outcomes of Pulmonary Arterial Hypertension in Connective Tissue Diseases

This prospective cohort study is to verify the hypothesis that the balance of Th17 and Treg cells frequencies in the peripheral circulation is disturbed in patients with varying degrees of connective tissue diseases-associated pulmonary arterial hypertension (CTD-aPAH) and to prove the influence of...

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Main Authors: Saren Gaowa, Wenyong Zhou, Lilei Yu, Xiaohui Zhou, Kai Liao, Kang Yang, Zhibin Lu, Hong Jiang, Xiaofeng Chen
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2014/247372
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author Saren Gaowa
Wenyong Zhou
Lilei Yu
Xiaohui Zhou
Kai Liao
Kang Yang
Zhibin Lu
Hong Jiang
Xiaofeng Chen
author_facet Saren Gaowa
Wenyong Zhou
Lilei Yu
Xiaohui Zhou
Kai Liao
Kang Yang
Zhibin Lu
Hong Jiang
Xiaofeng Chen
author_sort Saren Gaowa
collection DOAJ
description This prospective cohort study is to verify the hypothesis that the balance of Th17 and Treg cells frequencies in the peripheral circulation is disturbed in patients with varying degrees of connective tissue diseases-associated pulmonary arterial hypertension (CTD-aPAH) and to prove the influence of Th17/Treg imbalance on prognosis. We detected the frequencies and absolute counts of Th17 and Treg cells and related serum cytokines secretion and expressions of key transcription factors in 117 patients with connective tissue diseases (CTD), 53 patients with CTD-aPAH, and 48 healthy volunteers. Moreover, the median value according to levels of Th17/Treg ratios in patients with CTD-aPAH was chosen as basis of group division for survival analysis. CTD-aPAH patients revealed significant increase in peripheral Th17 cells, Th17-related cytokines, and ROR γt mRNA levels. They also presented a significant decrease in Treg cells, Treg-related cytokines, and Foxp3 mRNA levels as compared with CTD patients and healthy controls. More importantly, the Th17/Treg ratio was significantly related to the severity and prognosis of CTD-aPAH. This study indicated that the Th17/Treg axis disorder plays a critical role in CTD-aPAH. Furthermore, the dynamic balance between Th17 and Treg cells was likely to influence prognosis of patients with CTD-aPAH.
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spelling doaj-art-282aecc1e8a7415a8dac49fb6ed44a352025-08-20T02:04:17ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/247372247372Effect of Th17 and Treg Axis Disorder on Outcomes of Pulmonary Arterial Hypertension in Connective Tissue DiseasesSaren Gaowa0Wenyong Zhou1Lilei Yu2Xiaohui Zhou3Kai Liao4Kang Yang5Zhibin Lu6Hong Jiang7Xiaofeng Chen8Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Cardiothoracic Surgery, East Hospital, Tongji University, Shanghai 200120, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Cardiothoracic Surgery, East Hospital, Tongji University, Shanghai 200120, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Cardiothoracic Surgery, Huashan Hospital, Fudan University, Shanghai 200040, ChinaThis prospective cohort study is to verify the hypothesis that the balance of Th17 and Treg cells frequencies in the peripheral circulation is disturbed in patients with varying degrees of connective tissue diseases-associated pulmonary arterial hypertension (CTD-aPAH) and to prove the influence of Th17/Treg imbalance on prognosis. We detected the frequencies and absolute counts of Th17 and Treg cells and related serum cytokines secretion and expressions of key transcription factors in 117 patients with connective tissue diseases (CTD), 53 patients with CTD-aPAH, and 48 healthy volunteers. Moreover, the median value according to levels of Th17/Treg ratios in patients with CTD-aPAH was chosen as basis of group division for survival analysis. CTD-aPAH patients revealed significant increase in peripheral Th17 cells, Th17-related cytokines, and ROR γt mRNA levels. They also presented a significant decrease in Treg cells, Treg-related cytokines, and Foxp3 mRNA levels as compared with CTD patients and healthy controls. More importantly, the Th17/Treg ratio was significantly related to the severity and prognosis of CTD-aPAH. This study indicated that the Th17/Treg axis disorder plays a critical role in CTD-aPAH. Furthermore, the dynamic balance between Th17 and Treg cells was likely to influence prognosis of patients with CTD-aPAH.http://dx.doi.org/10.1155/2014/247372
spellingShingle Saren Gaowa
Wenyong Zhou
Lilei Yu
Xiaohui Zhou
Kai Liao
Kang Yang
Zhibin Lu
Hong Jiang
Xiaofeng Chen
Effect of Th17 and Treg Axis Disorder on Outcomes of Pulmonary Arterial Hypertension in Connective Tissue Diseases
Mediators of Inflammation
title Effect of Th17 and Treg Axis Disorder on Outcomes of Pulmonary Arterial Hypertension in Connective Tissue Diseases
title_full Effect of Th17 and Treg Axis Disorder on Outcomes of Pulmonary Arterial Hypertension in Connective Tissue Diseases
title_fullStr Effect of Th17 and Treg Axis Disorder on Outcomes of Pulmonary Arterial Hypertension in Connective Tissue Diseases
title_full_unstemmed Effect of Th17 and Treg Axis Disorder on Outcomes of Pulmonary Arterial Hypertension in Connective Tissue Diseases
title_short Effect of Th17 and Treg Axis Disorder on Outcomes of Pulmonary Arterial Hypertension in Connective Tissue Diseases
title_sort effect of th17 and treg axis disorder on outcomes of pulmonary arterial hypertension in connective tissue diseases
url http://dx.doi.org/10.1155/2014/247372
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