Melatonin Reduces Inflammatory Injury Through Inhibiting NF-κB Activation in Rats With Colitis
Proinflammatory mediators are important in the pathogenesis of IBD, which are regulated by activation of NF-κB. The aim of this study was to investigate whether melatonin reduces inflammatory injury and inhibits proinflammatory molecule and NF-κB in rats with colitis. Rat colitis model was establis...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2005-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI.2005.185 |
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| _version_ | 1832551383072505856 |
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| author | Jun-Hua Li Jie-Ping Yu Hong-Gang Yu Xi-Ming Xu Liang-Liang Yu Jin Liu He-Sheng Luo |
| author_facet | Jun-Hua Li Jie-Ping Yu Hong-Gang Yu Xi-Ming Xu Liang-Liang Yu Jin Liu He-Sheng Luo |
| author_sort | Jun-Hua Li |
| collection | DOAJ |
| description | Proinflammatory mediators are important in the pathogenesis of IBD, which are regulated by activation of NF-κB. The aim of this study was to investigate whether melatonin reduces inflammatory injury and inhibits proinflammatory molecule and NF-κB in rats with colitis. Rat colitis model was established by TNBS enema. NF-κB p65, TNF-α, ICAM-1, and IκBα in colon tissue were examined by immunohistochemistry, EMSA, RT-PCR, and Western blot analysis. Expression of proinflammatory molecule and activation of NF-κB were upregulated and IκB level decreased in rats with colitis. Melatonin reduces colonic inflammatory injury through downregulating proinflammatory molecule mediated by NF-κB inhibition and blockade of IκBα degradation. |
| format | Article |
| id | doaj-art-28285d2de3f9446e86dbffbd665e4849 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-28285d2de3f9446e86dbffbd665e48492025-02-03T06:01:31ZengWileyMediators of Inflammation0962-93511466-18612005-01-012005418519310.1155/MI.2005.185Melatonin Reduces Inflammatory Injury Through Inhibiting NF-κB Activation in Rats With ColitisJun-Hua Li0Jie-Ping Yu1Hong-Gang Yu2Xi-Ming Xu3Liang-Liang Yu4Jin Liu5He-Sheng Luo6Department of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Province, Wuhan 430060, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Province, Wuhan 430060, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Province, Wuhan 430060, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Province, Wuhan 430060, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Province, Wuhan 430060, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Province, Wuhan 430060, ChinaDepartment of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Province, Wuhan 430060, ChinaProinflammatory mediators are important in the pathogenesis of IBD, which are regulated by activation of NF-κB. The aim of this study was to investigate whether melatonin reduces inflammatory injury and inhibits proinflammatory molecule and NF-κB in rats with colitis. Rat colitis model was established by TNBS enema. NF-κB p65, TNF-α, ICAM-1, and IκBα in colon tissue were examined by immunohistochemistry, EMSA, RT-PCR, and Western blot analysis. Expression of proinflammatory molecule and activation of NF-κB were upregulated and IκB level decreased in rats with colitis. Melatonin reduces colonic inflammatory injury through downregulating proinflammatory molecule mediated by NF-κB inhibition and blockade of IκBα degradation.http://dx.doi.org/10.1155/MI.2005.185 |
| spellingShingle | Jun-Hua Li Jie-Ping Yu Hong-Gang Yu Xi-Ming Xu Liang-Liang Yu Jin Liu He-Sheng Luo Melatonin Reduces Inflammatory Injury Through Inhibiting NF-κB Activation in Rats With Colitis Mediators of Inflammation |
| title | Melatonin Reduces Inflammatory Injury Through Inhibiting NF-κB Activation in Rats With Colitis |
| title_full | Melatonin Reduces Inflammatory Injury Through Inhibiting NF-κB Activation in Rats With Colitis |
| title_fullStr | Melatonin Reduces Inflammatory Injury Through Inhibiting NF-κB Activation in Rats With Colitis |
| title_full_unstemmed | Melatonin Reduces Inflammatory Injury Through Inhibiting NF-κB Activation in Rats With Colitis |
| title_short | Melatonin Reduces Inflammatory Injury Through Inhibiting NF-κB Activation in Rats With Colitis |
| title_sort | melatonin reduces inflammatory injury through inhibiting nf κb activation in rats with colitis |
| url | http://dx.doi.org/10.1155/MI.2005.185 |
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