Enhanced Thermal and Storage Stability of Glucose Oxidase via Encapsulation in Chitosan-Coated Alginate and Carboxymethyl Cellulose Gel Particles
Glucose oxidase (GOD) is widely used as an important oxidoreductase in various fields. However, maintaining the vitality and stability of GOD under environmental stress is a challenge. To improve the thermal and storage stability of GOD, this study constructed sodium alginate–carboxymethyl cellulose...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/14/4/664 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850082197558525952 |
|---|---|
| author | Zhihao Guo Jian Ren Chunli Song |
| author_facet | Zhihao Guo Jian Ren Chunli Song |
| author_sort | Zhihao Guo |
| collection | DOAJ |
| description | Glucose oxidase (GOD) is widely used as an important oxidoreductase in various fields. However, maintaining the vitality and stability of GOD under environmental stress is a challenge. To improve the thermal and storage stability of GOD, this study constructed sodium alginate–carboxymethyl cellulose sodium gel particles (SA/CMC) and chitosan-coated SA/CMC gel particles (CS/SA/CMC) of GOD. The encapsulation efficiency (EE), gel particle structure, stability, and release behavior of GOD were evaluated. The results showed that the thermal stability of GOD encapsulated in SA/CMC and CS/SA/CMC gel particles was improved by approximately 2.8-fold and 4.3-fold compared with the free enzyme at 85 °C, respectively. In addition, CS/SA/CMC gel particles enhanced the enzyme activity retention rate of GOD to over 80% during storage at 4 °C for four weeks. Both SA/CMC and CS/SA/CMC gel particles loaded with GOD had more than 70% of the enzymes released during the simulated gastrointestinal experiment. The results demonstrated that encapsulating GOD in SA/CMC and CS/SA/CMC gel particles could improve its thermal stability and storage stability, which is conducive to further expanding the application of GOD in food, pharmaceutical and feed industries. |
| format | Article |
| id | doaj-art-282517dedea543f9abbd28c47c41452d |
| institution | DOAJ |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-282517dedea543f9abbd28c47c41452d2025-08-20T02:44:35ZengMDPI AGFoods2304-81582025-02-0114466410.3390/foods14040664Enhanced Thermal and Storage Stability of Glucose Oxidase via Encapsulation in Chitosan-Coated Alginate and Carboxymethyl Cellulose Gel ParticlesZhihao Guo0Jian Ren1Chunli Song2College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, ChinaCollege of Food and Bioengineering, Qiqihar University, Qiqihar 161006, ChinaCollege of Food and Bioengineering, Qiqihar University, Qiqihar 161006, ChinaGlucose oxidase (GOD) is widely used as an important oxidoreductase in various fields. However, maintaining the vitality and stability of GOD under environmental stress is a challenge. To improve the thermal and storage stability of GOD, this study constructed sodium alginate–carboxymethyl cellulose sodium gel particles (SA/CMC) and chitosan-coated SA/CMC gel particles (CS/SA/CMC) of GOD. The encapsulation efficiency (EE), gel particle structure, stability, and release behavior of GOD were evaluated. The results showed that the thermal stability of GOD encapsulated in SA/CMC and CS/SA/CMC gel particles was improved by approximately 2.8-fold and 4.3-fold compared with the free enzyme at 85 °C, respectively. In addition, CS/SA/CMC gel particles enhanced the enzyme activity retention rate of GOD to over 80% during storage at 4 °C for four weeks. Both SA/CMC and CS/SA/CMC gel particles loaded with GOD had more than 70% of the enzymes released during the simulated gastrointestinal experiment. The results demonstrated that encapsulating GOD in SA/CMC and CS/SA/CMC gel particles could improve its thermal stability and storage stability, which is conducive to further expanding the application of GOD in food, pharmaceutical and feed industries.https://www.mdpi.com/2304-8158/14/4/664glucose oxidasealginatesodium carboxymethyl cellulosechitosan coatingenzyme encapsulationthermal stability |
| spellingShingle | Zhihao Guo Jian Ren Chunli Song Enhanced Thermal and Storage Stability of Glucose Oxidase via Encapsulation in Chitosan-Coated Alginate and Carboxymethyl Cellulose Gel Particles Foods glucose oxidase alginate sodium carboxymethyl cellulose chitosan coating enzyme encapsulation thermal stability |
| title | Enhanced Thermal and Storage Stability of Glucose Oxidase via Encapsulation in Chitosan-Coated Alginate and Carboxymethyl Cellulose Gel Particles |
| title_full | Enhanced Thermal and Storage Stability of Glucose Oxidase via Encapsulation in Chitosan-Coated Alginate and Carboxymethyl Cellulose Gel Particles |
| title_fullStr | Enhanced Thermal and Storage Stability of Glucose Oxidase via Encapsulation in Chitosan-Coated Alginate and Carboxymethyl Cellulose Gel Particles |
| title_full_unstemmed | Enhanced Thermal and Storage Stability of Glucose Oxidase via Encapsulation in Chitosan-Coated Alginate and Carboxymethyl Cellulose Gel Particles |
| title_short | Enhanced Thermal and Storage Stability of Glucose Oxidase via Encapsulation in Chitosan-Coated Alginate and Carboxymethyl Cellulose Gel Particles |
| title_sort | enhanced thermal and storage stability of glucose oxidase via encapsulation in chitosan coated alginate and carboxymethyl cellulose gel particles |
| topic | glucose oxidase alginate sodium carboxymethyl cellulose chitosan coating enzyme encapsulation thermal stability |
| url | https://www.mdpi.com/2304-8158/14/4/664 |
| work_keys_str_mv | AT zhihaoguo enhancedthermalandstoragestabilityofglucoseoxidaseviaencapsulationinchitosancoatedalginateandcarboxymethylcellulosegelparticles AT jianren enhancedthermalandstoragestabilityofglucoseoxidaseviaencapsulationinchitosancoatedalginateandcarboxymethylcellulosegelparticles AT chunlisong enhancedthermalandstoragestabilityofglucoseoxidaseviaencapsulationinchitosancoatedalginateandcarboxymethylcellulosegelparticles |