Adherence and cytotoxicity of Acinetobacter baumannii on human cervical carcinoma epithelial cells: Exploring the role of anti-OmpA antibodies
We investigated the invasion of Acinetobacter baumannii strains to epithelial cells and elucidated the role of antibodies against outer membrane protein A (OmpA). A. baumannii ATCC 19606 and clinical isolate 58ST were utilized. OmpA was expressed, purified, and administered to BALB/c mice, inducing...
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Elsevier
2024-12-01
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| Series: | Medicine in Microecology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590097824000168 |
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| author | Reyhaneh Rafiei Delfan Zahra Fekrirad Mohammadreza Jalali Nadoushan Iraj Rasooli |
| author_facet | Reyhaneh Rafiei Delfan Zahra Fekrirad Mohammadreza Jalali Nadoushan Iraj Rasooli |
| author_sort | Reyhaneh Rafiei Delfan |
| collection | DOAJ |
| description | We investigated the invasion of Acinetobacter baumannii strains to epithelial cells and elucidated the role of antibodies against outer membrane protein A (OmpA). A. baumannii ATCC 19606 and clinical isolate 58ST were utilized. OmpA was expressed, purified, and administered to BALB/c mice, inducing anti-OmpA antibodies. OmpA cytotoxicity was evaluated. Two A. baumannii strains were selected to infect human cervical HeLa cells. Serum resistance was determined at sera dilutions. Adhesion, internalization, and proliferation of live and killed A. baumannii in HeLa cells were examined with and without anti-OmpA sera. HeLa cell viability was assessed with and without exposure of live A. baumannii strains to anti-OmpA sera. Cytoskeleton inhibitor experiments were conducted on epithelial cells to probe microfilament and microtubule involvement in A. baumannii invasion. OmpA prompted antibody production without toxicity in mice. A. baumannii strains displayed varying cell invasion abilities, notably the clinical strain exhibiting the highest invasion. A. baumannii cells localized within vacuoles during internalization, migrating towards the nucleus, using a zipper-like invasion process. Bacterial proliferation within host cells led to HeLa cell death. Pre-treatment with anti-OmpA antibodies significantly curbed adhesion and invasion of A. baumannii in HeLa cells. Microscopic imaging provided proof of the intracellular presence of A. baumannii in HeLa cells. In conclusion, the OmpA plays a crucial part in A. baumannii - epithelial cell interactions. The results add to our knowledge of pathogenesis during the initial stages of infection by A. baumannii. |
| format | Article |
| id | doaj-art-2824f3549ef0434da1fb8d3418b381cf |
| institution | OA Journals |
| issn | 2590-0978 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Medicine in Microecology |
| spelling | doaj-art-2824f3549ef0434da1fb8d3418b381cf2025-08-20T02:36:09ZengElsevierMedicine in Microecology2590-09782024-12-012210011310.1016/j.medmic.2024.100113Adherence and cytotoxicity of Acinetobacter baumannii on human cervical carcinoma epithelial cells: Exploring the role of anti-OmpA antibodiesReyhaneh Rafiei Delfan0Zahra Fekrirad1Mohammadreza Jalali Nadoushan2Iraj Rasooli3Department of Biology, Shahed University, Tehran, IranDepartment of Biology, Shahed University, Tehran, IranDepartment of Pathology, School of Medicine, Shahed University, Tehran, Iran; Department of Biology, and Molecular Microbiology Research Center of Shahed University, Tehran, IranDepartment of Biology, Shahed University, Tehran, Iran; Department of Biology, and Molecular Microbiology Research Center of Shahed University, Tehran, Iran; Corresponding author. Department of Biology, and Molecular Microbiology Research Center of Shahed University, Tehran, 3319118651, Iran.We investigated the invasion of Acinetobacter baumannii strains to epithelial cells and elucidated the role of antibodies against outer membrane protein A (OmpA). A. baumannii ATCC 19606 and clinical isolate 58ST were utilized. OmpA was expressed, purified, and administered to BALB/c mice, inducing anti-OmpA antibodies. OmpA cytotoxicity was evaluated. Two A. baumannii strains were selected to infect human cervical HeLa cells. Serum resistance was determined at sera dilutions. Adhesion, internalization, and proliferation of live and killed A. baumannii in HeLa cells were examined with and without anti-OmpA sera. HeLa cell viability was assessed with and without exposure of live A. baumannii strains to anti-OmpA sera. Cytoskeleton inhibitor experiments were conducted on epithelial cells to probe microfilament and microtubule involvement in A. baumannii invasion. OmpA prompted antibody production without toxicity in mice. A. baumannii strains displayed varying cell invasion abilities, notably the clinical strain exhibiting the highest invasion. A. baumannii cells localized within vacuoles during internalization, migrating towards the nucleus, using a zipper-like invasion process. Bacterial proliferation within host cells led to HeLa cell death. Pre-treatment with anti-OmpA antibodies significantly curbed adhesion and invasion of A. baumannii in HeLa cells. Microscopic imaging provided proof of the intracellular presence of A. baumannii in HeLa cells. In conclusion, the OmpA plays a crucial part in A. baumannii - epithelial cell interactions. The results add to our knowledge of pathogenesis during the initial stages of infection by A. baumannii.http://www.sciencedirect.com/science/article/pii/S2590097824000168Acinetobacter baumannii.Adherence.Internalization.Cytotoxicity.OmpA.HeLa |
| spellingShingle | Reyhaneh Rafiei Delfan Zahra Fekrirad Mohammadreza Jalali Nadoushan Iraj Rasooli Adherence and cytotoxicity of Acinetobacter baumannii on human cervical carcinoma epithelial cells: Exploring the role of anti-OmpA antibodies Medicine in Microecology Acinetobacter baumannii. Adherence. Internalization. Cytotoxicity. OmpA. HeLa |
| title | Adherence and cytotoxicity of Acinetobacter baumannii on human cervical carcinoma epithelial cells: Exploring the role of anti-OmpA antibodies |
| title_full | Adherence and cytotoxicity of Acinetobacter baumannii on human cervical carcinoma epithelial cells: Exploring the role of anti-OmpA antibodies |
| title_fullStr | Adherence and cytotoxicity of Acinetobacter baumannii on human cervical carcinoma epithelial cells: Exploring the role of anti-OmpA antibodies |
| title_full_unstemmed | Adherence and cytotoxicity of Acinetobacter baumannii on human cervical carcinoma epithelial cells: Exploring the role of anti-OmpA antibodies |
| title_short | Adherence and cytotoxicity of Acinetobacter baumannii on human cervical carcinoma epithelial cells: Exploring the role of anti-OmpA antibodies |
| title_sort | adherence and cytotoxicity of acinetobacter baumannii on human cervical carcinoma epithelial cells exploring the role of anti ompa antibodies |
| topic | Acinetobacter baumannii. Adherence. Internalization. Cytotoxicity. OmpA. HeLa |
| url | http://www.sciencedirect.com/science/article/pii/S2590097824000168 |
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