Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine
This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. The sponges were prepared by controlled...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2015/396131 |
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| _version_ | 1832563971224240128 |
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| author | Andréa Arruda Martins Shimojo Amanda Gomes Marcelino Perez Sofia Elisa Moraga Galdames Isabela Cambraia de Souza Brissac Maria Helena Andrade Santana |
| author_facet | Andréa Arruda Martins Shimojo Amanda Gomes Marcelino Perez Sofia Elisa Moraga Galdames Isabela Cambraia de Souza Brissac Maria Helena Andrade Santana |
| author_sort | Andréa Arruda Martins Shimojo |
| collection | DOAJ |
| description | This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. The sponges were prepared by controlled freezing (−20, −80, or −196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v). The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum. The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma. The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells. The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a −20°C freezing temperature, while −196°C favored osteogenic differentiation. Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days. |
| format | Article |
| id | doaj-art-282392081e8e43b99919e8ad23b93bd4 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-282392081e8e43b99919e8ad23b93bd42025-02-03T01:12:06ZengWileyThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/396131396131Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative MedicineAndréa Arruda Martins Shimojo0Amanda Gomes Marcelino Perez1Sofia Elisa Moraga Galdames2Isabela Cambraia de Souza Brissac3Maria Helena Andrade Santana4Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, UNICAMP, P.O. Box 6066, 13083-970 Campinas, SP, BrazilDepartment of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, UNICAMP, P.O. Box 6066, 13083-970 Campinas, SP, BrazilDepartment of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, UNICAMP, P.O. Box 6066, 13083-970 Campinas, SP, BrazilDepartment of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, UNICAMP, P.O. Box 6066, 13083-970 Campinas, SP, BrazilDepartment of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, UNICAMP, P.O. Box 6066, 13083-970 Campinas, SP, BrazilThis study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. The sponges were prepared by controlled freezing (−20, −80, or −196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v). The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum. The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma. The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells. The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a −20°C freezing temperature, while −196°C favored osteogenic differentiation. Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days.http://dx.doi.org/10.1155/2015/396131 |
| spellingShingle | Andréa Arruda Martins Shimojo Amanda Gomes Marcelino Perez Sofia Elisa Moraga Galdames Isabela Cambraia de Souza Brissac Maria Helena Andrade Santana Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine The Scientific World Journal |
| title | Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine |
| title_full | Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine |
| title_fullStr | Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine |
| title_full_unstemmed | Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine |
| title_short | Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine |
| title_sort | performance of prp associated with porous chitosan as a composite scaffold for regenerative medicine |
| url | http://dx.doi.org/10.1155/2015/396131 |
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