Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation
Objective(s): Cyclophosphamide (CP) is a chemotherapeutic drug used to treat various tumors. It causes nephrotoxicity by producing reactive oxygen species. Sinapic acid (SA) exhibits anti-oxidant, antiapoptotic, and anti-inflammatory activities at low doses as a phenylpropanoid. This study aimed to...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2025-05-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_25517_2fd79cc94edce4d6a599e9f50f3a60d4.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849730563266576384 |
|---|---|
| author | Saeed Raoof Shiva Rezaei Mehryar Zargari Masooreh Mirzaei Seyed Jalal Hosseinimehr Abbasali Karimpour Malekshah Fereshteh Talebpour Amiri |
| author_facet | Saeed Raoof Shiva Rezaei Mehryar Zargari Masooreh Mirzaei Seyed Jalal Hosseinimehr Abbasali Karimpour Malekshah Fereshteh Talebpour Amiri |
| author_sort | Saeed Raoof |
| collection | DOAJ |
| description | Objective(s): Cyclophosphamide (CP) is a chemotherapeutic drug used to treat various tumors. It causes nephrotoxicity by producing reactive oxygen species. Sinapic acid (SA) exhibits anti-oxidant, antiapoptotic, and anti-inflammatory activities at low doses as a phenylpropanoid. This study aimed to investigate the protective effects of SA on SP-induced renal injury. Materials and Methods: Forty-eight BALB/c mice were randomly divided into control, SA (for seven consecutive days, with two doses of 5 and 10 mg/kg), CP (single dose, 200 mg/kg), and CP + SA (5 and 10 mg/kg). On the 10th day of the study, mice were examined by renal function markers (Urea and Creatinine), oxidative stress markers (MDA and GSH), histopathological, and immunohistochemical assays (caspase-3 and NF-kB kidney). Results: MDA levels increased and GSH levels decreased significantly in CP-treated mice. In addition, the histopathological structure of the kidney tissue in CP-treated mice showed significantly severe kidney tissue damage associated with increased urea and creatinine. The administration of SA in CP-treated mice significantly reduced serum urea and creatinine concentrations. In addition, the immunohistochemical staining of caspase- 3 and NF-kB decreased significantly in the CP + SA group compared to CP-treated mice. Conclusion: Overall, our study suggests that sinapic acid, a substance with antioxidant, antiapoptotic, and anti-inflammatory properties, can be used as a complementary therapy to protect nephrotoxicity against CP. |
| format | Article |
| id | doaj-art-281c094c27504335b0e1f7694e36ffaa |
| institution | DOAJ |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-281c094c27504335b0e1f7694e36ffaa2025-08-20T03:08:50ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-05-0128565566110.22038/ijbms.2025.83903.1815525517Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluationSaeed Raoof0Shiva Rezaei1Mehryar Zargari2Masooreh Mirzaei3Seyed Jalal Hosseinimehr4Abbasali Karimpour Malekshah5Fereshteh Talebpour Amiri6Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, IranDepartment of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, IranDepartment of Biochemistry, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranDepartment of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, IranDepartment of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, IranDepartment of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, IranDepartment of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, IranObjective(s): Cyclophosphamide (CP) is a chemotherapeutic drug used to treat various tumors. It causes nephrotoxicity by producing reactive oxygen species. Sinapic acid (SA) exhibits anti-oxidant, antiapoptotic, and anti-inflammatory activities at low doses as a phenylpropanoid. This study aimed to investigate the protective effects of SA on SP-induced renal injury. Materials and Methods: Forty-eight BALB/c mice were randomly divided into control, SA (for seven consecutive days, with two doses of 5 and 10 mg/kg), CP (single dose, 200 mg/kg), and CP + SA (5 and 10 mg/kg). On the 10th day of the study, mice were examined by renal function markers (Urea and Creatinine), oxidative stress markers (MDA and GSH), histopathological, and immunohistochemical assays (caspase-3 and NF-kB kidney). Results: MDA levels increased and GSH levels decreased significantly in CP-treated mice. In addition, the histopathological structure of the kidney tissue in CP-treated mice showed significantly severe kidney tissue damage associated with increased urea and creatinine. The administration of SA in CP-treated mice significantly reduced serum urea and creatinine concentrations. In addition, the immunohistochemical staining of caspase- 3 and NF-kB decreased significantly in the CP + SA group compared to CP-treated mice. Conclusion: Overall, our study suggests that sinapic acid, a substance with antioxidant, antiapoptotic, and anti-inflammatory properties, can be used as a complementary therapy to protect nephrotoxicity against CP.https://ijbms.mums.ac.ir/article_25517_2fd79cc94edce4d6a599e9f50f3a60d4.pdfcaspase-3cyclophosphamidenephrotoxicitynf- kboxidative stresssinapic acid |
| spellingShingle | Saeed Raoof Shiva Rezaei Mehryar Zargari Masooreh Mirzaei Seyed Jalal Hosseinimehr Abbasali Karimpour Malekshah Fereshteh Talebpour Amiri Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation Iranian Journal of Basic Medical Sciences caspase-3 cyclophosphamide nephrotoxicity nf- kb oxidative stress sinapic acid |
| title | Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation |
| title_full | Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation |
| title_fullStr | Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation |
| title_full_unstemmed | Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation |
| title_short | Sinapic acid attenuated nephrotoxicity against Cyclophosphamide in mice model: A histochemical, immunohistochemical and histopathological evaluation |
| title_sort | sinapic acid attenuated nephrotoxicity against cyclophosphamide in mice model a histochemical immunohistochemical and histopathological evaluation |
| topic | caspase-3 cyclophosphamide nephrotoxicity nf- kb oxidative stress sinapic acid |
| url | https://ijbms.mums.ac.ir/article_25517_2fd79cc94edce4d6a599e9f50f3a60d4.pdf |
| work_keys_str_mv | AT saeedraoof sinapicacidattenuatednephrotoxicityagainstcyclophosphamideinmicemodelahistochemicalimmunohistochemicalandhistopathologicalevaluation AT shivarezaei sinapicacidattenuatednephrotoxicityagainstcyclophosphamideinmicemodelahistochemicalimmunohistochemicalandhistopathologicalevaluation AT mehryarzargari sinapicacidattenuatednephrotoxicityagainstcyclophosphamideinmicemodelahistochemicalimmunohistochemicalandhistopathologicalevaluation AT masoorehmirzaei sinapicacidattenuatednephrotoxicityagainstcyclophosphamideinmicemodelahistochemicalimmunohistochemicalandhistopathologicalevaluation AT seyedjalalhosseinimehr sinapicacidattenuatednephrotoxicityagainstcyclophosphamideinmicemodelahistochemicalimmunohistochemicalandhistopathologicalevaluation AT abbasalikarimpourmalekshah sinapicacidattenuatednephrotoxicityagainstcyclophosphamideinmicemodelahistochemicalimmunohistochemicalandhistopathologicalevaluation AT fereshtehtalebpouramiri sinapicacidattenuatednephrotoxicityagainstcyclophosphamideinmicemodelahistochemicalimmunohistochemicalandhistopathologicalevaluation |