Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing
In this study, decellularized fish skin (DFS) scaffold decorated with silver nanoparticles was prepared for accelerating burn wound healing. The silver nanoparticles (AgNPs) synthesized by the green and facile method using Aloe vera leaf at different incubating times were characterized by using X-ra...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2023/8541621 |
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| author | Surya Prasad Adhikari Astha Paudel Anisha Sharma Baruna Thapa Neha Khanal Nisha Shastri Sourav Rai Rameshwar Adhikari |
| author_facet | Surya Prasad Adhikari Astha Paudel Anisha Sharma Baruna Thapa Neha Khanal Nisha Shastri Sourav Rai Rameshwar Adhikari |
| author_sort | Surya Prasad Adhikari |
| collection | DOAJ |
| description | In this study, decellularized fish skin (DFS) scaffold decorated with silver nanoparticles was prepared for accelerating burn wound healing. The silver nanoparticles (AgNPs) synthesized by the green and facile method using Aloe vera leaf at different incubating times were characterized by using X-ray Diffraction (XRD), Fourier Transform Infrared (FT-IR) Spectroscopy, and Ultraviolet-Visible Spectroscopy (UV-Vis spectroscopy). The different characterizations confirmed that the sizes of AgNPs prepared by incubating for 6 hours and 12 hours were 29.1 nm and 35.2 nm, respectively. After that, the different concentrations of the smallest AgNPs were used to dope the DFS scaffold to determine the cell viability. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were used to correlate the concentration of AgNPs with its bactericidal effect which was seen from 50 μg/ml. Then, the toxicity with human cells was investigated using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay with no significant cell viability from the concentration of 50 μg/ml to 200 μg/ml compared to the cocultured and commercial treatments. |
| format | Article |
| id | doaj-art-281964ee9d014b1da231f58b2ac20b68 |
| institution | Kabale University |
| issn | 1687-8795 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-281964ee9d014b1da231f58b2ac20b682025-08-20T03:54:42ZengWileyInternational Journal of Biomaterials1687-87952023-01-01202310.1155/2023/8541621Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound HealingSurya Prasad Adhikari0Astha Paudel1Anisha Sharma2Baruna Thapa3Neha Khanal4Nisha Shastri5Sourav Rai6Rameshwar Adhikari7Department of Mechanical and Aerospace EngineeringCollege of Biomedical Engineering and Applied SciencesCollege of Biomedical Engineering and Applied SciencesCollege of Biomedical Engineering and Applied SciencesCollege of Biomedical Engineering and Applied SciencesCollege of Biomedical Engineering and Applied SciencesCollege of Biomedical Engineering and Applied SciencesCentre Department of ChemistryIn this study, decellularized fish skin (DFS) scaffold decorated with silver nanoparticles was prepared for accelerating burn wound healing. The silver nanoparticles (AgNPs) synthesized by the green and facile method using Aloe vera leaf at different incubating times were characterized by using X-ray Diffraction (XRD), Fourier Transform Infrared (FT-IR) Spectroscopy, and Ultraviolet-Visible Spectroscopy (UV-Vis spectroscopy). The different characterizations confirmed that the sizes of AgNPs prepared by incubating for 6 hours and 12 hours were 29.1 nm and 35.2 nm, respectively. After that, the different concentrations of the smallest AgNPs were used to dope the DFS scaffold to determine the cell viability. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were used to correlate the concentration of AgNPs with its bactericidal effect which was seen from 50 μg/ml. Then, the toxicity with human cells was investigated using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay with no significant cell viability from the concentration of 50 μg/ml to 200 μg/ml compared to the cocultured and commercial treatments.http://dx.doi.org/10.1155/2023/8541621 |
| spellingShingle | Surya Prasad Adhikari Astha Paudel Anisha Sharma Baruna Thapa Neha Khanal Nisha Shastri Sourav Rai Rameshwar Adhikari Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing International Journal of Biomaterials |
| title | Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing |
| title_full | Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing |
| title_fullStr | Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing |
| title_full_unstemmed | Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing |
| title_short | Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing |
| title_sort | development of decellularized fish skin scaffold decorated with biosynthesized silver nanoparticles for accelerated burn wound healing |
| url | http://dx.doi.org/10.1155/2023/8541621 |
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