Evaluation of common housefly Musca domestica maggot meal as partial substitution of fish meal and fish oil in Chinese mitten crab Eriocheir sinensis diets

The declining availability of fish meal (FM) and fish oil (FO) poses a challenge to the sustainable development of aquaculture, necessitating the exploration of nutritious and eco-friendly alternatives. This study evaluated the effects of partially replacing FM and FO with housefly Musca domestica m...

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Main Authors: Jinyu Tang, Yangxin Dai, Xiao Liang, Yu Zhang, Fuyong Huang, Bao Lou, Shuirong Guo
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Aquaculture Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S235251342500095X
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author Jinyu Tang
Yangxin Dai
Xiao Liang
Yu Zhang
Fuyong Huang
Bao Lou
Shuirong Guo
author_facet Jinyu Tang
Yangxin Dai
Xiao Liang
Yu Zhang
Fuyong Huang
Bao Lou
Shuirong Guo
author_sort Jinyu Tang
collection DOAJ
description The declining availability of fish meal (FM) and fish oil (FO) poses a challenge to the sustainable development of aquaculture, necessitating the exploration of nutritious and eco-friendly alternatives. This study evaluated the effects of partially replacing FM and FO with housefly Musca domestica maggot meal (HM) on the growth performance, nutritional composition, antioxidant capacity, and non-specific immune response of Chinese mitten crab Eriocheir sinensis. An 8-week feeding trial was conducted using six isonitrogenous (crude protein ≈ 42.0 %) and isolipidic (crude lipid ≈ 8.1 %) diets, with HM inclusion levels of 0, 54, 107, 161, 214, and 268 g/kg, corresponding to FM replacement levels of 0, 50, 100, 150, 200, and 250 g/kg, and FO replacement levels of 0, 4, 7, 11, 14, and 18 g/kg. A total of 240 crabs (120 males: 21.16 ± 4.09 g; 120 females: 21.04 ± 3.75 g) were randomly distributed into 30 tanks (4 crabs/tank; ♂:♀=1:1). Results showed that replacing FM and FO with up to 268 g/kg HM (71.4 % FM and 45 % FO) had no significant impact on growth performance or somatic index. Hepatic lipid, protein, essential amino acid, bitter amino acid and total amino acid increased with HM inclusion levels, while antioxidant capacity and lysozyme activity showed a quadratic relationship, peaking at specific HM inclusion levels before declining. Diets containing 214 g/kg and 268 g/kg HM promoted protein and lipid deposition in the hepatopancreas. Furthermore, HM inclusion levels of 161–268 g/kg significantly improved amino acid profiles in muscle and hepatopancreas, as well as fatty acid profiles in the hepatopancreas. HM supplementation also enhanced hepatic antioxidant capacity and lysozyme activity, with optimal effects observed at 132.22–228.77 g/kg. These findings demonstrate the potential of HM as a sustainable alternative to FM and FO in E. sinensis diets, with an optimal inclusion range of 132.22–228.77 g/kg.
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spelling doaj-art-280fe73febe44af2a5d9c1261b96b3d62025-08-20T03:05:55ZengElsevierAquaculture Reports2352-51342025-04-014110270910.1016/j.aqrep.2025.102709Evaluation of common housefly Musca domestica maggot meal as partial substitution of fish meal and fish oil in Chinese mitten crab Eriocheir sinensis dietsJinyu Tang0Yangxin Dai1Xiao Liang2Yu Zhang3Fuyong Huang4Bao Lou5Shuirong Guo6State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, ChinaHangzhou Academy of Agricultural Sciences, Hangzhou, Zhejiang 310024, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China; Corresponding authors.Hangzhou Center for Agricultural Technology Extension, Hangzhou, Zhejiang 310017, China; Corresponding authors.The declining availability of fish meal (FM) and fish oil (FO) poses a challenge to the sustainable development of aquaculture, necessitating the exploration of nutritious and eco-friendly alternatives. This study evaluated the effects of partially replacing FM and FO with housefly Musca domestica maggot meal (HM) on the growth performance, nutritional composition, antioxidant capacity, and non-specific immune response of Chinese mitten crab Eriocheir sinensis. An 8-week feeding trial was conducted using six isonitrogenous (crude protein ≈ 42.0 %) and isolipidic (crude lipid ≈ 8.1 %) diets, with HM inclusion levels of 0, 54, 107, 161, 214, and 268 g/kg, corresponding to FM replacement levels of 0, 50, 100, 150, 200, and 250 g/kg, and FO replacement levels of 0, 4, 7, 11, 14, and 18 g/kg. A total of 240 crabs (120 males: 21.16 ± 4.09 g; 120 females: 21.04 ± 3.75 g) were randomly distributed into 30 tanks (4 crabs/tank; ♂:♀=1:1). Results showed that replacing FM and FO with up to 268 g/kg HM (71.4 % FM and 45 % FO) had no significant impact on growth performance or somatic index. Hepatic lipid, protein, essential amino acid, bitter amino acid and total amino acid increased with HM inclusion levels, while antioxidant capacity and lysozyme activity showed a quadratic relationship, peaking at specific HM inclusion levels before declining. Diets containing 214 g/kg and 268 g/kg HM promoted protein and lipid deposition in the hepatopancreas. Furthermore, HM inclusion levels of 161–268 g/kg significantly improved amino acid profiles in muscle and hepatopancreas, as well as fatty acid profiles in the hepatopancreas. HM supplementation also enhanced hepatic antioxidant capacity and lysozyme activity, with optimal effects observed at 132.22–228.77 g/kg. These findings demonstrate the potential of HM as a sustainable alternative to FM and FO in E. sinensis diets, with an optimal inclusion range of 132.22–228.77 g/kg.http://www.sciencedirect.com/science/article/pii/S235251342500095XAntioxidant capacityEriocheir sinensisGrowth performanceMusca domesticaNutritional composition
spellingShingle Jinyu Tang
Yangxin Dai
Xiao Liang
Yu Zhang
Fuyong Huang
Bao Lou
Shuirong Guo
Evaluation of common housefly Musca domestica maggot meal as partial substitution of fish meal and fish oil in Chinese mitten crab Eriocheir sinensis diets
Aquaculture Reports
Antioxidant capacity
Eriocheir sinensis
Growth performance
Musca domestica
Nutritional composition
title Evaluation of common housefly Musca domestica maggot meal as partial substitution of fish meal and fish oil in Chinese mitten crab Eriocheir sinensis diets
title_full Evaluation of common housefly Musca domestica maggot meal as partial substitution of fish meal and fish oil in Chinese mitten crab Eriocheir sinensis diets
title_fullStr Evaluation of common housefly Musca domestica maggot meal as partial substitution of fish meal and fish oil in Chinese mitten crab Eriocheir sinensis diets
title_full_unstemmed Evaluation of common housefly Musca domestica maggot meal as partial substitution of fish meal and fish oil in Chinese mitten crab Eriocheir sinensis diets
title_short Evaluation of common housefly Musca domestica maggot meal as partial substitution of fish meal and fish oil in Chinese mitten crab Eriocheir sinensis diets
title_sort evaluation of common housefly musca domestica maggot meal as partial substitution of fish meal and fish oil in chinese mitten crab eriocheir sinensis diets
topic Antioxidant capacity
Eriocheir sinensis
Growth performance
Musca domestica
Nutritional composition
url http://www.sciencedirect.com/science/article/pii/S235251342500095X
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