LincRNA-EPS on intestinal flora of mice with periodontitis: An experimental study
[Objective:] To investigate the effect of long intergenic noncoding RNA‐EPS (lincRNA‐EPS) on intestinal flora in mice with periodontitis. [Methods:] 8‐week‐old male C57BL/6 wild‐type mice and lincRNA‐EPS knockout mice were selected and divided into wild‐type control group (group N, i.e. group 1, n=6...
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-06-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2023.03.002 |
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| author | CHEN Dongyi SU Jiansheng |
| author_facet | CHEN Dongyi SU Jiansheng |
| author_sort | CHEN Dongyi |
| collection | DOAJ |
| description | [Objective:] To investigate the effect of long intergenic noncoding RNA‐EPS (lincRNA‐EPS) on intestinal flora in mice with periodontitis. [Methods:] 8‐week‐old male C57BL/6 wild‐type mice and lincRNA‐EPS knockout mice were selected and divided into wild‐type control group (group N, i.e. group 1, n=6), knockout control group (group Nplus, i.e. group 2, n=6), wild‐type periodontitis group (group P, i.e. group 3, n=6), and knockout periodontitis group (group Pplus, i.e. group 4, n=5). The periodontitis model was established in mice group 3 and group 4 by silk ligation, and fecal samples were collected from each group after 10 d of ligation, then 16S rDNA sequencing was applied to analyze the composition and structure of the intestinal flora of each group. Micro‐CT was used to detect the morphology of the maxillary molar region in each group. [Results:] 16S rDNA sequencing showed that the lincRNA‐EPS knockout mice had no significant difference in the α diversity of intestinal flora compared with wild‐type periodontitis mice (P>0.05), and the β diversity was significantly different (P<0.05). The species composition and their abundance were significantly different at the phylum and genus levels (P<0.05). [Conclusion:] lincRNA‐EPS can change the composition of intestinal flora in mice with periodontitis to some extent. Further studies are required to develop conclusions regarding the performance. |
| format | Article |
| id | doaj-art-28068008de0140629c9a712bfad64a95 |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2023-06-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-28068008de0140629c9a712bfad64a952025-08-25T06:10:06ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792023-06-0133313814710.3969/j.issn.1005-4979.2023.03.002LincRNA-EPS on intestinal flora of mice with periodontitis: An experimental studyCHEN Dongyi0SU Jiansheng1Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China[Objective:] To investigate the effect of long intergenic noncoding RNA‐EPS (lincRNA‐EPS) on intestinal flora in mice with periodontitis. [Methods:] 8‐week‐old male C57BL/6 wild‐type mice and lincRNA‐EPS knockout mice were selected and divided into wild‐type control group (group N, i.e. group 1, n=6), knockout control group (group Nplus, i.e. group 2, n=6), wild‐type periodontitis group (group P, i.e. group 3, n=6), and knockout periodontitis group (group Pplus, i.e. group 4, n=5). The periodontitis model was established in mice group 3 and group 4 by silk ligation, and fecal samples were collected from each group after 10 d of ligation, then 16S rDNA sequencing was applied to analyze the composition and structure of the intestinal flora of each group. Micro‐CT was used to detect the morphology of the maxillary molar region in each group. [Results:] 16S rDNA sequencing showed that the lincRNA‐EPS knockout mice had no significant difference in the α diversity of intestinal flora compared with wild‐type periodontitis mice (P>0.05), and the β diversity was significantly different (P<0.05). The species composition and their abundance were significantly different at the phylum and genus levels (P<0.05). [Conclusion:] lincRNA‐EPS can change the composition of intestinal flora in mice with periodontitis to some extent. Further studies are required to develop conclusions regarding the performance.https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2023.03.002long intergenic noncoding rna‐epsperiodontitisintestinal florahigh‐throughput sequencing16s rdna |
| spellingShingle | CHEN Dongyi SU Jiansheng LincRNA-EPS on intestinal flora of mice with periodontitis: An experimental study Kouqiang hemian waike zazhi long intergenic noncoding rna‐eps periodontitis intestinal flora high‐throughput sequencing 16s rdna |
| title | LincRNA-EPS on intestinal flora of mice with periodontitis: An experimental study |
| title_full | LincRNA-EPS on intestinal flora of mice with periodontitis: An experimental study |
| title_fullStr | LincRNA-EPS on intestinal flora of mice with periodontitis: An experimental study |
| title_full_unstemmed | LincRNA-EPS on intestinal flora of mice with periodontitis: An experimental study |
| title_short | LincRNA-EPS on intestinal flora of mice with periodontitis: An experimental study |
| title_sort | lincrna eps on intestinal flora of mice with periodontitis an experimental study |
| topic | long intergenic noncoding rna‐eps periodontitis intestinal flora high‐throughput sequencing 16s rdna |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2023.03.002 |
| work_keys_str_mv | AT chendongyi lincrnaepsonintestinalfloraofmicewithperiodontitisanexperimentalstudy AT sujiansheng lincrnaepsonintestinalfloraofmicewithperiodontitisanexperimentalstudy |