Methylation and Expression of Retinoblastoma and Transforming Growth Factor-β1 Genes in Epstein-Barr Virus-Associated and -Negative Gastric Carcinomas
Background. Retinoblastoma (RB) and transforming growth factor-β1 (TGF-β1) are important tumor-related factors. Methods. A series of 30 EBV-associated gastric carcinoma (EBVaGC) and 38 matched EBV-negative gastric carcinoma (EBVnGC) tissues were examined for the promoter methylation of RB by methyla...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2012/906017 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564316734226432 |
|---|---|
| author | Xia Liu Xiuming Tang Shaoyan Zhang Yun Wang Xiaofeng Wang Chengquan Zhao Bing Luo |
| author_facet | Xia Liu Xiuming Tang Shaoyan Zhang Yun Wang Xiaofeng Wang Chengquan Zhao Bing Luo |
| author_sort | Xia Liu |
| collection | DOAJ |
| description | Background. Retinoblastoma (RB) and transforming growth factor-β1 (TGF-β1) are important tumor-related factors. Methods. A series of 30 EBV-associated gastric carcinoma (EBVaGC) and 38 matched EBV-negative gastric carcinoma (EBVnGC) tissues were examined for the promoter methylation of RB by methylation-specific PCR (MSP) method. The expression of RB and TGF-β1 in gastric carcinoma tissues was detected by immunohistochemistry. Results. The methylation rate of RB gene in EBVaGC and EBVnGC was 80.0% (24/30) and 50.0% (19/38), respectively. The difference of RB methylation rate between EBVaGC and EBVnGC was significant (χ2=6.490, P=0.011). There was no significant difference for RB expression between EBVaGC (43.3%, 13/30) and EBVnGC (63.2%, 24/38), and also for TGF-β1 between EBVaGC (56.7%, 17/30) and EBVnGC (63.2%, 24/38). RB methylation was not reversely correlated with RB expression in gastric carcinoma tissues (χ2=2.943, P=0.086, r=0.208). RB methylation, loss expression of RB, and TGF-β1 expression were significantly associated with tumor invasion and lymph node metastasis (P<0.05), but was not associated with sex, age, histological subtype (differentiation status) and tumor location. Conclusions. Methylation of RB is a common event in gastric carcinomas and EBV induces methylation of RB in EBVaGC, which may contribute to the development of gastric carcinomas. EBV has no significant effect on induction of TGF-β1 expression. Detection of RB methylation, RB expression, and TGF-β1 expression may be helpful to judge the status of tumor invasion and lymph node metastasis in gastric carcinomas. |
| format | Article |
| id | doaj-art-27fee58df3c947ca82f35787691e3a4a |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-27fee58df3c947ca82f35787691e3a4a2025-02-03T01:11:14ZengWileyGastroenterology Research and Practice1687-61211687-630X2012-01-01201210.1155/2012/906017906017Methylation and Expression of Retinoblastoma and Transforming Growth Factor-β1 Genes in Epstein-Barr Virus-Associated and -Negative Gastric CarcinomasXia Liu0Xiuming Tang1Shaoyan Zhang2Yun Wang3Xiaofeng Wang4Chengquan Zhao5Bing Luo6Department of Medical Microbiology, Qingdao University Medical College, 38 Dengzhou Road, Qingdao 266021, ChinaDepartment of Central Laboratory, The Affiliated Hospital of Qingdao University Medical College, 19 Jiangsu Road, Qingdao 266003, ChinaDepartment of Laboratory, The Affiliated Hospital of Qingdao University Medical College, Qingdao University, 19 Jiangsu Road, Qingdao 266003, ChinaDepartment of Medical Microbiology, Qingdao University Medical College, 38 Dengzhou Road, Qingdao 266021, ChinaDepartment of Medical Microbiology, Qingdao University Medical College, 38 Dengzhou Road, Qingdao 266021, ChinaDepartment of Pathology, University of Pittsburgh Medical Center, Pittsburgh PA 15213, USADepartment of Medical Microbiology, Qingdao University Medical College, 38 Dengzhou Road, Qingdao 266021, ChinaBackground. Retinoblastoma (RB) and transforming growth factor-β1 (TGF-β1) are important tumor-related factors. Methods. A series of 30 EBV-associated gastric carcinoma (EBVaGC) and 38 matched EBV-negative gastric carcinoma (EBVnGC) tissues were examined for the promoter methylation of RB by methylation-specific PCR (MSP) method. The expression of RB and TGF-β1 in gastric carcinoma tissues was detected by immunohistochemistry. Results. The methylation rate of RB gene in EBVaGC and EBVnGC was 80.0% (24/30) and 50.0% (19/38), respectively. The difference of RB methylation rate between EBVaGC and EBVnGC was significant (χ2=6.490, P=0.011). There was no significant difference for RB expression between EBVaGC (43.3%, 13/30) and EBVnGC (63.2%, 24/38), and also for TGF-β1 between EBVaGC (56.7%, 17/30) and EBVnGC (63.2%, 24/38). RB methylation was not reversely correlated with RB expression in gastric carcinoma tissues (χ2=2.943, P=0.086, r=0.208). RB methylation, loss expression of RB, and TGF-β1 expression were significantly associated with tumor invasion and lymph node metastasis (P<0.05), but was not associated with sex, age, histological subtype (differentiation status) and tumor location. Conclusions. Methylation of RB is a common event in gastric carcinomas and EBV induces methylation of RB in EBVaGC, which may contribute to the development of gastric carcinomas. EBV has no significant effect on induction of TGF-β1 expression. Detection of RB methylation, RB expression, and TGF-β1 expression may be helpful to judge the status of tumor invasion and lymph node metastasis in gastric carcinomas.http://dx.doi.org/10.1155/2012/906017 |
| spellingShingle | Xia Liu Xiuming Tang Shaoyan Zhang Yun Wang Xiaofeng Wang Chengquan Zhao Bing Luo Methylation and Expression of Retinoblastoma and Transforming Growth Factor-β1 Genes in Epstein-Barr Virus-Associated and -Negative Gastric Carcinomas Gastroenterology Research and Practice |
| title | Methylation and Expression of Retinoblastoma and Transforming Growth Factor-β1 Genes in Epstein-Barr Virus-Associated and -Negative Gastric Carcinomas |
| title_full | Methylation and Expression of Retinoblastoma and Transforming Growth Factor-β1 Genes in Epstein-Barr Virus-Associated and -Negative Gastric Carcinomas |
| title_fullStr | Methylation and Expression of Retinoblastoma and Transforming Growth Factor-β1 Genes in Epstein-Barr Virus-Associated and -Negative Gastric Carcinomas |
| title_full_unstemmed | Methylation and Expression of Retinoblastoma and Transforming Growth Factor-β1 Genes in Epstein-Barr Virus-Associated and -Negative Gastric Carcinomas |
| title_short | Methylation and Expression of Retinoblastoma and Transforming Growth Factor-β1 Genes in Epstein-Barr Virus-Associated and -Negative Gastric Carcinomas |
| title_sort | methylation and expression of retinoblastoma and transforming growth factor β1 genes in epstein barr virus associated and negative gastric carcinomas |
| url | http://dx.doi.org/10.1155/2012/906017 |
| work_keys_str_mv | AT xialiu methylationandexpressionofretinoblastomaandtransforminggrowthfactorb1genesinepsteinbarrvirusassociatedandnegativegastriccarcinomas AT xiumingtang methylationandexpressionofretinoblastomaandtransforminggrowthfactorb1genesinepsteinbarrvirusassociatedandnegativegastriccarcinomas AT shaoyanzhang methylationandexpressionofretinoblastomaandtransforminggrowthfactorb1genesinepsteinbarrvirusassociatedandnegativegastriccarcinomas AT yunwang methylationandexpressionofretinoblastomaandtransforminggrowthfactorb1genesinepsteinbarrvirusassociatedandnegativegastriccarcinomas AT xiaofengwang methylationandexpressionofretinoblastomaandtransforminggrowthfactorb1genesinepsteinbarrvirusassociatedandnegativegastriccarcinomas AT chengquanzhao methylationandexpressionofretinoblastomaandtransforminggrowthfactorb1genesinepsteinbarrvirusassociatedandnegativegastriccarcinomas AT bingluo methylationandexpressionofretinoblastomaandtransforminggrowthfactorb1genesinepsteinbarrvirusassociatedandnegativegastriccarcinomas |