An alternative approach to detect Trypanosoma in Glossina (Diptera, Glossinidae) without dissection
Background: Determining if a tsetse fly is infected by trypanosomes and thus potentially able to transmit trypanosome-related human and animal diseases is an extremely laborious and time-consuming task to perform, especially under field conditions. In this study we tested a possible alternative appr...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2008-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/324 |
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| author | Filipa Ferreira Jorge Cano Andreia Furtado Nicolas Ndong-Mabale Pedro Ndong-Asumu Agustin Benito João Pinto Maria Odete Afonso Jorge Seixas Jorge Atouguia Sónia Centeno-Lima |
| author_facet | Filipa Ferreira Jorge Cano Andreia Furtado Nicolas Ndong-Mabale Pedro Ndong-Asumu Agustin Benito João Pinto Maria Odete Afonso Jorge Seixas Jorge Atouguia Sónia Centeno-Lima |
| author_sort | Filipa Ferreira |
| collection | DOAJ |
| description | Background: Determining if a tsetse fly is infected by trypanosomes and thus potentially able to transmit trypanosome-related human and animal diseases is an extremely laborious and time-consuming task to perform, especially under field conditions. In this study we tested a possible alternative approach that uses the entire insect vector for DNA extraction and PCR analysis to detect and identify Trypanosoma spp. in field collected tsetse flies.
Methodology: DNA extraction was performed using a method originally developed for tick DNA extraction followed by PCR detection and identification of Trypanosoma spp.
Results: Two out of 62 flies captured in Equatorial Guinea carried DNA of T. brucei s.l. and Trypanosoma vivax. T. congolense forest, T. congolense savannah and T. congolense Kilifi were not detected.
Conclusions: The approach we employed allowed the molecular detection and species identification of trypanosomes using the whole vector body for DNA extraction. Although the approach does not give direct information on tsetse infectivity, it provides valuable information about trypanosome species circulating in a tsetse fly vector population. The method allows an effective processing of a large number of field captured tsetse in a central laboratory. |
| format | Article |
| id | doaj-art-27fdcd93c90f41cdaee212ecc030aaa5 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2008-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-27fdcd93c90f41cdaee212ecc030aaa52025-08-20T02:14:22ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802008-02-0120110.3855/jidc.324An alternative approach to detect Trypanosoma in Glossina (Diptera, Glossinidae) without dissectionFilipa Ferreira0Jorge Cano1Andreia Furtado2Nicolas Ndong-Mabale3Pedro Ndong-Asumu4Agustin Benito5João Pinto6Maria Odete Afonso7Jorge Seixas8Jorge Atouguia9Sónia Centeno-Lima10Unidade de Clínica das Doenças Tropicais and Centro de Malária e Outras Doenças Tropicais - LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa. Rua da Junqueira, 96, 1349-00 LisboaCentro Nacional de Medicina Tropical, Instituto de Salud Carlos III, Sinesio Delgado, 4 pabellón 13, 28029 MadridCentro de Malária e Outras Doenças Tropicais - LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa. Rua da Junqueira, 96, 1349-00 LisboaSleeping Sickness National Control Programme, BataSleeping Sickness National Control Programme, BataCentro Nacional de Medicina Tropical, Instituto de Salud Carlos III, Sinesio Delgado, 4 pabellón 13, 28029 MadridCentro de Malária e Outras Doenças Tropicais - LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa. Rua da Junqueira, 96, 1349-00 LisboaUnidade de Entomologia Médica/UPMM, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 96, 1349-006 LisboaUnidade de Clínica das Doenças Tropicais and Centro de Malária e Outras Doenças Tropicais - LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa. Rua da Junqueira, 96, 1349-00 LisboaUnidade de Clínica das Doenças Tropicais and Centro de Malária e Outras Doenças Tropicais - LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa. Rua da Junqueira, 96, 1349-00 LisboaUnidade de Clínica das Doenças Tropicais and Centro de Malária e Outras Doenças Tropicais - LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa. Rua da Junqueira, 96, 1349-00 LisboaBackground: Determining if a tsetse fly is infected by trypanosomes and thus potentially able to transmit trypanosome-related human and animal diseases is an extremely laborious and time-consuming task to perform, especially under field conditions. In this study we tested a possible alternative approach that uses the entire insect vector for DNA extraction and PCR analysis to detect and identify Trypanosoma spp. in field collected tsetse flies. Methodology: DNA extraction was performed using a method originally developed for tick DNA extraction followed by PCR detection and identification of Trypanosoma spp. Results: Two out of 62 flies captured in Equatorial Guinea carried DNA of T. brucei s.l. and Trypanosoma vivax. T. congolense forest, T. congolense savannah and T. congolense Kilifi were not detected. Conclusions: The approach we employed allowed the molecular detection and species identification of trypanosomes using the whole vector body for DNA extraction. Although the approach does not give direct information on tsetse infectivity, it provides valuable information about trypanosome species circulating in a tsetse fly vector population. The method allows an effective processing of a large number of field captured tsetse in a central laboratory.https://jidc.org/index.php/journal/article/view/324GlossinaTrypanosomaPCREquatorial Guinea |
| spellingShingle | Filipa Ferreira Jorge Cano Andreia Furtado Nicolas Ndong-Mabale Pedro Ndong-Asumu Agustin Benito João Pinto Maria Odete Afonso Jorge Seixas Jorge Atouguia Sónia Centeno-Lima An alternative approach to detect Trypanosoma in Glossina (Diptera, Glossinidae) without dissection Journal of Infection in Developing Countries Glossina Trypanosoma PCR Equatorial Guinea |
| title | An alternative approach to detect Trypanosoma in Glossina (Diptera, Glossinidae) without dissection |
| title_full | An alternative approach to detect Trypanosoma in Glossina (Diptera, Glossinidae) without dissection |
| title_fullStr | An alternative approach to detect Trypanosoma in Glossina (Diptera, Glossinidae) without dissection |
| title_full_unstemmed | An alternative approach to detect Trypanosoma in Glossina (Diptera, Glossinidae) without dissection |
| title_short | An alternative approach to detect Trypanosoma in Glossina (Diptera, Glossinidae) without dissection |
| title_sort | alternative approach to detect trypanosoma in glossina diptera glossinidae without dissection |
| topic | Glossina Trypanosoma PCR Equatorial Guinea |
| url | https://jidc.org/index.php/journal/article/view/324 |
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