A simple, cost-effective, and sustainable method for extraction and quantification of chebulagic acid from Terminalia bellirica (Gaertn.) Roxb. using RP-HPLC: A comparative study
Chebulagic acid (CA), a hydrolysable tannin found in Terminalia bellirica (T. bellirica) (Gaertn.) Roxb., has gained prominence as a significant component in Ayurvedic medicine. This study optimized methods for determining CA in T. bellirica, focusing on fruit samples and prioritizing simplicity, co...
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Elsevier
2024-12-01
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| Series: | Green Analytical Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S277257742400079X |
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| author | Nisha Nerlekar Aditya Magdum Vinod Nandre Mansingraj Nimbalkar Padma Dandge |
| author_facet | Nisha Nerlekar Aditya Magdum Vinod Nandre Mansingraj Nimbalkar Padma Dandge |
| author_sort | Nisha Nerlekar |
| collection | DOAJ |
| description | Chebulagic acid (CA), a hydrolysable tannin found in Terminalia bellirica (T. bellirica) (Gaertn.) Roxb., has gained prominence as a significant component in Ayurvedic medicine. This study optimized methods for determining CA in T. bellirica, focusing on fruit samples and prioritizing simplicity, cost-effectiveness, less time duration, and sustainability. We evaluated various extraction techniques, including continuous shaking, microwave assisted extraction, and Soxhlet extraction using green solvents. Quantification of CA was conducted using Reverse Phase High-Performance Liquid Chromatography (RP-HPLC), with confirmation achieved via UV–Visible (UV–Vis) spectroscopy, Thin Layer Chromatography (TLC), and Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS). Results indicated that Soxhlet and microwave significant extraction methods, particularly with ethanol as the green solvent. Soxhlet extraction yielded 49,607.73±18.82 μg/mL for fresh fruit and 42,952.53±19.09 μg/mL for dry fruit, while microwave extraction resulted in 43,627.56±113.48 μg/mL for fresh fruit and 40,466.33±130.90 μg/mL for dry fruit respectively. These findings conclude that ethanol as a green solvent in Soxhlet or microwave assisted extraction emerge as most efficient, straightforward, and effective approach for detecting and screening CA in T. bellirica samples. |
| format | Article |
| id | doaj-art-27f935eefa984205bc6c6dc2624cabb0 |
| institution | OA Journals |
| issn | 2772-5774 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Green Analytical Chemistry |
| spelling | doaj-art-27f935eefa984205bc6c6dc2624cabb02025-08-20T02:21:09ZengElsevierGreen Analytical Chemistry2772-57742024-12-011110017010.1016/j.greeac.2024.100170A simple, cost-effective, and sustainable method for extraction and quantification of chebulagic acid from Terminalia bellirica (Gaertn.) Roxb. using RP-HPLC: A comparative studyNisha Nerlekar0Aditya Magdum1Vinod Nandre2Mansingraj Nimbalkar3Padma Dandge4Department of Biochemistry, Shivaji University, Kolhapur, 416004, M.S., IndiaDepartment of Botany, Shivaji University, Kolhapur, 416004, M.S., IndiaSchool of Nanoscience and Technology, Shivaji University, Kolhapur, 416004, M.S. IndiaDepartment of Botany, Shivaji University, Kolhapur, 416004, M.S., IndiaDepartment of Biochemistry, Shivaji University, Kolhapur, 416004, M.S., India; Corresponding author.Chebulagic acid (CA), a hydrolysable tannin found in Terminalia bellirica (T. bellirica) (Gaertn.) Roxb., has gained prominence as a significant component in Ayurvedic medicine. This study optimized methods for determining CA in T. bellirica, focusing on fruit samples and prioritizing simplicity, cost-effectiveness, less time duration, and sustainability. We evaluated various extraction techniques, including continuous shaking, microwave assisted extraction, and Soxhlet extraction using green solvents. Quantification of CA was conducted using Reverse Phase High-Performance Liquid Chromatography (RP-HPLC), with confirmation achieved via UV–Visible (UV–Vis) spectroscopy, Thin Layer Chromatography (TLC), and Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS). Results indicated that Soxhlet and microwave significant extraction methods, particularly with ethanol as the green solvent. Soxhlet extraction yielded 49,607.73±18.82 μg/mL for fresh fruit and 42,952.53±19.09 μg/mL for dry fruit, while microwave extraction resulted in 43,627.56±113.48 μg/mL for fresh fruit and 40,466.33±130.90 μg/mL for dry fruit respectively. These findings conclude that ethanol as a green solvent in Soxhlet or microwave assisted extraction emerge as most efficient, straightforward, and effective approach for detecting and screening CA in T. bellirica samples.http://www.sciencedirect.com/science/article/pii/S277257742400079XT. bellirica (Gaertn.) Roxb.Plant extractChebulagic acidAnalytical methodsAntioxidant activityExtraction techniques etc |
| spellingShingle | Nisha Nerlekar Aditya Magdum Vinod Nandre Mansingraj Nimbalkar Padma Dandge A simple, cost-effective, and sustainable method for extraction and quantification of chebulagic acid from Terminalia bellirica (Gaertn.) Roxb. using RP-HPLC: A comparative study Green Analytical Chemistry T. bellirica (Gaertn.) Roxb. Plant extract Chebulagic acid Analytical methods Antioxidant activity Extraction techniques etc |
| title | A simple, cost-effective, and sustainable method for extraction and quantification of chebulagic acid from Terminalia bellirica (Gaertn.) Roxb. using RP-HPLC: A comparative study |
| title_full | A simple, cost-effective, and sustainable method for extraction and quantification of chebulagic acid from Terminalia bellirica (Gaertn.) Roxb. using RP-HPLC: A comparative study |
| title_fullStr | A simple, cost-effective, and sustainable method for extraction and quantification of chebulagic acid from Terminalia bellirica (Gaertn.) Roxb. using RP-HPLC: A comparative study |
| title_full_unstemmed | A simple, cost-effective, and sustainable method for extraction and quantification of chebulagic acid from Terminalia bellirica (Gaertn.) Roxb. using RP-HPLC: A comparative study |
| title_short | A simple, cost-effective, and sustainable method for extraction and quantification of chebulagic acid from Terminalia bellirica (Gaertn.) Roxb. using RP-HPLC: A comparative study |
| title_sort | simple cost effective and sustainable method for extraction and quantification of chebulagic acid from terminalia bellirica gaertn roxb using rp hplc a comparative study |
| topic | T. bellirica (Gaertn.) Roxb. Plant extract Chebulagic acid Analytical methods Antioxidant activity Extraction techniques etc |
| url | http://www.sciencedirect.com/science/article/pii/S277257742400079X |
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