Cordycepin Decreases Compound Action Potential Conduction of Frog Sciatic Nerve In Vitro Involving Ca2+-Dependent Mechanisms
Cordycepin has been widely used in oriental countries to maintain health and improve physical performance. Compound nerve action potential (CNAP), which is critical in signal conduction in the peripheral nervous system, is necessary to regulate physical performance, including motor system physiologi...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2015/927817 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553595355004928 |
|---|---|
| author | Li-Hua Yao Hui-Min Yu Qiu-Ping Xiong Wei Sun Yan-Liang Xu Wei Meng Yu-Ping Li Xin-Ping Liu Chun-Hua Yuan |
| author_facet | Li-Hua Yao Hui-Min Yu Qiu-Ping Xiong Wei Sun Yan-Liang Xu Wei Meng Yu-Ping Li Xin-Ping Liu Chun-Hua Yuan |
| author_sort | Li-Hua Yao |
| collection | DOAJ |
| description | Cordycepin has been widely used in oriental countries to maintain health and improve physical performance. Compound nerve action potential (CNAP), which is critical in signal conduction in the peripheral nervous system, is necessary to regulate physical performance, including motor system physiological and pathological processes. Therefore, regulatory effects of cordycepin on CNAP conduction should be elucidated. In this study, the conduction ability of CNAP in isolated frog sciatic nerves was investigated. Results revealed that cordycepin significantly decreased CNAP amplitude and conductive velocity in a reversible and concentration-dependent manner. At 50 mg/L cordycepin, CNAP amplitude and conductive velocity decreased by 62.18 ± 8.06% and 57.34% ± 6.14% compared with the control amplitude and conductive velocity, respectively. However, the depressive action of cordycepin on amplitude and conductive velocity was not observed in Ca2+-free medium or in the presence of Ca2+ channel blockers (CdCl2/LaCl3). Pretreatment with L-type Ca2+ channel antagonist (nifedipine/deltiazem) also blocked cordycepin-induced responses; by contrast, T-type and P-type Ca2+ channel antagonists (Ni2+) failed to block such responses. Therefore, cordycepin decreased the conduction ability of CNAP in isolated frog sciatic nerves via L-type Ca2+ channel-dependent mechanism. |
| format | Article |
| id | doaj-art-27f7f92cd3a041b4ba230dd8eb739bd3 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-27f7f92cd3a041b4ba230dd8eb739bd32025-02-03T05:53:39ZengWileyNeural Plasticity2090-59041687-54432015-01-01201510.1155/2015/927817927817Cordycepin Decreases Compound Action Potential Conduction of Frog Sciatic Nerve In Vitro Involving Ca2+-Dependent MechanismsLi-Hua Yao0Hui-Min Yu1Qiu-Ping Xiong2Wei Sun3Yan-Liang Xu4Wei Meng5Yu-Ping Li6Xin-Ping Liu7Chun-Hua Yuan8School of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, ChinaDepartment of Pathogenic Biology & Immunology, Medical College, Shenzhen University, Shenzhen, Guangdong 518060, ChinaInternal Medicine Department 3, Jiangxi Province Tumor Hospital, Nanchang, Jiangxi 330029, ChinaSchool of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, ChinaInternal Medicine Department 2, Jiangxi Province Tumor Hospital, Nanchang, Jiangxi 330029, ChinaSchool of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, ChinaSchool of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, ChinaSchool of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, ChinaSchool of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, ChinaCordycepin has been widely used in oriental countries to maintain health and improve physical performance. Compound nerve action potential (CNAP), which is critical in signal conduction in the peripheral nervous system, is necessary to regulate physical performance, including motor system physiological and pathological processes. Therefore, regulatory effects of cordycepin on CNAP conduction should be elucidated. In this study, the conduction ability of CNAP in isolated frog sciatic nerves was investigated. Results revealed that cordycepin significantly decreased CNAP amplitude and conductive velocity in a reversible and concentration-dependent manner. At 50 mg/L cordycepin, CNAP amplitude and conductive velocity decreased by 62.18 ± 8.06% and 57.34% ± 6.14% compared with the control amplitude and conductive velocity, respectively. However, the depressive action of cordycepin on amplitude and conductive velocity was not observed in Ca2+-free medium or in the presence of Ca2+ channel blockers (CdCl2/LaCl3). Pretreatment with L-type Ca2+ channel antagonist (nifedipine/deltiazem) also blocked cordycepin-induced responses; by contrast, T-type and P-type Ca2+ channel antagonists (Ni2+) failed to block such responses. Therefore, cordycepin decreased the conduction ability of CNAP in isolated frog sciatic nerves via L-type Ca2+ channel-dependent mechanism.http://dx.doi.org/10.1155/2015/927817 |
| spellingShingle | Li-Hua Yao Hui-Min Yu Qiu-Ping Xiong Wei Sun Yan-Liang Xu Wei Meng Yu-Ping Li Xin-Ping Liu Chun-Hua Yuan Cordycepin Decreases Compound Action Potential Conduction of Frog Sciatic Nerve In Vitro Involving Ca2+-Dependent Mechanisms Neural Plasticity |
| title | Cordycepin Decreases Compound Action Potential Conduction of Frog Sciatic Nerve In Vitro Involving Ca2+-Dependent Mechanisms |
| title_full | Cordycepin Decreases Compound Action Potential Conduction of Frog Sciatic Nerve In Vitro Involving Ca2+-Dependent Mechanisms |
| title_fullStr | Cordycepin Decreases Compound Action Potential Conduction of Frog Sciatic Nerve In Vitro Involving Ca2+-Dependent Mechanisms |
| title_full_unstemmed | Cordycepin Decreases Compound Action Potential Conduction of Frog Sciatic Nerve In Vitro Involving Ca2+-Dependent Mechanisms |
| title_short | Cordycepin Decreases Compound Action Potential Conduction of Frog Sciatic Nerve In Vitro Involving Ca2+-Dependent Mechanisms |
| title_sort | cordycepin decreases compound action potential conduction of frog sciatic nerve in vitro involving ca2 dependent mechanisms |
| url | http://dx.doi.org/10.1155/2015/927817 |
| work_keys_str_mv | AT lihuayao cordycepindecreasescompoundactionpotentialconductionoffrogsciaticnerveinvitroinvolvingca2dependentmechanisms AT huiminyu cordycepindecreasescompoundactionpotentialconductionoffrogsciaticnerveinvitroinvolvingca2dependentmechanisms AT qiupingxiong cordycepindecreasescompoundactionpotentialconductionoffrogsciaticnerveinvitroinvolvingca2dependentmechanisms AT weisun cordycepindecreasescompoundactionpotentialconductionoffrogsciaticnerveinvitroinvolvingca2dependentmechanisms AT yanliangxu cordycepindecreasescompoundactionpotentialconductionoffrogsciaticnerveinvitroinvolvingca2dependentmechanisms AT weimeng cordycepindecreasescompoundactionpotentialconductionoffrogsciaticnerveinvitroinvolvingca2dependentmechanisms AT yupingli cordycepindecreasescompoundactionpotentialconductionoffrogsciaticnerveinvitroinvolvingca2dependentmechanisms AT xinpingliu cordycepindecreasescompoundactionpotentialconductionoffrogsciaticnerveinvitroinvolvingca2dependentmechanisms AT chunhuayuan cordycepindecreasescompoundactionpotentialconductionoffrogsciaticnerveinvitroinvolvingca2dependentmechanisms |