Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters
Abstract Background Chickens play a crucial role as the primary global source of eggs and poultry, and the quality of rooster semen significantly impacts poultry reproductive efficiency. Therefore, it is imperative to comprehend the regulatory mechanisms underlying sperm development. Results In this...
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| Language: | English |
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BMC
2024-11-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-024-10836-8 |
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| author | Shihao Guo Bailin Cong Liyang Zhu Yao Zhang Ying Yang Xiaolong Qi Xiangguo Wang Longfei Xiao Cheng Long Yaxi Xu Xihui Sheng |
| author_facet | Shihao Guo Bailin Cong Liyang Zhu Yao Zhang Ying Yang Xiaolong Qi Xiangguo Wang Longfei Xiao Cheng Long Yaxi Xu Xihui Sheng |
| author_sort | Shihao Guo |
| collection | DOAJ |
| description | Abstract Background Chickens play a crucial role as the primary global source of eggs and poultry, and the quality of rooster semen significantly impacts poultry reproductive efficiency. Therefore, it is imperative to comprehend the regulatory mechanisms underlying sperm development. Results In this study, we established transcriptome profiles of lncRNAs, miRNAs, and mRNAs in 3 testis tissues and 3 epididymis tissues from “Jing Hong No.1” roosters at 24, 35, and 64 weeks of age. Using the data, we conducted whole transcriptome analysis and constructed a ceRNA network. We detected 10 differentially expressed mRNAs (DEmRNAs), 33 differentially expressed lncRNAs (DElncRNAs), and 10 differentially expressed miRNAs (DEmiRNAs) in the testis, as well as 149 DEmRNAs, 12 DElncRNAs, and 10 DEmiRNAs in the epididymis. These genes were found to be involved in cell differentiation and development, as well as various signaling pathways such as GnRH, MAPK, TGF-β, mTOR, VEGF, and calcium ion pathways. Subsequently, we constructed two competing endogenous RNA (ceRNA) networks comprising DEmRNAs, DElncRNAs, and DEmiRNAs. Furthermore, we identified four crucial lncRNA-mRNA-miRNA interactions that govern specific biological processes in the chicken reproductive system: MSTRG.2423.1-gga-miR-1563-PPP3CA and MSTRG.10064.2-gga-miR-32-5p-GPR12 regulating sperm motility in the testis; MSTRG.152556.1-gga-miR-9-3p-GREM1/THYN1 governing immunomodulation in the epididymis; and MSTRG.124708.1-gga-miR-375-NDUFB9/YBX1 controlling epididymal sperm maturation and motility. Conclusions Whole transcriptome sequencing of chicken testis and epididymis screened several key genes and ceRNA regulatory networks, which may be involved in the regulation of epididymal immunity, spermatogenesis and sperm viability through the pathways of MAPK, TGF-β, mTOR, and calcium ion. These findings contribute to our comprehensive understanding of the intricate molecular processes underlying rooster spermatogenesis, maturation and motility. |
| format | Article |
| id | doaj-art-27f7a0d9f9584771a9620032da92f8f0 |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-27f7a0d9f9584771a9620032da92f8f02024-11-10T12:09:06ZengBMCBMC Genomics1471-21642024-11-0125111510.1186/s12864-024-10836-8Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roostersShihao Guo0Bailin Cong1Liyang Zhu2Yao Zhang3Ying Yang4Xiaolong Qi5Xiangguo Wang6Longfei Xiao7Cheng Long8Yaxi Xu9Xihui Sheng10Animal Science and Technology College, Beijing University of AgricultureAnimal Science and Technology College, Beijing University of AgricultureAnimal Science and Technology College, Beijing University of AgricultureAnimal Science and Technology College, Beijing University of AgricultureAnimal Science and Technology College, Beijing University of AgricultureAnimal Science and Technology College, Beijing University of AgricultureAnimal Science and Technology College, Beijing University of AgricultureAnimal Science and Technology College, Beijing University of AgricultureAnimal Science and Technology College, Beijing University of AgricultureAnimal Science and Technology College, Beijing University of AgricultureAnimal Science and Technology College, Beijing University of AgricultureAbstract Background Chickens play a crucial role as the primary global source of eggs and poultry, and the quality of rooster semen significantly impacts poultry reproductive efficiency. Therefore, it is imperative to comprehend the regulatory mechanisms underlying sperm development. Results In this study, we established transcriptome profiles of lncRNAs, miRNAs, and mRNAs in 3 testis tissues and 3 epididymis tissues from “Jing Hong No.1” roosters at 24, 35, and 64 weeks of age. Using the data, we conducted whole transcriptome analysis and constructed a ceRNA network. We detected 10 differentially expressed mRNAs (DEmRNAs), 33 differentially expressed lncRNAs (DElncRNAs), and 10 differentially expressed miRNAs (DEmiRNAs) in the testis, as well as 149 DEmRNAs, 12 DElncRNAs, and 10 DEmiRNAs in the epididymis. These genes were found to be involved in cell differentiation and development, as well as various signaling pathways such as GnRH, MAPK, TGF-β, mTOR, VEGF, and calcium ion pathways. Subsequently, we constructed two competing endogenous RNA (ceRNA) networks comprising DEmRNAs, DElncRNAs, and DEmiRNAs. Furthermore, we identified four crucial lncRNA-mRNA-miRNA interactions that govern specific biological processes in the chicken reproductive system: MSTRG.2423.1-gga-miR-1563-PPP3CA and MSTRG.10064.2-gga-miR-32-5p-GPR12 regulating sperm motility in the testis; MSTRG.152556.1-gga-miR-9-3p-GREM1/THYN1 governing immunomodulation in the epididymis; and MSTRG.124708.1-gga-miR-375-NDUFB9/YBX1 controlling epididymal sperm maturation and motility. Conclusions Whole transcriptome sequencing of chicken testis and epididymis screened several key genes and ceRNA regulatory networks, which may be involved in the regulation of epididymal immunity, spermatogenesis and sperm viability through the pathways of MAPK, TGF-β, mTOR, and calcium ion. These findings contribute to our comprehensive understanding of the intricate molecular processes underlying rooster spermatogenesis, maturation and motility.https://doi.org/10.1186/s12864-024-10836-8RoosterTestisEpididymisceRNAWhole transcriptome |
| spellingShingle | Shihao Guo Bailin Cong Liyang Zhu Yao Zhang Ying Yang Xiaolong Qi Xiangguo Wang Longfei Xiao Cheng Long Yaxi Xu Xihui Sheng Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters BMC Genomics Rooster Testis Epididymis ceRNA Whole transcriptome |
| title | Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters |
| title_full | Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters |
| title_fullStr | Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters |
| title_full_unstemmed | Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters |
| title_short | Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters |
| title_sort | whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters |
| topic | Rooster Testis Epididymis ceRNA Whole transcriptome |
| url | https://doi.org/10.1186/s12864-024-10836-8 |
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