Potential protective regulatory effects on radiation−induced esophageal injury in TUT4−/− mice
IntroductionTerminal uridyl transferase 4 (TUT4), a nucleotide transferase that modifies miRNA sequences, plays a critical role in regulating miRNA target interactions and function. However, its involvement in radiation-induced esophageal injury remains poorly understood.MethodsTo investigate this,...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2025.1600597/full |
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| author | Huiwen Ren Wei Li Zhigang Fan Jianlin Wang Zhiqiang Sun Renhua Huang Judong Luo Bo Gao |
| author_facet | Huiwen Ren Wei Li Zhigang Fan Jianlin Wang Zhiqiang Sun Renhua Huang Judong Luo Bo Gao |
| author_sort | Huiwen Ren |
| collection | DOAJ |
| description | IntroductionTerminal uridyl transferase 4 (TUT4), a nucleotide transferase that modifies miRNA sequences, plays a critical role in regulating miRNA target interactions and function. However, its involvement in radiation-induced esophageal injury remains poorly understood.MethodsTo investigate this, we performed computational analysis of RNA-seq data from irradiated esophageal tissues of wild-type and TUT4-knockout (TUT4–/–) mice, identifying 53 differentially expressed mRNAs (DEmRNAs), of which 30 were upregulated and 23 downregulated.ResultsGene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that these DEmRNAs were significantly associated with biological processes including lipid metabolism, fatty acid metabolism, proteolysis, and broader metabolic functions. Notably, DEmRNAs in TUT4–/– esophageal tissues showed marked enrichment in the renin–angiotensin system and peroxisome proliferator-activated receptor signaling pathways, implicating their potential roles in the pathogenesis of radiation-induced esophageal injury. In addition, we identified a regulatory axis in which a long non-coding RNA competes with miR-182 to modulate the competing endogenous RNA network governing TUT4 target genes. Collectively, our transcriptomic analysis offers novel mechanistic insights into how TUT4 may confer protection against radiation-induced damage in esophageal tissues. |
| format | Article |
| id | doaj-art-27efa69bd00a4a108124e2b608f1150a |
| institution | Kabale University |
| issn | 2234-943X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj-art-27efa69bd00a4a108124e2b608f1150a2025-08-26T04:12:43ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2025-08-011510.3389/fonc.2025.16005971600597Potential protective regulatory effects on radiation−induced esophageal injury in TUT4−/− miceHuiwen Ren0Wei Li1Zhigang Fan2Jianlin Wang3Zhiqiang Sun4Renhua Huang5Judong Luo6Bo Gao7Department of Radiotherapy, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaClinical Nutrition Department of The Tenth People’s Hospital, Tongji University, Shanghai, ChinaDepartment of Oncology, 3201 Hospital of Xi’an Jiaotong University Health Science Center, Shanghai, ChinaDepartment of Radiotherapy, The Second People’s Hospital of Changzhou, the Third Affiliated Hospital of Nanjing Medical University, Changzhou, ChinaDepartment of Radiotherapy, The Second People’s Hospital of Changzhou, the Third Affiliated Hospital of Nanjing Medical University, Changzhou, ChinaDepartment of Radiation, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Radiotherapy, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Rheumatology and Immunology, The Second People's Hospital of Changzhou, the Third Affilitated Hosptial of Nanjing Medical University, Changzhou, ChinaIntroductionTerminal uridyl transferase 4 (TUT4), a nucleotide transferase that modifies miRNA sequences, plays a critical role in regulating miRNA target interactions and function. However, its involvement in radiation-induced esophageal injury remains poorly understood.MethodsTo investigate this, we performed computational analysis of RNA-seq data from irradiated esophageal tissues of wild-type and TUT4-knockout (TUT4–/–) mice, identifying 53 differentially expressed mRNAs (DEmRNAs), of which 30 were upregulated and 23 downregulated.ResultsGene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that these DEmRNAs were significantly associated with biological processes including lipid metabolism, fatty acid metabolism, proteolysis, and broader metabolic functions. Notably, DEmRNAs in TUT4–/– esophageal tissues showed marked enrichment in the renin–angiotensin system and peroxisome proliferator-activated receptor signaling pathways, implicating their potential roles in the pathogenesis of radiation-induced esophageal injury. In addition, we identified a regulatory axis in which a long non-coding RNA competes with miR-182 to modulate the competing endogenous RNA network governing TUT4 target genes. Collectively, our transcriptomic analysis offers novel mechanistic insights into how TUT4 may confer protection against radiation-induced damage in esophageal tissues.https://www.frontiersin.org/articles/10.3389/fonc.2025.1600597/fullesophageal tissueradioprotectionTUT4radiation-induced esophageal injuryradioactivity |
| spellingShingle | Huiwen Ren Wei Li Zhigang Fan Jianlin Wang Zhiqiang Sun Renhua Huang Judong Luo Bo Gao Potential protective regulatory effects on radiation−induced esophageal injury in TUT4−/− mice Frontiers in Oncology esophageal tissue radioprotection TUT4 radiation-induced esophageal injury radioactivity |
| title | Potential protective regulatory effects on radiation−induced esophageal injury in TUT4−/− mice |
| title_full | Potential protective regulatory effects on radiation−induced esophageal injury in TUT4−/− mice |
| title_fullStr | Potential protective regulatory effects on radiation−induced esophageal injury in TUT4−/− mice |
| title_full_unstemmed | Potential protective regulatory effects on radiation−induced esophageal injury in TUT4−/− mice |
| title_short | Potential protective regulatory effects on radiation−induced esophageal injury in TUT4−/− mice |
| title_sort | potential protective regulatory effects on radiation induced esophageal injury in tut4 mice |
| topic | esophageal tissue radioprotection TUT4 radiation-induced esophageal injury radioactivity |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2025.1600597/full |
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