Investigation of heteroresistant vancomycin intermediate Staphylococcus aureus among MRSA isolates
Introduction: Heteroresistant vancomycin intermediate Staphylococcus aureus (hVISA) testing is recommended when therapeutic failure is suspected in the clinics. In our research, we aimed to investigate the prevalence of hVISA among methicilline-resistant S. aureus (MRSA) isolates in our university...
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The Journal of Infection in Developing Countries
2021-01-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/12799 |
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| author | Deniz Gazel Mehmet Erinmez Ayşe Büyüktaş Manay Yasemin Zer |
| author_facet | Deniz Gazel Mehmet Erinmez Ayşe Büyüktaş Manay Yasemin Zer |
| author_sort | Deniz Gazel |
| collection | DOAJ |
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Introduction: Heteroresistant vancomycin intermediate Staphylococcus aureus (hVISA) testing is recommended when therapeutic failure is suspected in the clinics. In our research, we aimed to investigate the prevalence of hVISA among methicilline-resistant S. aureus (MRSA) isolates in our university hospital and compared three methods for detection of hVISA.
Methodology: One hundred MRSA clinical isolates were collected in our medical microbiology laboratory between 01.04.2018 and 01.10.2019. For screening of hVISA, we used two screening agar plates and used one commercial medium; brain heart infusion agar (BHI) plates containing 4 µg/mL vancomycin and 16 g/Lt casein (BHIA-VC; Satola’s test), BHI agar plates containing 4 µg/mLvancomycin (BHIAV), and commercially obtained vancomycin resistant Enterococci (VRE) agar for detetection of hVISA. Colonies which could grow on plates were counted manually at 24th and 48th hours.
Results: Among 100 MRSA isolates, 43 (43%) were found as hVISA using Satola’s test. BHIAV and VRE agar screening test results were found 70% and 4%, respectively. Finally, at the step, MIC values of 20 (47%) hVISA isolates reduced to 2 µg/mL after sub culturing for the gradient test.
Conclusions: We found higher rates of hVISA comparing other studies in Turkey. Both VRE agar and BHIAV screening test failed to detect hVISA properly. Meropenem in combination with vancomycin inhibited the growth of 90% hVISA isolates in our study.
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| format | Article |
| id | doaj-art-27e8eaaaf394436bb6d5ced66f708741 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-27e8eaaaf394436bb6d5ced66f7087412025-08-20T02:57:13ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802021-01-01150110.3855/jidc.12799Investigation of heteroresistant vancomycin intermediate Staphylococcus aureus among MRSA isolatesDeniz Gazel0Mehmet Erinmez1Ayşe Büyüktaş Manay2Yasemin Zer3Department of Medical Microbiology, School of Medicine, Gaziantep University, Gaziantep, TurkeyDepartment of Medical Microbiology, School of Medicine, Gaziantep University, Gaziantep, TurkeyDepartment of Medical Microbiology, School of Medicine, Gaziantep University, Gaziantep, TurkeyDepartment of Medical Microbiology, School of Medicine, Gaziantep University, Gaziantep, Turkey Introduction: Heteroresistant vancomycin intermediate Staphylococcus aureus (hVISA) testing is recommended when therapeutic failure is suspected in the clinics. In our research, we aimed to investigate the prevalence of hVISA among methicilline-resistant S. aureus (MRSA) isolates in our university hospital and compared three methods for detection of hVISA. Methodology: One hundred MRSA clinical isolates were collected in our medical microbiology laboratory between 01.04.2018 and 01.10.2019. For screening of hVISA, we used two screening agar plates and used one commercial medium; brain heart infusion agar (BHI) plates containing 4 µg/mL vancomycin and 16 g/Lt casein (BHIA-VC; Satola’s test), BHI agar plates containing 4 µg/mLvancomycin (BHIAV), and commercially obtained vancomycin resistant Enterococci (VRE) agar for detetection of hVISA. Colonies which could grow on plates were counted manually at 24th and 48th hours. Results: Among 100 MRSA isolates, 43 (43%) were found as hVISA using Satola’s test. BHIAV and VRE agar screening test results were found 70% and 4%, respectively. Finally, at the step, MIC values of 20 (47%) hVISA isolates reduced to 2 µg/mL after sub culturing for the gradient test. Conclusions: We found higher rates of hVISA comparing other studies in Turkey. Both VRE agar and BHIAV screening test failed to detect hVISA properly. Meropenem in combination with vancomycin inhibited the growth of 90% hVISA isolates in our study. https://jidc.org/index.php/journal/article/view/12799S. aureusMRSAhVISAscreeningVRE agar |
| spellingShingle | Deniz Gazel Mehmet Erinmez Ayşe Büyüktaş Manay Yasemin Zer Investigation of heteroresistant vancomycin intermediate Staphylococcus aureus among MRSA isolates Journal of Infection in Developing Countries S. aureus MRSA hVISA screening VRE agar |
| title | Investigation of heteroresistant vancomycin intermediate Staphylococcus aureus among MRSA isolates |
| title_full | Investigation of heteroresistant vancomycin intermediate Staphylococcus aureus among MRSA isolates |
| title_fullStr | Investigation of heteroresistant vancomycin intermediate Staphylococcus aureus among MRSA isolates |
| title_full_unstemmed | Investigation of heteroresistant vancomycin intermediate Staphylococcus aureus among MRSA isolates |
| title_short | Investigation of heteroresistant vancomycin intermediate Staphylococcus aureus among MRSA isolates |
| title_sort | investigation of heteroresistant vancomycin intermediate staphylococcus aureus among mrsa isolates |
| topic | S. aureus MRSA hVISA screening VRE agar |
| url | https://jidc.org/index.php/journal/article/view/12799 |
| work_keys_str_mv | AT denizgazel investigationofheteroresistantvancomycinintermediatestaphylococcusaureusamongmrsaisolates AT mehmeterinmez investigationofheteroresistantvancomycinintermediatestaphylococcusaureusamongmrsaisolates AT aysebuyuktasmanay investigationofheteroresistantvancomycinintermediatestaphylococcusaureusamongmrsaisolates AT yaseminzer investigationofheteroresistantvancomycinintermediatestaphylococcusaureusamongmrsaisolates |