Determination of 14 Human Milk Oligosaccharides in Infant Formula Milk Powder by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry
A method for the determination of 14 human milk oligosaccharides (HMOs) in infant formula milk powder was established by hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The sample was deproteinized by ethanol precipitation and degreased by freezing prior to sepa...
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China Food Publishing Company
2025-02-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-026.pdf |
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| author | YE Mengwei, HUANG Qian, FENG Lifeng, HUANG Yuan, CAI Xiaoming, GAO Yu, WANG Tianxi, PAN Cheng |
| author_facet | YE Mengwei, HUANG Qian, FENG Lifeng, HUANG Yuan, CAI Xiaoming, GAO Yu, WANG Tianxi, PAN Cheng |
| author_sort | YE Mengwei, HUANG Qian, FENG Lifeng, HUANG Yuan, CAI Xiaoming, GAO Yu, WANG Tianxi, PAN Cheng |
| collection | DOAJ |
| description | A method for the determination of 14 human milk oligosaccharides (HMOs) in infant formula milk powder was established by hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The sample was deproteinized by ethanol precipitation and degreased by freezing prior to separation by HILIC. The analytes were detected by multiple reaction monitoring (MRM) and quantified by an external standard method. Under optimized conditions, all HMOs showed good linear relationships within their respective concentration ranges, with determination coefficients (R2) greater than 0.994. The average recovery rates from blank goat’s and cow’s milk powders at three spiked levels ranged from 90.3% to 109.2%, with relative standard deviation (RSD) in the range of 0.7%–9.7% (n = 6), and the limit of detection (LOD) between 0.002 and 0.048 μg/mL. The developed method is characterized by fast analysis speed, high sensitivity and good accuracy, and meets the requirements for the rapid detection of the 14 HMOs in infant formula milk powder. |
| format | Article |
| id | doaj-art-27d75bc14dfb44e1a42fbca2dab6ef94 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-27d75bc14dfb44e1a42fbca2dab6ef942025-02-05T09:08:33ZengChina Food Publishing CompanyShipin Kexue1002-66302025-02-0146323023710.7506/spkx1002-6630-20240409-072Determination of 14 Human Milk Oligosaccharides in Infant Formula Milk Powder by Hydrophilic Interaction Liquid Chromatography-Tandem Mass SpectrometryYE Mengwei, HUANG Qian, FENG Lifeng, HUANG Yuan, CAI Xiaoming, GAO Yu, WANG Tianxi, PAN Cheng0(National Quality Supervision and Testing Center for Processed Food, Fujian Inspection and Research Institute for Product Quality, Fuzhou 350002, China)A method for the determination of 14 human milk oligosaccharides (HMOs) in infant formula milk powder was established by hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The sample was deproteinized by ethanol precipitation and degreased by freezing prior to separation by HILIC. The analytes were detected by multiple reaction monitoring (MRM) and quantified by an external standard method. Under optimized conditions, all HMOs showed good linear relationships within their respective concentration ranges, with determination coefficients (R2) greater than 0.994. The average recovery rates from blank goat’s and cow’s milk powders at three spiked levels ranged from 90.3% to 109.2%, with relative standard deviation (RSD) in the range of 0.7%–9.7% (n = 6), and the limit of detection (LOD) between 0.002 and 0.048 μg/mL. The developed method is characterized by fast analysis speed, high sensitivity and good accuracy, and meets the requirements for the rapid detection of the 14 HMOs in infant formula milk powder.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-026.pdfinfant formula milk powder; human milk oligosaccharides; hydrophilic interaction liquid chromatography-tandem mass spectrometry |
| spellingShingle | YE Mengwei, HUANG Qian, FENG Lifeng, HUANG Yuan, CAI Xiaoming, GAO Yu, WANG Tianxi, PAN Cheng Determination of 14 Human Milk Oligosaccharides in Infant Formula Milk Powder by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry Shipin Kexue infant formula milk powder; human milk oligosaccharides; hydrophilic interaction liquid chromatography-tandem mass spectrometry |
| title | Determination of 14 Human Milk Oligosaccharides in Infant Formula Milk Powder by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry |
| title_full | Determination of 14 Human Milk Oligosaccharides in Infant Formula Milk Powder by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry |
| title_fullStr | Determination of 14 Human Milk Oligosaccharides in Infant Formula Milk Powder by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry |
| title_full_unstemmed | Determination of 14 Human Milk Oligosaccharides in Infant Formula Milk Powder by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry |
| title_short | Determination of 14 Human Milk Oligosaccharides in Infant Formula Milk Powder by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry |
| title_sort | determination of 14 human milk oligosaccharides in infant formula milk powder by hydrophilic interaction liquid chromatography tandem mass spectrometry |
| topic | infant formula milk powder; human milk oligosaccharides; hydrophilic interaction liquid chromatography-tandem mass spectrometry |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-026.pdf |
| work_keys_str_mv | AT yemengweihuangqianfenglifenghuangyuancaixiaominggaoyuwangtianxipancheng determinationof14humanmilkoligosaccharidesininfantformulamilkpowderbyhydrophilicinteractionliquidchromatographytandemmassspectrometry |