Efficacy of gigantol, a bibenzyl compound, against Porphyromonas gingivalis
Aim This study aims to investigate the inhibitory effect of gigantol against P. gingivalis.Materials and methods The effect of gigantol against the planktonic culture of P. gingivalis was determined by broth microdilution and CFU assay. In addition, bacterial cell surface hydrophobicity and aggregat...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2025.2525234 |
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| author | Arthit Klaophimai Nattanon Kosulwat Thongtos Saeghueng Tanadon Sirijaruworn Bunyawat Ua-Arak Sucharat Sanongkiet Sirinthip Klaophimai Pongsak Utaisincharoen Matsayapan Pudla |
| author_facet | Arthit Klaophimai Nattanon Kosulwat Thongtos Saeghueng Tanadon Sirijaruworn Bunyawat Ua-Arak Sucharat Sanongkiet Sirinthip Klaophimai Pongsak Utaisincharoen Matsayapan Pudla |
| author_sort | Arthit Klaophimai |
| collection | DOAJ |
| description | Aim This study aims to investigate the inhibitory effect of gigantol against P. gingivalis.Materials and methods The effect of gigantol against the planktonic culture of P. gingivalis was determined by broth microdilution and CFU assay. In addition, bacterial cell surface hydrophobicity and aggregation were elucidated. Confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) were performed to observe biofilm thickness and the biofilm structure, respectively. The gingipain-related genes were evaluated using qPCR. Moreover, molecular docking analysis has also detected the interaction between gigantol and gingipains. Finally, the cytotoxicity effect of gigantol on human gingival fibroblasts (HGFs) was also observed by MTT assay.Results The MIC and MBC of gigantol against planktonic P. gingivalis were 0.312 mg/mL. The findings indicated that the effect of gigantol at sub-MIC concentrations can also suppress bacterial growth. Additionally, this compound increased cell surface hydrophobicity and aggregation. CLSM images demonstrated its inhibitory effect on the pre-formed biofilm of P. gingivalis. SEM exhibited that gigantol could affect the bacterial membrane. The downregulation of gingipain-related gene expression was observed. Moreover, molecular docking showed that this compound blocks Kpg and RgpB proteases. Furthermore, the cytotoxicity of gigantol on HGFs exhibited less toxicity than 0.12% CHX.Conclusion Our findings indicated that gigantol inhibits the P. gingivalis biofilm and establishment, which may lead to a potential therapeutic compound for periodontitis patients. |
| format | Article |
| id | doaj-art-27d3eacc592142a786cc0fcf5b543f4a |
| institution | Kabale University |
| issn | 2000-2297 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-27d3eacc592142a786cc0fcf5b543f4a2025-08-20T03:30:20ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972025-12-0117110.1080/20002297.2025.2525234Efficacy of gigantol, a bibenzyl compound, against Porphyromonas gingivalisArthit Klaophimai0Nattanon Kosulwat1Thongtos Saeghueng2Tanadon Sirijaruworn3Bunyawat Ua-Arak4Sucharat Sanongkiet5Sirinthip Klaophimai6Pongsak Utaisincharoen7Matsayapan Pudla8Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, ThailandFaculty of Dentistry, Mahidol University, Bangkok, ThailandFaculty of Dentistry, Mahidol University, Bangkok, ThailandFaculty of Dentistry, Mahidol University, Bangkok, ThailandFaculty of Dentistry, Mahidol University, Bangkok, ThailandDepartment of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom, ThailandDepartment of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok, ThailandDepartment of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, ThailandAim This study aims to investigate the inhibitory effect of gigantol against P. gingivalis.Materials and methods The effect of gigantol against the planktonic culture of P. gingivalis was determined by broth microdilution and CFU assay. In addition, bacterial cell surface hydrophobicity and aggregation were elucidated. Confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) were performed to observe biofilm thickness and the biofilm structure, respectively. The gingipain-related genes were evaluated using qPCR. Moreover, molecular docking analysis has also detected the interaction between gigantol and gingipains. Finally, the cytotoxicity effect of gigantol on human gingival fibroblasts (HGFs) was also observed by MTT assay.Results The MIC and MBC of gigantol against planktonic P. gingivalis were 0.312 mg/mL. The findings indicated that the effect of gigantol at sub-MIC concentrations can also suppress bacterial growth. Additionally, this compound increased cell surface hydrophobicity and aggregation. CLSM images demonstrated its inhibitory effect on the pre-formed biofilm of P. gingivalis. SEM exhibited that gigantol could affect the bacterial membrane. The downregulation of gingipain-related gene expression was observed. Moreover, molecular docking showed that this compound blocks Kpg and RgpB proteases. Furthermore, the cytotoxicity of gigantol on HGFs exhibited less toxicity than 0.12% CHX.Conclusion Our findings indicated that gigantol inhibits the P. gingivalis biofilm and establishment, which may lead to a potential therapeutic compound for periodontitis patients.https://www.tandfonline.com/doi/10.1080/20002297.2025.2525234Biofilmgigantolmolecular dockingperiodontal diseasePorphyromonas gingivalis |
| spellingShingle | Arthit Klaophimai Nattanon Kosulwat Thongtos Saeghueng Tanadon Sirijaruworn Bunyawat Ua-Arak Sucharat Sanongkiet Sirinthip Klaophimai Pongsak Utaisincharoen Matsayapan Pudla Efficacy of gigantol, a bibenzyl compound, against Porphyromonas gingivalis Journal of Oral Microbiology Biofilm gigantol molecular docking periodontal disease Porphyromonas gingivalis |
| title | Efficacy of gigantol, a bibenzyl compound, against Porphyromonas gingivalis |
| title_full | Efficacy of gigantol, a bibenzyl compound, against Porphyromonas gingivalis |
| title_fullStr | Efficacy of gigantol, a bibenzyl compound, against Porphyromonas gingivalis |
| title_full_unstemmed | Efficacy of gigantol, a bibenzyl compound, against Porphyromonas gingivalis |
| title_short | Efficacy of gigantol, a bibenzyl compound, against Porphyromonas gingivalis |
| title_sort | efficacy of gigantol a bibenzyl compound against porphyromonas gingivalis |
| topic | Biofilm gigantol molecular docking periodontal disease Porphyromonas gingivalis |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2025.2525234 |
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