IL-17-Expressing CD4+ and CD8+ T Lymphocytes in Human Toxoplasmosis
This study aimed to measure the synthesis of Th1 and Th2 cytokines by mononuclear cells after culture with live T. gondii and identified Th17 (CD4+) and Tc17 (CD8+) cells in toxoplasma-seronegative and toxoplasma-seropositive parturient and nonpregnant women. Cytometric bead arrays were used to meas...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/573825 |
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| author | Jéssica Líver Alves Silva Karine Rezende-Oliveira Marcos Vinicius da Silva César Gómez-Hernández Bethânea Crema Peghini Neide Maria Silva José Roberto Mineo Virmondes Rodrigues Júnior |
| author_facet | Jéssica Líver Alves Silva Karine Rezende-Oliveira Marcos Vinicius da Silva César Gómez-Hernández Bethânea Crema Peghini Neide Maria Silva José Roberto Mineo Virmondes Rodrigues Júnior |
| author_sort | Jéssica Líver Alves Silva |
| collection | DOAJ |
| description | This study aimed to measure the synthesis of Th1 and Th2 cytokines by mononuclear cells after culture with live T. gondii and identified Th17 (CD4+) and Tc17 (CD8+) cells in toxoplasma-seronegative and toxoplasma-seropositive parturient and nonpregnant women. Cytometric bead arrays were used to measure cytokine levels (IL-2, TNF-α, IFN-γ, IL-4, IL-5, and IL-10); immunophenotyping was used to characterize Th17 and Tc17 cells, and the cells were stained with antibodies against CD4+ and CD8+ T cells expressing IL-17. The addition of tachyzoites to cell cultures induced the synthesis of IL-5, IL-10, and TNF-α by cells from seronegative parturient women and of IL-5 and IL-10 by cells from seropositive, nonpregnant women. We observed a lower level of IL-17-expressing CD4+ and CD8+ T lymphocytes in cultures of cells from seronegative and seropositive parturient and nonpregnant women that were stimulated with tachyzoites, whereas analysis of the CD4+ and CD8+ T cell populations showed a higher level of CD4+ T cells compared with CD8+ T cells. These results suggest that the cytokine pattern and IL-17-expressing CD4+ and CD8+ T lymphocytes may have important roles in the inflammatory response to T. gondii, thus contributing to the maintenance of pregnancy and control of parasite invasion and replication. |
| format | Article |
| id | doaj-art-27bd4eff25dc493c87719988d4c59ce7 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-27bd4eff25dc493c87719988d4c59ce72025-08-20T03:54:41ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/573825573825IL-17-Expressing CD4+ and CD8+ T Lymphocytes in Human ToxoplasmosisJéssica Líver Alves Silva0Karine Rezende-Oliveira1Marcos Vinicius da Silva2César Gómez-Hernández3Bethânea Crema Peghini4Neide Maria Silva5José Roberto Mineo6Virmondes Rodrigues Júnior7Course of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, BrazilLaboratory of Biomedical Sciences, Federal University of Uberlândia, Rua 20, No. 1600, 38304-402 Ituiutaba, MG, BrazilCourse of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, BrazilCourse of Tropical Medicine and Infectology, Laboratory of Parasitology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, BrazilCourse of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, BrazilLaboratory of Histology and Embryology, Federal University of Uberlândia, Avenida Pará, No. 1720, 38400-902 Uberlândia, MG, BrazilLaboratory of Immunology, Federal University of Uberlândia, Avenida Pará, No. 1720, 38400-902 Uberlândia, MG, BrazilCourse of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, BrazilThis study aimed to measure the synthesis of Th1 and Th2 cytokines by mononuclear cells after culture with live T. gondii and identified Th17 (CD4+) and Tc17 (CD8+) cells in toxoplasma-seronegative and toxoplasma-seropositive parturient and nonpregnant women. Cytometric bead arrays were used to measure cytokine levels (IL-2, TNF-α, IFN-γ, IL-4, IL-5, and IL-10); immunophenotyping was used to characterize Th17 and Tc17 cells, and the cells were stained with antibodies against CD4+ and CD8+ T cells expressing IL-17. The addition of tachyzoites to cell cultures induced the synthesis of IL-5, IL-10, and TNF-α by cells from seronegative parturient women and of IL-5 and IL-10 by cells from seropositive, nonpregnant women. We observed a lower level of IL-17-expressing CD4+ and CD8+ T lymphocytes in cultures of cells from seronegative and seropositive parturient and nonpregnant women that were stimulated with tachyzoites, whereas analysis of the CD4+ and CD8+ T cell populations showed a higher level of CD4+ T cells compared with CD8+ T cells. These results suggest that the cytokine pattern and IL-17-expressing CD4+ and CD8+ T lymphocytes may have important roles in the inflammatory response to T. gondii, thus contributing to the maintenance of pregnancy and control of parasite invasion and replication.http://dx.doi.org/10.1155/2014/573825 |
| spellingShingle | Jéssica Líver Alves Silva Karine Rezende-Oliveira Marcos Vinicius da Silva César Gómez-Hernández Bethânea Crema Peghini Neide Maria Silva José Roberto Mineo Virmondes Rodrigues Júnior IL-17-Expressing CD4+ and CD8+ T Lymphocytes in Human Toxoplasmosis Mediators of Inflammation |
| title | IL-17-Expressing CD4+ and CD8+ T Lymphocytes in Human Toxoplasmosis |
| title_full | IL-17-Expressing CD4+ and CD8+ T Lymphocytes in Human Toxoplasmosis |
| title_fullStr | IL-17-Expressing CD4+ and CD8+ T Lymphocytes in Human Toxoplasmosis |
| title_full_unstemmed | IL-17-Expressing CD4+ and CD8+ T Lymphocytes in Human Toxoplasmosis |
| title_short | IL-17-Expressing CD4+ and CD8+ T Lymphocytes in Human Toxoplasmosis |
| title_sort | il 17 expressing cd4 and cd8 t lymphocytes in human toxoplasmosis |
| url | http://dx.doi.org/10.1155/2014/573825 |
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