Transient degradation of NF-κB proteins in macrophages after interaction with mast cell granules
The exposure of the macrophage cell line, J774 to mast cell granules (MCG) led to the form ation of altered nuclear transcription factor proteins (NFκBx), which had faster electrophoretic mobility than the p50 homodimer of NF-κB, but retained comparable DNA binding capacity. Antibodies to N-terminal...
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359890776 |
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| author | Noriko Ito Yuai Li Tsuneo Suzuki Daniel J. Stechschulte Kottarappat N. Dileepan |
| author_facet | Noriko Ito Yuai Li Tsuneo Suzuki Daniel J. Stechschulte Kottarappat N. Dileepan |
| author_sort | Noriko Ito |
| collection | DOAJ |
| description | The exposure of the macrophage cell line, J774 to mast cell granules (MCG) led to the form ation of altered nuclear transcription factor proteins (NFκBx), which had faster electrophoretic mobility than the p50 homodimer of NF-κB, but retained comparable DNA binding capacity. Antibodies to N-terminal peptides of p50, p52, p65 or c-Rel supershifted only a fraction of NF-κBx. Western blot analyses revealed that nuclear p65 and c-Rel were progressively degraded after exposure to MCG, whereas nuclear p50 appeared to be unaffected. In contrast, cytoplasmic p50, p65, c-Rel as well as IkBα remained intact after MCG treatment, although p52 was clearly degraded. In comparison to J774 cells, incubation of m ouse peritoneal macrophages with MCG resulted in more extensive alterations to NF-κB proteins. The alterations in NF-κB proteins did not affect the expression of inducible nitric oxide synthase (iNOS) or TNF-α mRNA in J774 cells. These data indicate that exposure of J774 cells to MCG leads to generation of altered nuclear p52, p65 and c-Rel, which retain intact N-terminal peptides, specific oligonucleotide binding and transactivating activity. On the other hand, in peritoneal macrophages, MCG induce more extensive modifications to NF-κB proteins with associated inhibition of iNOS or TNF-α mRNA expression. |
| format | Article |
| id | doaj-art-27b95133ecf14057976387fe0f1627f5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-27b95133ecf14057976387fe0f1627f52025-02-03T05:53:32ZengWileyMediators of Inflammation0962-93511466-18611998-01-017639740710.1080/09629359890776Transient degradation of NF-κB proteins in macrophages after interaction with mast cell granulesNoriko Ito0Yuai Li1Tsuneo Suzuki2Daniel J. Stechschulte3Kottarappat N. Dileepan4Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USADepartment of Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USADepartment of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USADepartment of Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USADepartment of Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USAThe exposure of the macrophage cell line, J774 to mast cell granules (MCG) led to the form ation of altered nuclear transcription factor proteins (NFκBx), which had faster electrophoretic mobility than the p50 homodimer of NF-κB, but retained comparable DNA binding capacity. Antibodies to N-terminal peptides of p50, p52, p65 or c-Rel supershifted only a fraction of NF-κBx. Western blot analyses revealed that nuclear p65 and c-Rel were progressively degraded after exposure to MCG, whereas nuclear p50 appeared to be unaffected. In contrast, cytoplasmic p50, p65, c-Rel as well as IkBα remained intact after MCG treatment, although p52 was clearly degraded. In comparison to J774 cells, incubation of m ouse peritoneal macrophages with MCG resulted in more extensive alterations to NF-κB proteins. The alterations in NF-κB proteins did not affect the expression of inducible nitric oxide synthase (iNOS) or TNF-α mRNA in J774 cells. These data indicate that exposure of J774 cells to MCG leads to generation of altered nuclear p52, p65 and c-Rel, which retain intact N-terminal peptides, specific oligonucleotide binding and transactivating activity. On the other hand, in peritoneal macrophages, MCG induce more extensive modifications to NF-κB proteins with associated inhibition of iNOS or TNF-α mRNA expression.http://dx.doi.org/10.1080/09629359890776MacrophagesMast cellsMast cell granulesNF-κBNitric oxide synthaseTNF-α. |
| spellingShingle | Noriko Ito Yuai Li Tsuneo Suzuki Daniel J. Stechschulte Kottarappat N. Dileepan Transient degradation of NF-κB proteins in macrophages after interaction with mast cell granules Mediators of Inflammation Macrophages Mast cells Mast cell granules NF-κB Nitric oxide synthase TNF-α. |
| title | Transient degradation of NF-κB proteins in macrophages after interaction
with mast cell granules |
| title_full | Transient degradation of NF-κB proteins in macrophages after interaction
with mast cell granules |
| title_fullStr | Transient degradation of NF-κB proteins in macrophages after interaction
with mast cell granules |
| title_full_unstemmed | Transient degradation of NF-κB proteins in macrophages after interaction
with mast cell granules |
| title_short | Transient degradation of NF-κB proteins in macrophages after interaction
with mast cell granules |
| title_sort | transient degradation of nf κb proteins in macrophages after interaction with mast cell granules |
| topic | Macrophages Mast cells Mast cell granules NF-κB Nitric oxide synthase TNF-α. |
| url | http://dx.doi.org/10.1080/09629359890776 |
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