The microbial communities and metabolic profiles of follicular fluid in patients with premature ovarian insufficiency
IntroductionPremature ovarian insufficiency (POI) is a condition characterized by ovarian dysfunction occurring before the age of 40, and its etiology is multifactorial, including genetic, immunological, infectious, environmental, and iatrogenic factors, with over half of the cases remaining unexpla...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2024.1447397/full |
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| author | Wei Wang Wei Wang Mingming Shu Jianhua Li Qihang Wang Wendan Zhang Ye Wang Yiming Guo Yanbin Cheng Honghong Jiang Chunlan Song Yuan Liu Wei Shang Wei Shang |
| author_facet | Wei Wang Wei Wang Mingming Shu Jianhua Li Qihang Wang Wendan Zhang Ye Wang Yiming Guo Yanbin Cheng Honghong Jiang Chunlan Song Yuan Liu Wei Shang Wei Shang |
| author_sort | Wei Wang |
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| description | IntroductionPremature ovarian insufficiency (POI) is a condition characterized by ovarian dysfunction occurring before the age of 40, and its etiology is multifactorial, including genetic, immunological, infectious, environmental, and iatrogenic factors, with over half of the cases remaining unexplained. Whether the microbial communities and metabolites in follicular fluid, which is the direct microenvironment for oocyte survival, are related to POI has not been reported.MethodsIn this study, Follicular fluid samples of 26 patients with POI and 27 controls with a normal ovarian reserve were collected and analyzed using 16S rDNA sequencing and untargeted metabolomics. Conjoint analysis was performed to identify key microbial communities and metabolites that might be involved in POI.ResultsPatients with POI exhibited significant alterations in microbial richness and diversity and metabolic profile in their follicular fluid. The downregulation of ABC transporters and upregulation of the citrate cycle (TCA cycle) might be critical for the development and progression of POI. G-Rhodopseudomonas and g-Caulobacter were identified as key microbial genera, while L-aspartic acid, citrate, isoleucine, and cytidine were identified as key metabolites.DiscussionThese findings offer novel insights into the pathogenesis of POI and might pave the way for improved clinical outcomes for individuals with POI. |
| format | Article |
| id | doaj-art-27b911d5a1864fffb13c8da85773b7ce |
| institution | Kabale University |
| issn | 1664-2392 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Endocrinology |
| spelling | doaj-art-27b911d5a1864fffb13c8da85773b7ce2025-01-07T05:23:47ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922025-01-011510.3389/fendo.2024.14473971447397The microbial communities and metabolic profiles of follicular fluid in patients with premature ovarian insufficiencyWei Wang0Wei Wang1Mingming Shu2Jianhua Li3Qihang Wang4Wendan Zhang5Ye Wang6Yiming Guo7Yanbin Cheng8Honghong Jiang9Chunlan Song10Yuan Liu11Wei Shang12Wei Shang13Department of Obstetrics and Gynecology, The Seventh Medical Center of Chinese People's Liberation Army (PLA) General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Sixth Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese People's Liberation Army (PLA) General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese People's Liberation Army (PLA) General Hospital, Beijing, ChinaFaculty of Pediatrics, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese People's Liberation Army (PLA) General Hospital, Beijing, ChinaDepartment of Biology, Kenneth P. Dietrich School of Art and Science, University of Pittsburgh, Pittsburgh, PA, United StatesDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese People's Liberation Army (PLA) General Hospital, Beijing, ChinaFaculty of Pediatrics, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese People's Liberation Army (PLA) General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese People's Liberation Army (PLA) General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, The Seventh Medical Center of Chinese People's Liberation Army (PLA) General Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, Chinese PLA General Hospital, Beijing, ChinaIntroductionPremature ovarian insufficiency (POI) is a condition characterized by ovarian dysfunction occurring before the age of 40, and its etiology is multifactorial, including genetic, immunological, infectious, environmental, and iatrogenic factors, with over half of the cases remaining unexplained. Whether the microbial communities and metabolites in follicular fluid, which is the direct microenvironment for oocyte survival, are related to POI has not been reported.MethodsIn this study, Follicular fluid samples of 26 patients with POI and 27 controls with a normal ovarian reserve were collected and analyzed using 16S rDNA sequencing and untargeted metabolomics. Conjoint analysis was performed to identify key microbial communities and metabolites that might be involved in POI.ResultsPatients with POI exhibited significant alterations in microbial richness and diversity and metabolic profile in their follicular fluid. The downregulation of ABC transporters and upregulation of the citrate cycle (TCA cycle) might be critical for the development and progression of POI. G-Rhodopseudomonas and g-Caulobacter were identified as key microbial genera, while L-aspartic acid, citrate, isoleucine, and cytidine were identified as key metabolites.DiscussionThese findings offer novel insights into the pathogenesis of POI and might pave the way for improved clinical outcomes for individuals with POI.https://www.frontiersin.org/articles/10.3389/fendo.2024.1447397/fullpremature ovarian insufficiency16S rDNA amplicon sequencingmetabolomicsABC transporterscitrate cycle (TCA cycle) |
| spellingShingle | Wei Wang Wei Wang Mingming Shu Jianhua Li Qihang Wang Wendan Zhang Ye Wang Yiming Guo Yanbin Cheng Honghong Jiang Chunlan Song Yuan Liu Wei Shang Wei Shang The microbial communities and metabolic profiles of follicular fluid in patients with premature ovarian insufficiency Frontiers in Endocrinology premature ovarian insufficiency 16S rDNA amplicon sequencing metabolomics ABC transporters citrate cycle (TCA cycle) |
| title | The microbial communities and metabolic profiles of follicular fluid in patients with premature ovarian insufficiency |
| title_full | The microbial communities and metabolic profiles of follicular fluid in patients with premature ovarian insufficiency |
| title_fullStr | The microbial communities and metabolic profiles of follicular fluid in patients with premature ovarian insufficiency |
| title_full_unstemmed | The microbial communities and metabolic profiles of follicular fluid in patients with premature ovarian insufficiency |
| title_short | The microbial communities and metabolic profiles of follicular fluid in patients with premature ovarian insufficiency |
| title_sort | microbial communities and metabolic profiles of follicular fluid in patients with premature ovarian insufficiency |
| topic | premature ovarian insufficiency 16S rDNA amplicon sequencing metabolomics ABC transporters citrate cycle (TCA cycle) |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2024.1447397/full |
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