Plasmid role in agar utilization by Pseudomonas sp. HK1
Forty different samples (water and soil) were collected from different places in Iraq and Syria. Only (6) isolates showed the ability to grow and utilize agar as a sole source of carbon and energy. Morphological, cultural characterization and biochemical tests confirmed that These isolates belongi...
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| Format: | Article |
| Language: | English |
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University of Baghdad, College of Science for Women
2007-03-01
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| Series: | مجلة بغداد للعلوم |
| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/766 |
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| author | Baghdad Science Journal |
| author_facet | Baghdad Science Journal |
| author_sort | Baghdad Science Journal |
| collection | DOAJ |
| description | Forty different samples (water and soil) were collected from different places in Iraq and Syria. Only (6) isolates showed the ability to grow and utilize agar as a sole source of carbon and energy. Morphological, cultural characterization and biochemical tests confirmed that These isolates belonging to genus Pseudomonas (HK1-HK6) .Plasmid profiles results showed that these isolates were harbored (2 -3) small Plasmids . HK1 isolate was selected because of its efficiency and ability to grow in high density on agar media for transformation and curing experiments, these were checked by transformation experiments after their expression in E. coli MM294. The genes responsible for agar utilization were located on these plasmids . These results were confirmed by data obtained from curing experiments. |
| format | Article |
| id | doaj-art-27ae196929ca49a79eeae4fa862375e8 |
| institution | DOAJ |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2007-03-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-27ae196929ca49a79eeae4fa862375e82025-08-20T02:54:57ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862007-03-014110.21123/bsj.4.1.41-46Plasmid role in agar utilization by Pseudomonas sp. HK1Baghdad Science JournalForty different samples (water and soil) were collected from different places in Iraq and Syria. Only (6) isolates showed the ability to grow and utilize agar as a sole source of carbon and energy. Morphological, cultural characterization and biochemical tests confirmed that These isolates belonging to genus Pseudomonas (HK1-HK6) .Plasmid profiles results showed that these isolates were harbored (2 -3) small Plasmids . HK1 isolate was selected because of its efficiency and ability to grow in high density on agar media for transformation and curing experiments, these were checked by transformation experiments after their expression in E. coli MM294. The genes responsible for agar utilization were located on these plasmids . These results were confirmed by data obtained from curing experiments.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/766 |
| spellingShingle | Baghdad Science Journal Plasmid role in agar utilization by Pseudomonas sp. HK1 مجلة بغداد للعلوم |
| title | Plasmid role in agar utilization by Pseudomonas sp. HK1 |
| title_full | Plasmid role in agar utilization by Pseudomonas sp. HK1 |
| title_fullStr | Plasmid role in agar utilization by Pseudomonas sp. HK1 |
| title_full_unstemmed | Plasmid role in agar utilization by Pseudomonas sp. HK1 |
| title_short | Plasmid role in agar utilization by Pseudomonas sp. HK1 |
| title_sort | plasmid role in agar utilization by pseudomonas sp hk1 |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/766 |
| work_keys_str_mv | AT baghdadsciencejournal plasmidroleinagarutilizationbypseudomonassphk1 |