The antimicrobial efficacy of rutin encapsulated chitosan versus multidrug-resistant Pseudomonas aeruginosa
Abstract Pseudomonas aeruginosa (P. aeruginosa) is a predominant etiological agent of nosocomial infections. This research examined the efficacy of rutin encapsulated in chitosan nanoparticles (Rut-CS) in inhibiting the proliferation of multidrug-resistant P. aeruginosa and diminishing biofilm forma...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-06873-2 |
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| author | Helia Ramezani Hossein Sazegar Leila Rouhi |
| author_facet | Helia Ramezani Hossein Sazegar Leila Rouhi |
| author_sort | Helia Ramezani |
| collection | DOAJ |
| description | Abstract Pseudomonas aeruginosa (P. aeruginosa) is a predominant etiological agent of nosocomial infections. This research examined the efficacy of rutin encapsulated in chitosan nanoparticles (Rut-CS) in inhibiting the proliferation of multidrug-resistant P. aeruginosa and diminishing biofilm formation. SEM, DLS and FTIR analyzed the nanoparticles to ascertain their shape. The antibacterial activity was evaluated using agar well diffusion. The findings indicated that Rut-CS nanoparticles had considerable antibacterial efficacy and substantially reduced the expression of critical biofilm-associated genes, lasR and AlgD. The Rut-CS nanoparticles exhibited no toxicity to human dermal fibroblast (HDF) cells at the minimal inhibitory dose. The data indicate that Rut-CS nanoparticles may serve as a viable technique to address highly resistant P. aeruginosa infections, facilitating novel antibacterial research methodologies. |
| format | Article |
| id | doaj-art-27ab6ebbe4b44c4e87b34015da6e0a02 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-27ab6ebbe4b44c4e87b34015da6e0a022025-08-20T03:45:23ZengNature PortfolioScientific Reports2045-23222025-07-0115111310.1038/s41598-025-06873-2The antimicrobial efficacy of rutin encapsulated chitosan versus multidrug-resistant Pseudomonas aeruginosaHelia Ramezani0Hossein Sazegar1Leila Rouhi2Department of Biology, ShK.C., Islamic Azad UniversityDepartment of Biology, ShK.C., Islamic Azad UniversityDepartment of Biology, ShK.C., Islamic Azad UniversityAbstract Pseudomonas aeruginosa (P. aeruginosa) is a predominant etiological agent of nosocomial infections. This research examined the efficacy of rutin encapsulated in chitosan nanoparticles (Rut-CS) in inhibiting the proliferation of multidrug-resistant P. aeruginosa and diminishing biofilm formation. SEM, DLS and FTIR analyzed the nanoparticles to ascertain their shape. The antibacterial activity was evaluated using agar well diffusion. The findings indicated that Rut-CS nanoparticles had considerable antibacterial efficacy and substantially reduced the expression of critical biofilm-associated genes, lasR and AlgD. The Rut-CS nanoparticles exhibited no toxicity to human dermal fibroblast (HDF) cells at the minimal inhibitory dose. The data indicate that Rut-CS nanoparticles may serve as a viable technique to address highly resistant P. aeruginosa infections, facilitating novel antibacterial research methodologies.https://doi.org/10.1038/s41598-025-06873-2P. aeruginosaMulti drug resistantRutinChitosan |
| spellingShingle | Helia Ramezani Hossein Sazegar Leila Rouhi The antimicrobial efficacy of rutin encapsulated chitosan versus multidrug-resistant Pseudomonas aeruginosa Scientific Reports P. aeruginosa Multi drug resistant Rutin Chitosan |
| title | The antimicrobial efficacy of rutin encapsulated chitosan versus multidrug-resistant Pseudomonas aeruginosa |
| title_full | The antimicrobial efficacy of rutin encapsulated chitosan versus multidrug-resistant Pseudomonas aeruginosa |
| title_fullStr | The antimicrobial efficacy of rutin encapsulated chitosan versus multidrug-resistant Pseudomonas aeruginosa |
| title_full_unstemmed | The antimicrobial efficacy of rutin encapsulated chitosan versus multidrug-resistant Pseudomonas aeruginosa |
| title_short | The antimicrobial efficacy of rutin encapsulated chitosan versus multidrug-resistant Pseudomonas aeruginosa |
| title_sort | antimicrobial efficacy of rutin encapsulated chitosan versus multidrug resistant pseudomonas aeruginosa |
| topic | P. aeruginosa Multi drug resistant Rutin Chitosan |
| url | https://doi.org/10.1038/s41598-025-06873-2 |
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