The antimicrobial efficacy of rutin encapsulated chitosan versus multidrug-resistant Pseudomonas aeruginosa

Abstract Pseudomonas aeruginosa (P. aeruginosa) is a predominant etiological agent of nosocomial infections. This research examined the efficacy of rutin encapsulated in chitosan nanoparticles (Rut-CS) in inhibiting the proliferation of multidrug-resistant P. aeruginosa and diminishing biofilm forma...

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Bibliographic Details
Main Authors: Helia Ramezani, Hossein Sazegar, Leila Rouhi
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-06873-2
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Summary:Abstract Pseudomonas aeruginosa (P. aeruginosa) is a predominant etiological agent of nosocomial infections. This research examined the efficacy of rutin encapsulated in chitosan nanoparticles (Rut-CS) in inhibiting the proliferation of multidrug-resistant P. aeruginosa and diminishing biofilm formation. SEM, DLS and FTIR analyzed the nanoparticles to ascertain their shape. The antibacterial activity was evaluated using agar well diffusion. The findings indicated that Rut-CS nanoparticles had considerable antibacterial efficacy and substantially reduced the expression of critical biofilm-associated genes, lasR and AlgD. The Rut-CS nanoparticles exhibited no toxicity to human dermal fibroblast (HDF) cells at the minimal inhibitory dose. The data indicate that Rut-CS nanoparticles may serve as a viable technique to address highly resistant P. aeruginosa infections, facilitating novel antibacterial research methodologies.
ISSN:2045-2322