New Data on <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> in Thailand by DNA Barcoding and Morphological Methods
A combination of morphological analysis and DNA barcoding (partial sequences of the cytochrome c oxidase I (COI) gene) was used to differentiate four gobiid fish species in the family Oxudercidae. <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> were found...
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MDPI AG
2025-03-01
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| author | Siriluck Tuncharoen Paiboon Panase Nontree Panprommin Eakapol Wangkahart Supranee Ruenkoed Keatipong Mongkolwit Dutrudi Panprommin |
| author_facet | Siriluck Tuncharoen Paiboon Panase Nontree Panprommin Eakapol Wangkahart Supranee Ruenkoed Keatipong Mongkolwit Dutrudi Panprommin |
| author_sort | Siriluck Tuncharoen |
| collection | DOAJ |
| description | A combination of morphological analysis and DNA barcoding (partial sequences of the cytochrome c oxidase I (COI) gene) was used to differentiate four gobiid fish species in the family Oxudercidae. <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> were found in Thailand, while <i>Eugnathogobius siamensis</i> and <i>Pseudogobiopsis oligactis</i> were used for comparative purposes. Morphological identification relied on appearances, counts, and measurements. The 707-base pair COI sequences from eleven samples of four gobiid species were compared with reference sequences in public databases to confirm their scientific names. The average AT content was 51.8 ± 0.5% and the GC content was 48.2 ± 0.5%. Intraspecific genetic distances ranged from 0.00–0.28%, while interspecific genetic distances ranged from 0.86–16.63%. A neighbor-joining (NJ) phylogenetic tree depicted the relationships among the COI sequences of these species. Morphological analysis and COI sequences successfully distinguished the four gobiid species. Notably, the COI sequences of <i>R. chiengmaiensis</i>, <i>R. mekongianus</i>, and <i>E. siamensis</i> were previously unreported, hence, this study is the first report to add their sequences to public databases. These results can serve as valuable information for the management of aquatic resources, conservation, and aquaculture efforts. |
| format | Article |
| id | doaj-art-27a13be80dec4e07bda5d6aab419bf22 |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-27a13be80dec4e07bda5d6aab419bf222025-08-20T02:42:41ZengMDPI AGAnimals2076-26152025-03-0115687110.3390/ani15060871New Data on <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> in Thailand by DNA Barcoding and Morphological MethodsSiriluck Tuncharoen0Paiboon Panase1Nontree Panprommin2Eakapol Wangkahart3Supranee Ruenkoed4Keatipong Mongkolwit5Dutrudi Panprommin6School of Agriculture and Natural Resources, University of Phayao, Phayao 56000, ThailandSchool of Agriculture and Natural Resources, University of Phayao, Phayao 56000, ThailandDepartment of Fisheries, Aquatic Plants and Ornamental Fish Research Institute, Bangkok 10900, ThailandFaculty of Technology, Mahasarakham University, Mahasarakham 44150, ThailandAdvance Pharma Vietnam Co., Ltd., Ho Chi Minh City 10000, VietnamAdvance Pharma Vietnam Co., Ltd., Ho Chi Minh City 10000, VietnamSchool of Agriculture and Natural Resources, University of Phayao, Phayao 56000, ThailandA combination of morphological analysis and DNA barcoding (partial sequences of the cytochrome c oxidase I (COI) gene) was used to differentiate four gobiid fish species in the family Oxudercidae. <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> were found in Thailand, while <i>Eugnathogobius siamensis</i> and <i>Pseudogobiopsis oligactis</i> were used for comparative purposes. Morphological identification relied on appearances, counts, and measurements. The 707-base pair COI sequences from eleven samples of four gobiid species were compared with reference sequences in public databases to confirm their scientific names. The average AT content was 51.8 ± 0.5% and the GC content was 48.2 ± 0.5%. Intraspecific genetic distances ranged from 0.00–0.28%, while interspecific genetic distances ranged from 0.86–16.63%. A neighbor-joining (NJ) phylogenetic tree depicted the relationships among the COI sequences of these species. Morphological analysis and COI sequences successfully distinguished the four gobiid species. Notably, the COI sequences of <i>R. chiengmaiensis</i>, <i>R. mekongianus</i>, and <i>E. siamensis</i> were previously unreported, hence, this study is the first report to add their sequences to public databases. These results can serve as valuable information for the management of aquatic resources, conservation, and aquaculture efforts.https://www.mdpi.com/2076-2615/15/6/871morphological identificationDNA barcodingcytochrome c oxidase Ispecies identification<i>Rhinogobius</i>Oxudercidae |
| spellingShingle | Siriluck Tuncharoen Paiboon Panase Nontree Panprommin Eakapol Wangkahart Supranee Ruenkoed Keatipong Mongkolwit Dutrudi Panprommin New Data on <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> in Thailand by DNA Barcoding and Morphological Methods Animals morphological identification DNA barcoding cytochrome c oxidase I species identification <i>Rhinogobius</i> Oxudercidae |
| title | New Data on <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> in Thailand by DNA Barcoding and Morphological Methods |
| title_full | New Data on <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> in Thailand by DNA Barcoding and Morphological Methods |
| title_fullStr | New Data on <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> in Thailand by DNA Barcoding and Morphological Methods |
| title_full_unstemmed | New Data on <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> in Thailand by DNA Barcoding and Morphological Methods |
| title_short | New Data on <i>Rhinogobius chiengmaiensis</i> and <i>Rhinogobius mekongianus</i> in Thailand by DNA Barcoding and Morphological Methods |
| title_sort | new data on i rhinogobius chiengmaiensis i and i rhinogobius mekongianus i in thailand by dna barcoding and morphological methods |
| topic | morphological identification DNA barcoding cytochrome c oxidase I species identification <i>Rhinogobius</i> Oxudercidae |
| url | https://www.mdpi.com/2076-2615/15/6/871 |
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