Gas Chromatographic/Mass Spectrometric Analysis of Volatile Metabolites in Bovine Vaginal Fluid and Assessment of Their Bioactivity
The chemical profiles of vaginal fluid collected from cows in oestrus and nonoestrus were analysed by gas chromatography-mass spectrometry (GC-MS) to establish any qualitative differences that might have p...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/256106 |
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| Summary: | The chemical
profiles of vaginal fluid collected from cows in
oestrus and nonoestrus were analysed by gas
chromatography-mass spectrometry (GC-MS)
to establish any qualitative differences that
might have potential value in bovine
biocommunication. Eight different organic
compounds were detected using the two
chromatograms. The chemical profiles of oestrus
vaginal fluid were distinguished significantly by
the presence of three specific substances, namely,
trimethylamine, acetic acid, and propionic acid
that were not present in nonoestrus phase. The
oestrus specific synthetic compounds were rubbed
onto the genital region of nonoestrus animals
(dummy cows), and the bulls were allowed to
sniff the genital region and observed sexual
behaviours. The statistical significance was
higher (P<0.001) in bulls exhibiting repeated flehmen and mounting
behaviours towards the mixture of acetic acid, propionic acid, and
trimethylamine as compared to test these compounds separately. It was concluded that the volatile substances present in the bovine vaginal fluid during oestrus may act as chemical communicators. |
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| ISSN: | 1687-8760 1687-8779 |