Effect of a Traditional Chinese Herbal Medicine Formulation on Cell Survival and Apoptosis of MPP+-Treated MES 23.5 Dopaminergic Cells

Progressive degeneration of dopaminergic neurons in the substantia nigra (SN) is implicated in Parkinson’s disease (PD). The efficacy of these currently used drugs is limited while traditional Chinese medicine (TCM) has been used in the management of neurodegenerative diseases for many years. This s...

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Main Authors: Shuifen Ye, Ho Kee Koon, Wen Fan, Yihui Xu, Wei Wei, Chuanshan Xu, Jing Cai
Format: Article
Language:English
Published: Wiley 2017-01-01
Series:Parkinson's Disease
Online Access:http://dx.doi.org/10.1155/2017/4764212
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author Shuifen Ye
Ho Kee Koon
Wen Fan
Yihui Xu
Wei Wei
Chuanshan Xu
Jing Cai
author_facet Shuifen Ye
Ho Kee Koon
Wen Fan
Yihui Xu
Wei Wei
Chuanshan Xu
Jing Cai
author_sort Shuifen Ye
collection DOAJ
description Progressive degeneration of dopaminergic neurons in the substantia nigra (SN) is implicated in Parkinson’s disease (PD). The efficacy of these currently used drugs is limited while traditional Chinese medicine (TCM) has been used in the management of neurodegenerative diseases for many years. This study was designed to evaluate the effect of a modified traditional Chinese herbal medicine decoction, Cong Rong Jing (CRJ), on cell survival and apoptosis of 1-methyl-4-phenylpyridinium- (MPP+-) treated MES23.5 dopaminergic cells. CRJ was prepared as a decoction from three Chinese herbs, namely, Herba Cistanches, Herba Epimedii, and Rhizoma Polygonati. We reported here that CRJ significantly enhanced the cell survival of MES23.5 cells after the exposure of MPP+ and inhibited the production of intracellular reactive oxygen species (ROS) induced by MPP+. CRJ also prevented the MPP+-treated MES23.5 cells from apoptosis by reducing the externalization of phosphatidylserine and enhancing the Bcl-2/Bax protein expression ratio. Signaling proteins such as JAK2, STAT3, and ERK1/2 were also involved in the action of CRJ. Taken together, these results provide a preliminary mechanism to support clinical application of the TCM formulation in PD and possibly other neurodegenerative diseases associated with ROS injury and apoptosis.
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spelling doaj-art-277fc4b3cf9c4caa98fa90832356f3922025-02-03T05:58:14ZengWileyParkinson's Disease2090-80832042-00802017-01-01201710.1155/2017/47642124764212Effect of a Traditional Chinese Herbal Medicine Formulation on Cell Survival and Apoptosis of MPP+-Treated MES 23.5 Dopaminergic CellsShuifen Ye0Ho Kee Koon1Wen Fan2Yihui Xu3Wei Wei4Chuanshan Xu5Jing Cai6Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, ChinaSchool of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong KongDepartment of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, ChinaDepartment of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, ChinaDepartment of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, ChinaSchool of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong KongDepartment of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, ChinaProgressive degeneration of dopaminergic neurons in the substantia nigra (SN) is implicated in Parkinson’s disease (PD). The efficacy of these currently used drugs is limited while traditional Chinese medicine (TCM) has been used in the management of neurodegenerative diseases for many years. This study was designed to evaluate the effect of a modified traditional Chinese herbal medicine decoction, Cong Rong Jing (CRJ), on cell survival and apoptosis of 1-methyl-4-phenylpyridinium- (MPP+-) treated MES23.5 dopaminergic cells. CRJ was prepared as a decoction from three Chinese herbs, namely, Herba Cistanches, Herba Epimedii, and Rhizoma Polygonati. We reported here that CRJ significantly enhanced the cell survival of MES23.5 cells after the exposure of MPP+ and inhibited the production of intracellular reactive oxygen species (ROS) induced by MPP+. CRJ also prevented the MPP+-treated MES23.5 cells from apoptosis by reducing the externalization of phosphatidylserine and enhancing the Bcl-2/Bax protein expression ratio. Signaling proteins such as JAK2, STAT3, and ERK1/2 were also involved in the action of CRJ. Taken together, these results provide a preliminary mechanism to support clinical application of the TCM formulation in PD and possibly other neurodegenerative diseases associated with ROS injury and apoptosis.http://dx.doi.org/10.1155/2017/4764212
spellingShingle Shuifen Ye
Ho Kee Koon
Wen Fan
Yihui Xu
Wei Wei
Chuanshan Xu
Jing Cai
Effect of a Traditional Chinese Herbal Medicine Formulation on Cell Survival and Apoptosis of MPP+-Treated MES 23.5 Dopaminergic Cells
Parkinson's Disease
title Effect of a Traditional Chinese Herbal Medicine Formulation on Cell Survival and Apoptosis of MPP+-Treated MES 23.5 Dopaminergic Cells
title_full Effect of a Traditional Chinese Herbal Medicine Formulation on Cell Survival and Apoptosis of MPP+-Treated MES 23.5 Dopaminergic Cells
title_fullStr Effect of a Traditional Chinese Herbal Medicine Formulation on Cell Survival and Apoptosis of MPP+-Treated MES 23.5 Dopaminergic Cells
title_full_unstemmed Effect of a Traditional Chinese Herbal Medicine Formulation on Cell Survival and Apoptosis of MPP+-Treated MES 23.5 Dopaminergic Cells
title_short Effect of a Traditional Chinese Herbal Medicine Formulation on Cell Survival and Apoptosis of MPP+-Treated MES 23.5 Dopaminergic Cells
title_sort effect of a traditional chinese herbal medicine formulation on cell survival and apoptosis of mpp treated mes 23 5 dopaminergic cells
url http://dx.doi.org/10.1155/2017/4764212
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