Robust phase-retrieval-based X-ray tomography for morphological assessment of early hepatic echinococcosis infection in rats.
Propagation-based phase-contrast computed micro-tomography (PPCT) dominates the non-destructive, three-dimensional inner-structure measurement in synchrotron-based biomedical research due to its simple experimental setup. To quantitatively visualize tiny density variations in soft tissues and organs...
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| Format: | Article |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183396&type=printable |
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| author | Huiqiang Liu Chuanshan Zhang Xiaoxi Fan Yingni Duan Tiqiao Xiao Guohao Du Yanan Fu Haigang Liu Hao Wen |
| author_facet | Huiqiang Liu Chuanshan Zhang Xiaoxi Fan Yingni Duan Tiqiao Xiao Guohao Du Yanan Fu Haigang Liu Hao Wen |
| author_sort | Huiqiang Liu |
| collection | DOAJ |
| description | Propagation-based phase-contrast computed micro-tomography (PPCT) dominates the non-destructive, three-dimensional inner-structure measurement in synchrotron-based biomedical research due to its simple experimental setup. To quantitatively visualize tiny density variations in soft tissues and organs closely related to early pathological morphology, an experimental study of synchrotron-based X-ray PPCT combined with generalized phase and attenuation duality (PAD) phase retrieval was implemented with the hepatic echinococcosis (HE) infection rat model at different stages. We quantitatively analyzed and evaluated the different pathological characterizations of hepatic echinococcosis during the development of this disease via our PAD-based PPCT and especially provided evidence that hepatic alveolar echinococcosis invades the liver tissue and spreads through blood flow systems with abundant blood supply in the early stage. Additionally, the infiltration of tiny vesicles in HE lesions can be clearly observed by our PAD-PPCT technique due to the striking contrast-to-noise ratio (CNR) and mass density resolution, which cannot be found by the medical imaging techniques, such as magnetic resonance imaging (MRI), computed tomography (CT), and ultrasound, in hospitals. The results demonstrated that our PAD-PPCT technique has a great potential for indicating the subtle structural information of pathological changes in soft biomedical specimens, especially helpful for the research of early micro-morphology of diseases. |
| format | Article |
| id | doaj-art-277ebc2629da464ba8d540f217da5bf3 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-277ebc2629da464ba8d540f217da5bf32025-08-20T02:45:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018339610.1371/journal.pone.0183396Robust phase-retrieval-based X-ray tomography for morphological assessment of early hepatic echinococcosis infection in rats.Huiqiang LiuChuanshan ZhangXiaoxi FanYingni DuanTiqiao XiaoGuohao DuYanan FuHaigang LiuHao WenPropagation-based phase-contrast computed micro-tomography (PPCT) dominates the non-destructive, three-dimensional inner-structure measurement in synchrotron-based biomedical research due to its simple experimental setup. To quantitatively visualize tiny density variations in soft tissues and organs closely related to early pathological morphology, an experimental study of synchrotron-based X-ray PPCT combined with generalized phase and attenuation duality (PAD) phase retrieval was implemented with the hepatic echinococcosis (HE) infection rat model at different stages. We quantitatively analyzed and evaluated the different pathological characterizations of hepatic echinococcosis during the development of this disease via our PAD-based PPCT and especially provided evidence that hepatic alveolar echinococcosis invades the liver tissue and spreads through blood flow systems with abundant blood supply in the early stage. Additionally, the infiltration of tiny vesicles in HE lesions can be clearly observed by our PAD-PPCT technique due to the striking contrast-to-noise ratio (CNR) and mass density resolution, which cannot be found by the medical imaging techniques, such as magnetic resonance imaging (MRI), computed tomography (CT), and ultrasound, in hospitals. The results demonstrated that our PAD-PPCT technique has a great potential for indicating the subtle structural information of pathological changes in soft biomedical specimens, especially helpful for the research of early micro-morphology of diseases.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183396&type=printable |
| spellingShingle | Huiqiang Liu Chuanshan Zhang Xiaoxi Fan Yingni Duan Tiqiao Xiao Guohao Du Yanan Fu Haigang Liu Hao Wen Robust phase-retrieval-based X-ray tomography for morphological assessment of early hepatic echinococcosis infection in rats. PLoS ONE |
| title | Robust phase-retrieval-based X-ray tomography for morphological assessment of early hepatic echinococcosis infection in rats. |
| title_full | Robust phase-retrieval-based X-ray tomography for morphological assessment of early hepatic echinococcosis infection in rats. |
| title_fullStr | Robust phase-retrieval-based X-ray tomography for morphological assessment of early hepatic echinococcosis infection in rats. |
| title_full_unstemmed | Robust phase-retrieval-based X-ray tomography for morphological assessment of early hepatic echinococcosis infection in rats. |
| title_short | Robust phase-retrieval-based X-ray tomography for morphological assessment of early hepatic echinococcosis infection in rats. |
| title_sort | robust phase retrieval based x ray tomography for morphological assessment of early hepatic echinococcosis infection in rats |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183396&type=printable |
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