Peroxisomal core structures segregate diverse metabolic pathways
Abstract Peroxisomes are single membrane-bounded oxidative organelles with various metabolic functions including β-oxidation of fatty acids. Peroxisomes of many species confine certain metabolic enzymes into sub-compartments sometimes visible as electron dense cores. Why these structures form is lar...
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-57053-9 |
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| author | Nils Bäcker Julia Ast Domenica Martorana Christian Renicke Jil Berger Cristopher-Nils Mais Marvin Christ Thorsten Stehlik Thomas Heimerl Valentin Wernet Christof Taxis Jan Pané-Farré Michael Bölker Judith M. Klatt Björn Sandrock Kay Oliver Schink Gert Bange Johannes Freitag |
| author_facet | Nils Bäcker Julia Ast Domenica Martorana Christian Renicke Jil Berger Cristopher-Nils Mais Marvin Christ Thorsten Stehlik Thomas Heimerl Valentin Wernet Christof Taxis Jan Pané-Farré Michael Bölker Judith M. Klatt Björn Sandrock Kay Oliver Schink Gert Bange Johannes Freitag |
| author_sort | Nils Bäcker |
| collection | DOAJ |
| description | Abstract Peroxisomes are single membrane-bounded oxidative organelles with various metabolic functions including β-oxidation of fatty acids. Peroxisomes of many species confine certain metabolic enzymes into sub-compartments sometimes visible as electron dense cores. Why these structures form is largely unknown. Here, we report that in the smut fungus Ustilago maydis detergent resistant core structures are enriched for different enzymes excluding several key enzymes of the β-oxidation pathway. This confinement contributes to generation of peroxisome subpopulations that differ in their enzyme content. We identify short amino acid motifs necessary and sufficient for protein self-assembly into aggregates in vitro. The motifs trigger enrichment in cores in vivo and are active in mammalian cells. Perturbation of core assembly via variation of such motifs affects peroxisome function in U. maydis strains challenged with fatty acids. Thus, protein core structures serve to compartmentalize the lumen of peroxisomes thereby preventing interference of biochemical reactions. Metabolic compartmentalization of peroxisomes via assembly of specific proteins may occur in other organisms as well. |
| format | Article |
| id | doaj-art-277df0c4a8d04c409a070aa8bb8fb9fb |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-277df0c4a8d04c409a070aa8bb8fb9fb2025-08-20T02:15:00ZengNature PortfolioNature Communications2041-17232025-02-0116111610.1038/s41467-025-57053-9Peroxisomal core structures segregate diverse metabolic pathwaysNils Bäcker0Julia Ast1Domenica Martorana2Christian Renicke3Jil Berger4Cristopher-Nils Mais5Marvin Christ6Thorsten Stehlik7Thomas Heimerl8Valentin Wernet9Christof Taxis10Jan Pané-Farré11Michael Bölker12Judith M. Klatt13Björn Sandrock14Kay Oliver Schink15Gert Bange16Johannes Freitag17Center for Synthetic Microbiology (SYNMIKRO), Philipps-University MarburgDepartment of Biology, Philipps-University MarburgDepartment of Biology, Philipps-University MarburgDepartment of Biology, Philipps-University MarburgCenter for Synthetic Microbiology (SYNMIKRO), Philipps-University MarburgCenter for Synthetic Microbiology (SYNMIKRO), Philipps-University MarburgCenter for Synthetic Microbiology (SYNMIKRO), Philipps-University MarburgDepartment of Biology, Philipps-University MarburgCenter for Synthetic Microbiology (SYNMIKRO), Philipps-University MarburgDepartment of Microbiology, Institute for Applied Biosciences, KITCenter for Synthetic Microbiology (SYNMIKRO), Philipps-University MarburgCenter for Synthetic Microbiology (SYNMIKRO), Philipps-University MarburgCenter for Synthetic Microbiology (SYNMIKRO), Philipps-University MarburgCenter for Synthetic Microbiology (SYNMIKRO), Philipps-University MarburgDepartment of Biology, Philipps-University MarburgCentre for Cancer Cell Reprogramming, Faculty of Medicine, University of Oslo, MontebelloCenter for Synthetic Microbiology (SYNMIKRO), Philipps-University MarburgCenter for Synthetic Microbiology (SYNMIKRO), Philipps-University MarburgAbstract Peroxisomes are single membrane-bounded oxidative organelles with various metabolic functions including β-oxidation of fatty acids. Peroxisomes of many species confine certain metabolic enzymes into sub-compartments sometimes visible as electron dense cores. Why these structures form is largely unknown. Here, we report that in the smut fungus Ustilago maydis detergent resistant core structures are enriched for different enzymes excluding several key enzymes of the β-oxidation pathway. This confinement contributes to generation of peroxisome subpopulations that differ in their enzyme content. We identify short amino acid motifs necessary and sufficient for protein self-assembly into aggregates in vitro. The motifs trigger enrichment in cores in vivo and are active in mammalian cells. Perturbation of core assembly via variation of such motifs affects peroxisome function in U. maydis strains challenged with fatty acids. Thus, protein core structures serve to compartmentalize the lumen of peroxisomes thereby preventing interference of biochemical reactions. Metabolic compartmentalization of peroxisomes via assembly of specific proteins may occur in other organisms as well.https://doi.org/10.1038/s41467-025-57053-9 |
| spellingShingle | Nils Bäcker Julia Ast Domenica Martorana Christian Renicke Jil Berger Cristopher-Nils Mais Marvin Christ Thorsten Stehlik Thomas Heimerl Valentin Wernet Christof Taxis Jan Pané-Farré Michael Bölker Judith M. Klatt Björn Sandrock Kay Oliver Schink Gert Bange Johannes Freitag Peroxisomal core structures segregate diverse metabolic pathways Nature Communications |
| title | Peroxisomal core structures segregate diverse metabolic pathways |
| title_full | Peroxisomal core structures segregate diverse metabolic pathways |
| title_fullStr | Peroxisomal core structures segregate diverse metabolic pathways |
| title_full_unstemmed | Peroxisomal core structures segregate diverse metabolic pathways |
| title_short | Peroxisomal core structures segregate diverse metabolic pathways |
| title_sort | peroxisomal core structures segregate diverse metabolic pathways |
| url | https://doi.org/10.1038/s41467-025-57053-9 |
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