Efficacy and safety of TiF4 varnish in preventing erosive tooth wear in a rat animal model
Abstract An animal model was applied to develop erosive tooth wear (ETW) and to evaluate the efficacy and safety of titanium tetrafluoride (TiF4) in preventing ETW. Forty-eight male Wistar rats were divided into three groups (n = 16): TiF4 (2.45% F−), NaF (2.45% F−) and placebo varnishes. Eight from...
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Nature Portfolio
2024-11-01
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| Online Access: | https://doi.org/10.1038/s41598-024-80449-4 |
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| author | Mariele Vertuan Júlia França da Silva Aline Dionizio Beatriz Martines de Souza Victor Mosquim Tatiana Martini Gustavo Pompermaier Garlet Samira Helena Niemeyer Marcella Esteves-Oliveira Ana Carolina Magalhães |
| author_facet | Mariele Vertuan Júlia França da Silva Aline Dionizio Beatriz Martines de Souza Victor Mosquim Tatiana Martini Gustavo Pompermaier Garlet Samira Helena Niemeyer Marcella Esteves-Oliveira Ana Carolina Magalhães |
| author_sort | Mariele Vertuan |
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| description | Abstract An animal model was applied to develop erosive tooth wear (ETW) and to evaluate the efficacy and safety of titanium tetrafluoride (TiF4) in preventing ETW. Forty-eight male Wistar rats were divided into three groups (n = 16): TiF4 (2.45% F−), NaF (2.45% F−) and placebo varnishes. Eight from each group were subjected to erosive challenges (Sprite Zero) and the other received tap water, both ad libitum. After twenty-eight days, the mandibles were resected for histopathological gingival analysis, clinical and microscopic tooth evaluation by 3D confocal laser microscopy, scanning electron microscopy (SEM-EDX) and micro-Raman spectroscopy (MRS). Organs were evaluated with respect to fluoride content. No significant difference was found in F content in tissues. No histopathological damage was seen in gingiva. ETW was clinically more aggressive in rats from placebo group consuming Sprite compared to water (Q²=12.6, p < 0.01), in accordance with confocal images. TiF4 was superior in reducing cross-section area loss (0.036 ± 0.01µm2) compared to NaF and placebo, respectively (0.044 ± 0.01/0.063 ± 0.01µm2, ANOVA, p < 0.0001). Dentin exposure was detected by SEM in rats belonging to placebo consuming Sprite. Peaks compatible with typical apatite bands were visible. TiF4 reduces the progression of ETW without causing any relevant side-effect and the rats’ model was able to simulate ETW in vivo. |
| format | Article |
| id | doaj-art-276eb97aa69c4a1ba5c6ed47c4cabed1 |
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| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-276eb97aa69c4a1ba5c6ed47c4cabed12025-08-20T02:32:53ZengNature PortfolioScientific Reports2045-23222024-11-0114111110.1038/s41598-024-80449-4Efficacy and safety of TiF4 varnish in preventing erosive tooth wear in a rat animal modelMariele Vertuan0Júlia França da Silva1Aline Dionizio2Beatriz Martines de Souza3Victor Mosquim4Tatiana Martini5Gustavo Pompermaier Garlet6Samira Helena Niemeyer7Marcella Esteves-Oliveira8Ana Carolina Magalhães9Department of Biological Sciences, Bauru School of Dentistry, University of São PauloDepartment of Biological Sciences, Bauru School of Dentistry, University of São PauloDepartment of Biological Sciences, Bauru School of Dentistry, University of São PauloDepartment of Biological Sciences, Bauru School of Dentistry, University of São PauloDepartment of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São PauloDepartment of Biological Sciences, Bauru School of Dentistry, University of São PauloDepartment of Biological Sciences, Bauru School of Dentistry, University of São PauloDepartment of Restorative, Preventive and Pediatric Dentistry, University of BernDepartment of Conservative Dentistry, Periodontology and Endodontolgy, Oral Medicine and Maxillofacial Surgery, (UZMK), University Centre of Dentistry, University of TübingenDepartment of Biological Sciences, Bauru School of Dentistry, University of São PauloAbstract An animal model was applied to develop erosive tooth wear (ETW) and to evaluate the efficacy and safety of titanium tetrafluoride (TiF4) in preventing ETW. Forty-eight male Wistar rats were divided into three groups (n = 16): TiF4 (2.45% F−), NaF (2.45% F−) and placebo varnishes. Eight from each group were subjected to erosive challenges (Sprite Zero) and the other received tap water, both ad libitum. After twenty-eight days, the mandibles were resected for histopathological gingival analysis, clinical and microscopic tooth evaluation by 3D confocal laser microscopy, scanning electron microscopy (SEM-EDX) and micro-Raman spectroscopy (MRS). Organs were evaluated with respect to fluoride content. No significant difference was found in F content in tissues. No histopathological damage was seen in gingiva. ETW was clinically more aggressive in rats from placebo group consuming Sprite compared to water (Q²=12.6, p < 0.01), in accordance with confocal images. TiF4 was superior in reducing cross-section area loss (0.036 ± 0.01µm2) compared to NaF and placebo, respectively (0.044 ± 0.01/0.063 ± 0.01µm2, ANOVA, p < 0.0001). Dentin exposure was detected by SEM in rats belonging to placebo consuming Sprite. Peaks compatible with typical apatite bands were visible. TiF4 reduces the progression of ETW without causing any relevant side-effect and the rats’ model was able to simulate ETW in vivo.https://doi.org/10.1038/s41598-024-80449-4ErosionFluorideTooth erosionTitanium |
| spellingShingle | Mariele Vertuan Júlia França da Silva Aline Dionizio Beatriz Martines de Souza Victor Mosquim Tatiana Martini Gustavo Pompermaier Garlet Samira Helena Niemeyer Marcella Esteves-Oliveira Ana Carolina Magalhães Efficacy and safety of TiF4 varnish in preventing erosive tooth wear in a rat animal model Scientific Reports Erosion Fluoride Tooth erosion Titanium |
| title | Efficacy and safety of TiF4 varnish in preventing erosive tooth wear in a rat animal model |
| title_full | Efficacy and safety of TiF4 varnish in preventing erosive tooth wear in a rat animal model |
| title_fullStr | Efficacy and safety of TiF4 varnish in preventing erosive tooth wear in a rat animal model |
| title_full_unstemmed | Efficacy and safety of TiF4 varnish in preventing erosive tooth wear in a rat animal model |
| title_short | Efficacy and safety of TiF4 varnish in preventing erosive tooth wear in a rat animal model |
| title_sort | efficacy and safety of tif4 varnish in preventing erosive tooth wear in a rat animal model |
| topic | Erosion Fluoride Tooth erosion Titanium |
| url | https://doi.org/10.1038/s41598-024-80449-4 |
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