Large-Scale AI-Based Structure and Activity Prediction Analysis of ShK Domain Peptides from Sea Anemones in the South China Sea

Sea anemone peptides represent a valuable class of biomolecules in the marine toxin library due to their various structures and functions. Among these, ShK domain peptides are particularly notable for their selective inhibition of the Kv1.3 channel, holding great potential for applications in immune...

Full description

Saved in:
Bibliographic Details
Main Authors: Ziqiang Hua, Limin Lin, Wanting Yang, Linlin Ma, Meiling Huang, Bingmiao Gao
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Marine Drugs
Subjects:
Online Access:https://www.mdpi.com/1660-3397/23/2/85
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850231775446433792
author Ziqiang Hua
Limin Lin
Wanting Yang
Linlin Ma
Meiling Huang
Bingmiao Gao
author_facet Ziqiang Hua
Limin Lin
Wanting Yang
Linlin Ma
Meiling Huang
Bingmiao Gao
author_sort Ziqiang Hua
collection DOAJ
description Sea anemone peptides represent a valuable class of biomolecules in the marine toxin library due to their various structures and functions. Among these, ShK domain peptides are particularly notable for their selective inhibition of the Kv1.3 channel, holding great potential for applications in immune regulation and the treatment of metabolic disorders. However, these peptides’ structural complexity and diversity have posed challenges for functional prediction. In this study, we compared 36 ShK domain peptides from four species of sea anemone in the South China Sea and explored their binding ability with Kv1.3 channels by combining molecular docking and dynamics simulation studies. Our findings highlight that variations in loop length, residue composition, and charge distribution among ShK domain peptides affect their binding stability and specificity. This work presents an efficient strategy for large-scale peptide structure prediction and activity screening, providing a valuable foundation for future pharmacological research.
format Article
id doaj-art-276a5e6c53a543f384b5a6d85bc680b8
institution OA Journals
issn 1660-3397
language English
publishDate 2025-02-01
publisher MDPI AG
record_format Article
series Marine Drugs
spelling doaj-art-276a5e6c53a543f384b5a6d85bc680b82025-08-20T02:03:25ZengMDPI AGMarine Drugs1660-33972025-02-012328510.3390/md23020085Large-Scale AI-Based Structure and Activity Prediction Analysis of ShK Domain Peptides from Sea Anemones in the South China SeaZiqiang Hua0Limin Lin1Wanting Yang2Linlin Ma3Meiling Huang4Bingmiao Gao5Engineering Research Center of Tropical Medicine Innovation and Transformation of Ministry of Education, Hainan Key Laboratory for Research and Development of Tropical Herbs, International Joint Research Center of Human-Machine Intelligent Collaborative for Tumor Precision Diagnosis and Treatment of Hainan Province, School of Pharmacy, Hainan Medical University, Haikou 571199, ChinaEngineering Research Center of Tropical Medicine Innovation and Transformation of Ministry of Education, Hainan Key Laboratory for Research and Development of Tropical Herbs, International Joint Research Center of Human-Machine Intelligent Collaborative for Tumor Precision Diagnosis and Treatment of Hainan Province, School of Pharmacy, Hainan Medical University, Haikou 571199, ChinaEngineering Research Center of Tropical Medicine Innovation and Transformation of Ministry of Education, Hainan Key Laboratory for Research and Development of Tropical Herbs, International Joint Research Center of Human-Machine Intelligent Collaborative for Tumor Precision Diagnosis and Treatment of Hainan Province, School of Pharmacy, Hainan Medical University, Haikou 571199, ChinaGriffith Institute for Drug Discovery (GRIDD), School of Environment and Science, Griffith University, Nathan, QLD 4111, AustraliaEngineering Research Center of Tropical Medicine Innovation and Transformation of Ministry of Education, Hainan Key Laboratory for Research and Development of Tropical Herbs, International Joint Research Center of Human-Machine Intelligent Collaborative for Tumor Precision Diagnosis and Treatment of Hainan Province, School of Pharmacy, Hainan Medical University, Haikou 571199, ChinaEngineering Research Center of Tropical Medicine Innovation and Transformation of Ministry of Education, Hainan Key Laboratory for Research and Development of Tropical Herbs, International Joint Research Center of Human-Machine Intelligent Collaborative for Tumor Precision Diagnosis and Treatment of Hainan Province, School of Pharmacy, Hainan Medical University, Haikou 571199, ChinaSea anemone peptides represent a valuable class of biomolecules in the marine toxin library due to their various structures and functions. Among these, ShK domain peptides are particularly notable for their selective inhibition of the Kv1.3 channel, holding great potential for applications in immune regulation and the treatment of metabolic disorders. However, these peptides’ structural complexity and diversity have posed challenges for functional prediction. In this study, we compared 36 ShK domain peptides from four species of sea anemone in the South China Sea and explored their binding ability with Kv1.3 channels by combining molecular docking and dynamics simulation studies. Our findings highlight that variations in loop length, residue composition, and charge distribution among ShK domain peptides affect their binding stability and specificity. This work presents an efficient strategy for large-scale peptide structure prediction and activity screening, providing a valuable foundation for future pharmacological research.https://www.mdpi.com/1660-3397/23/2/85AlphaFoldsea anemone peptidesKv1.3 channelmodelingsimulation
spellingShingle Ziqiang Hua
Limin Lin
Wanting Yang
Linlin Ma
Meiling Huang
Bingmiao Gao
Large-Scale AI-Based Structure and Activity Prediction Analysis of ShK Domain Peptides from Sea Anemones in the South China Sea
Marine Drugs
AlphaFold
sea anemone peptides
Kv1.3 channel
modeling
simulation
title Large-Scale AI-Based Structure and Activity Prediction Analysis of ShK Domain Peptides from Sea Anemones in the South China Sea
title_full Large-Scale AI-Based Structure and Activity Prediction Analysis of ShK Domain Peptides from Sea Anemones in the South China Sea
title_fullStr Large-Scale AI-Based Structure and Activity Prediction Analysis of ShK Domain Peptides from Sea Anemones in the South China Sea
title_full_unstemmed Large-Scale AI-Based Structure and Activity Prediction Analysis of ShK Domain Peptides from Sea Anemones in the South China Sea
title_short Large-Scale AI-Based Structure and Activity Prediction Analysis of ShK Domain Peptides from Sea Anemones in the South China Sea
title_sort large scale ai based structure and activity prediction analysis of shk domain peptides from sea anemones in the south china sea
topic AlphaFold
sea anemone peptides
Kv1.3 channel
modeling
simulation
url https://www.mdpi.com/1660-3397/23/2/85
work_keys_str_mv AT ziqianghua largescaleaibasedstructureandactivitypredictionanalysisofshkdomainpeptidesfromseaanemonesinthesouthchinasea
AT liminlin largescaleaibasedstructureandactivitypredictionanalysisofshkdomainpeptidesfromseaanemonesinthesouthchinasea
AT wantingyang largescaleaibasedstructureandactivitypredictionanalysisofshkdomainpeptidesfromseaanemonesinthesouthchinasea
AT linlinma largescaleaibasedstructureandactivitypredictionanalysisofshkdomainpeptidesfromseaanemonesinthesouthchinasea
AT meilinghuang largescaleaibasedstructureandactivitypredictionanalysisofshkdomainpeptidesfromseaanemonesinthesouthchinasea
AT bingmiaogao largescaleaibasedstructureandactivitypredictionanalysisofshkdomainpeptidesfromseaanemonesinthesouthchinasea