Characterization of the Antibiotic Compound No. 70 Produced by Streptomyces sp. IMV-70
We describe the actinomycete strain IMV-70 isolated from the soils of Kazakhstan, which produces potent antibiotics with high levels of antibacterial activity. After the research of its morphological, chemotaxonomic, and cultural characteristics, the strain with potential to be developed further as...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/594231 |
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| author | Lyudmila P. Trenozhnikova Almagul K. Khasenova Assya S. Balgimbaeva Galina B. Fedorova Genrikh S. Katrukha Nina L. Tokareva Boo H. Kwa Azliyati Azizan |
| author_facet | Lyudmila P. Trenozhnikova Almagul K. Khasenova Assya S. Balgimbaeva Galina B. Fedorova Genrikh S. Katrukha Nina L. Tokareva Boo H. Kwa Azliyati Azizan |
| author_sort | Lyudmila P. Trenozhnikova |
| collection | DOAJ |
| description | We describe the actinomycete strain IMV-70 isolated from the soils of Kazakhstan, which produces potent antibiotics with high levels of antibacterial activity. After the research of its morphological, chemotaxonomic, and cultural characteristics, the strain with potential to be developed further as a novel class of antibiotics with chemotherapeutics potential was identified as Streptomyces sp. IMV-70. In the process of fermentation, the strain Streptomyces spp. IMV-70 produces the antibiotic no. 70, which was isolated from the culture broth by extraction with organic solvents. Antibiotic compound no. 70 was purified and separated into individual components by HPLC, TLC, and column chromatography methods. The main component of the compound is the antibiotic 70-A, which was found to be identical to the peptolide etamycin A. Two other antibiotics 70-B and 70-C have never been described and therefore are new antibiotics. The physical-chemical and biological characteristics of these preparations were described and further researched. Determination of the optimal growth conditions to cultivate actinomycete-producer strain IMV-70 and development of methods to isolate, purify, and accumulate preparations of the new antibiotic no. 70 enable us to research further the potential of this new class of antibiotics. |
| format | Article |
| id | doaj-art-27696fe97d5c484c828bd6d89f5cc1c1 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-27696fe97d5c484c828bd6d89f5cc1c12025-08-20T03:54:57ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/594231594231Characterization of the Antibiotic Compound No. 70 Produced by Streptomyces sp. IMV-70Lyudmila P. Trenozhnikova0Almagul K. Khasenova1Assya S. Balgimbaeva2Galina B. Fedorova3Genrikh S. Katrukha4Nina L. Tokareva5Boo H. Kwa6Azliyati Azizan7Institute of Microbiology and Virology, Ministry of Education and Science Committee, 103, Bogenbay batyr Street, Almaty, KazakhstanInstitute of Microbiology and Virology, Ministry of Education and Science Committee, 103, Bogenbay batyr Street, Almaty, KazakhstanInstitute of Microbiology and Virology, Ministry of Education and Science Committee, 103, Bogenbay batyr Street, Almaty, KazakhstanResearch Institute of New Antibiotics, Russian Academy of Medical Sciences, Moscow, RussiaResearch Institute of New Antibiotics, Russian Academy of Medical Sciences, Moscow, RussiaResearch Institute of New Antibiotics, Russian Academy of Medical Sciences, Moscow, RussiaGlobal Health Department, College of Public Health, 13201 Bruce B. Downs Boulevard, Tampa, FL 33612, USAGlobal Health Department, College of Public Health, 13201 Bruce B. Downs Boulevard, Tampa, FL 33612, USAWe describe the actinomycete strain IMV-70 isolated from the soils of Kazakhstan, which produces potent antibiotics with high levels of antibacterial activity. After the research of its morphological, chemotaxonomic, and cultural characteristics, the strain with potential to be developed further as a novel class of antibiotics with chemotherapeutics potential was identified as Streptomyces sp. IMV-70. In the process of fermentation, the strain Streptomyces spp. IMV-70 produces the antibiotic no. 70, which was isolated from the culture broth by extraction with organic solvents. Antibiotic compound no. 70 was purified and separated into individual components by HPLC, TLC, and column chromatography methods. The main component of the compound is the antibiotic 70-A, which was found to be identical to the peptolide etamycin A. Two other antibiotics 70-B and 70-C have never been described and therefore are new antibiotics. The physical-chemical and biological characteristics of these preparations were described and further researched. Determination of the optimal growth conditions to cultivate actinomycete-producer strain IMV-70 and development of methods to isolate, purify, and accumulate preparations of the new antibiotic no. 70 enable us to research further the potential of this new class of antibiotics.http://dx.doi.org/10.1100/2012/594231 |
| spellingShingle | Lyudmila P. Trenozhnikova Almagul K. Khasenova Assya S. Balgimbaeva Galina B. Fedorova Genrikh S. Katrukha Nina L. Tokareva Boo H. Kwa Azliyati Azizan Characterization of the Antibiotic Compound No. 70 Produced by Streptomyces sp. IMV-70 The Scientific World Journal |
| title | Characterization of the Antibiotic Compound No. 70 Produced by Streptomyces sp. IMV-70 |
| title_full | Characterization of the Antibiotic Compound No. 70 Produced by Streptomyces sp. IMV-70 |
| title_fullStr | Characterization of the Antibiotic Compound No. 70 Produced by Streptomyces sp. IMV-70 |
| title_full_unstemmed | Characterization of the Antibiotic Compound No. 70 Produced by Streptomyces sp. IMV-70 |
| title_short | Characterization of the Antibiotic Compound No. 70 Produced by Streptomyces sp. IMV-70 |
| title_sort | characterization of the antibiotic compound no 70 produced by streptomyces sp imv 70 |
| url | http://dx.doi.org/10.1100/2012/594231 |
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