Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain
This study set out to investigate the effect of massage on the Toll-like receptor 4 (TLR4) signalling pathway in the dorsal root ganglia of rats that had undergone spinal nerve ligation (SNL), with the hypothesis that massage could be used as an analgesic. Forty female SD rats were randomly divided...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Pain Research and Management |
| Online Access: | http://dx.doi.org/10.1155/2020/8309745 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546929400086528 |
|---|---|
| author | Qian Wang Jing Lin Peng Yang Yingye Liang Dongming Lu Kailong Wang Wei Gan Jianping Fu Zhenbao Gan Mingchen Ma Pingting Wu Fengshi He Jun Pang Hongliang Tang |
| author_facet | Qian Wang Jing Lin Peng Yang Yingye Liang Dongming Lu Kailong Wang Wei Gan Jianping Fu Zhenbao Gan Mingchen Ma Pingting Wu Fengshi He Jun Pang Hongliang Tang |
| author_sort | Qian Wang |
| collection | DOAJ |
| description | This study set out to investigate the effect of massage on the Toll-like receptor 4 (TLR4) signalling pathway in the dorsal root ganglia of rats that had undergone spinal nerve ligation (SNL), with the hypothesis that massage could be used as an analgesic. Forty female SD rats were randomly divided into 5 groups: the control group, sham-operated group, model group, sham massage group, and massage group. There were 8 rats in each group. SNL rat models were established in the model group, sham massage group, and massage group. Rats in the sham-operated group underwent surgery to expose the vertebral nerves, but no further procedures were performed. The control group consisted of intact animals. The rats in the massage group underwent massage using a massage simulation machine once a day for 14 d in succession; the hind limbs of the rats in the sham massage group were gently touched with a cloth bag once a day for 14 continuous days. The rats in the control group, the sham-operated group, and the model group did not receive any intervention and were observed for 14 d. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) of rats in each group were detected 1 d before modelling and at 1, 3, 7, and 14 d after modelling. Fourteen days after modelling, the expression levels of TLR4, IRAK1, TRAF6, TNF-α, and IL-6 were detected in all rats. The PWTL and PWMT of SNL rats were decreased, while these parameters were elevated after massage. SNL rats showed higher levels of TLR4, IRAK1, TRAF6, IL-6, and TNF-α, and massage effectively lowered the expression levels of these molecules. Inhibiting activation of the TLR4 signalling pathway, which can reduce the release of inflammatory factors, may be one mechanism by which massage treats neuropathic pain. |
| format | Article |
| id | doaj-art-2761a8d92dd34adf80300eb21bcf0bc8 |
| institution | Kabale University |
| issn | 1203-6765 1918-1523 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pain Research and Management |
| spelling | doaj-art-2761a8d92dd34adf80300eb21bcf0bc82025-02-03T06:46:30ZengWileyPain Research and Management1203-67651918-15232020-01-01202010.1155/2020/83097458309745Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic PainQian Wang0Jing Lin1Peng Yang2Yingye Liang3Dongming Lu4Kailong Wang5Wei Gan6Jianping Fu7Zhenbao Gan8Mingchen Ma9Pingting Wu10Fengshi He11Jun Pang12Hongliang Tang13Department of Acupuncture, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530000, ChinaDepartment of Acupuncture, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530000, ChinaGuangxi University of Chinese Medicine, Nanning, Guangxi 530200, ChinaDepartment of Massage, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530000, ChinaDepartment of Massage, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530000, ChinaDepartment of Massage, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530000, ChinaDepartment of Massage, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530000, ChinaDepartment of Massage, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530000, ChinaDepartment of Massage, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530000, ChinaGuangxi University of Chinese Medicine, Nanning, Guangxi 530200, ChinaGuangxi University of Chinese Medicine, Nanning, Guangxi 530200, ChinaGuangxi University of Chinese Medicine, Nanning, Guangxi 530200, ChinaDepartment of Massage, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530000, ChinaDepartment of Massage, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530000, ChinaThis study set out to investigate the effect of massage on the Toll-like receptor 4 (TLR4) signalling pathway in the dorsal root ganglia of rats that had undergone spinal nerve ligation (SNL), with the hypothesis that massage could be used as an analgesic. Forty female SD rats were randomly divided into 5 groups: the control group, sham-operated group, model group, sham massage group, and massage group. There were 8 rats in each group. SNL rat models were established in the model group, sham massage group, and massage group. Rats in the sham-operated group underwent surgery to expose the vertebral nerves, but no further procedures were performed. The control group consisted of intact animals. The rats in the massage group underwent massage using a massage simulation machine once a day for 14 d in succession; the hind limbs of the rats in the sham massage group were gently touched with a cloth bag once a day for 14 continuous days. The rats in the control group, the sham-operated group, and the model group did not receive any intervention and were observed for 14 d. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) of rats in each group were detected 1 d before modelling and at 1, 3, 7, and 14 d after modelling. Fourteen days after modelling, the expression levels of TLR4, IRAK1, TRAF6, TNF-α, and IL-6 were detected in all rats. The PWTL and PWMT of SNL rats were decreased, while these parameters were elevated after massage. SNL rats showed higher levels of TLR4, IRAK1, TRAF6, IL-6, and TNF-α, and massage effectively lowered the expression levels of these molecules. Inhibiting activation of the TLR4 signalling pathway, which can reduce the release of inflammatory factors, may be one mechanism by which massage treats neuropathic pain.http://dx.doi.org/10.1155/2020/8309745 |
| spellingShingle | Qian Wang Jing Lin Peng Yang Yingye Liang Dongming Lu Kailong Wang Wei Gan Jianping Fu Zhenbao Gan Mingchen Ma Pingting Wu Fengshi He Jun Pang Hongliang Tang Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain Pain Research and Management |
| title | Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain |
| title_full | Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain |
| title_fullStr | Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain |
| title_full_unstemmed | Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain |
| title_short | Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain |
| title_sort | effect of massage on the tlr4 signalling pathway in rats with neuropathic pain |
| url | http://dx.doi.org/10.1155/2020/8309745 |
| work_keys_str_mv | AT qianwang effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT jinglin effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT pengyang effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT yingyeliang effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT dongminglu effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT kailongwang effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT weigan effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT jianpingfu effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT zhenbaogan effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT mingchenma effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT pingtingwu effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT fengshihe effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT junpang effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain AT hongliangtang effectofmassageonthetlr4signallingpathwayinratswithneuropathicpain |