In vivo Outcomes of the Application of Stem Cell-derived Conditioned Medium (Secretome) or Its Derivatives toward Skin Flap Following Ischemia–Reperfusion Injury: A Systematic Review of Experimental Studies
Skin flaps are tissue transferred from a donor to a recipient site to fill covering defects in the surface of the body. During transfer, skin flaps are severely predisposed to ischemia not only due to ischemia but also to ischemia–reperfusion (I/R) injury, a paradoxical exacerbation of cellular deat...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-01-01
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| Series: | Turkish Journal of Plastic Surgery |
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| Online Access: | https://journals.lww.com/10.4103/tjps.tjps_20_24 |
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| author | Richard Christian Suteja I. Gusti Ayu Maha Hiranandini Prawista I. Komang Hotra Adiputra Giovanca Verentzia Purnama Darren Junior I. Gusti Ngurah Ariestha Satya Diksha I. Gusti Ngurah Dinda Pratyaksa I. Dewa Gede Agung Siwa Guptha Novea Indratmo Albert Salim I. Putu Divanaya Suryanov Gede Wara Samsarga |
| author_facet | Richard Christian Suteja I. Gusti Ayu Maha Hiranandini Prawista I. Komang Hotra Adiputra Giovanca Verentzia Purnama Darren Junior I. Gusti Ngurah Ariestha Satya Diksha I. Gusti Ngurah Dinda Pratyaksa I. Dewa Gede Agung Siwa Guptha Novea Indratmo Albert Salim I. Putu Divanaya Suryanov Gede Wara Samsarga |
| author_sort | Richard Christian Suteja |
| collection | DOAJ |
| description | Skin flaps are tissue transferred from a donor to a recipient site to fill covering defects in the surface of the body. During transfer, skin flaps are severely predisposed to ischemia not only due to ischemia but also to ischemia–reperfusion (I/R) injury, a paradoxical exacerbation of cellular death following blood flow restoration in tissues exposed to ischemic insults. This systematic review aimed to report the in vivo outcomes of the application of stem cell-derived conditioned medium (secretome) or its derivatives toward skin flap following I/R injury in experimental studies. This study was a systematic review with literature searched using Science Direct, PubMed, Directory of Open Access Journal, Cochrane Central, Clinicaltrials.gov, and Europe PMC on January 24, 2024, for studies reporting the effects of stem cell-derived conditioned medium (secretome) or its derivatives in the skin flap I/R injury in animal models. We obtained a total of 318 studies during the search, which were then screened. Ten studies were included in the review. Our study found that stem cell-conditioned medium increases overall flap survivability; promotes vascularization, perfusion, and angiogenesis; reduces inflammation, reduces apoptosis and oxidants; and increases collagen secretion and maturation. The effect was found to be even more profound when the stem cells were set to proliferate under hypoxic conditions. Secretome did not only exhibit prosurvival and proproliferation properties suitable for responding to ischemic insults but also anti-inflammatory and antiapoptotic properties suitable for responding to I/R injury. Furthermore, secretome also assisted native structure recovery. The results from the experimental studies found that secretome is a promising therapy to increase flap survivability when also promoting histology recovery. |
| format | Article |
| id | doaj-art-27530d4bf9864311bf3d9be509d9d3fc |
| institution | OA Journals |
| issn | 2528-8644 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Turkish Journal of Plastic Surgery |
| spelling | doaj-art-27530d4bf9864311bf3d9be509d9d3fc2025-08-20T02:37:57ZengWolters Kluwer Medknow PublicationsTurkish Journal of Plastic Surgery2528-86442025-01-0133111610.4103/tjps.tjps_20_24In vivo Outcomes of the Application of Stem Cell-derived Conditioned Medium (Secretome) or Its Derivatives toward Skin Flap Following Ischemia–Reperfusion Injury: A Systematic Review of Experimental StudiesRichard Christian SutejaI. Gusti Ayu Maha Hiranandini PrawistaI. Komang Hotra AdiputraGiovanca Verentzia PurnamaDarren JuniorI. Gusti Ngurah Ariestha Satya DikshaI. Gusti Ngurah Dinda PratyaksaI. Dewa Gede Agung Siwa GupthaNovea IndratmoAlbert SalimI. Putu Divanaya SuryanovGede Wara SamsargaSkin flaps are tissue transferred from a donor to a recipient site to fill covering defects in the surface of the body. During transfer, skin flaps are severely predisposed to ischemia not only due to ischemia but also to ischemia–reperfusion (I/R) injury, a paradoxical exacerbation of cellular death following blood flow restoration in tissues exposed to ischemic insults. This systematic review aimed to report the in vivo outcomes of the application of stem cell-derived conditioned medium (secretome) or its derivatives toward skin flap following I/R injury in experimental studies. This study was a systematic review with literature searched using Science Direct, PubMed, Directory of Open Access Journal, Cochrane Central, Clinicaltrials.gov, and Europe PMC on January 24, 2024, for studies reporting the effects of stem cell-derived conditioned medium (secretome) or its derivatives in the skin flap I/R injury in animal models. We obtained a total of 318 studies during the search, which were then screened. Ten studies were included in the review. Our study found that stem cell-conditioned medium increases overall flap survivability; promotes vascularization, perfusion, and angiogenesis; reduces inflammation, reduces apoptosis and oxidants; and increases collagen secretion and maturation. The effect was found to be even more profound when the stem cells were set to proliferate under hypoxic conditions. Secretome did not only exhibit prosurvival and proproliferation properties suitable for responding to ischemic insults but also anti-inflammatory and antiapoptotic properties suitable for responding to I/R injury. Furthermore, secretome also assisted native structure recovery. The results from the experimental studies found that secretome is a promising therapy to increase flap survivability when also promoting histology recovery.https://journals.lww.com/10.4103/tjps.tjps_20_24conditioned mediumischemia–reperfusion injuryregenerative medicinesecretomeskin flapstem cellsystematic review |
| spellingShingle | Richard Christian Suteja I. Gusti Ayu Maha Hiranandini Prawista I. Komang Hotra Adiputra Giovanca Verentzia Purnama Darren Junior I. Gusti Ngurah Ariestha Satya Diksha I. Gusti Ngurah Dinda Pratyaksa I. Dewa Gede Agung Siwa Guptha Novea Indratmo Albert Salim I. Putu Divanaya Suryanov Gede Wara Samsarga In vivo Outcomes of the Application of Stem Cell-derived Conditioned Medium (Secretome) or Its Derivatives toward Skin Flap Following Ischemia–Reperfusion Injury: A Systematic Review of Experimental Studies Turkish Journal of Plastic Surgery conditioned medium ischemia–reperfusion injury regenerative medicine secretome skin flap stem cell systematic review |
| title | In vivo Outcomes of the Application of Stem Cell-derived Conditioned Medium (Secretome) or Its Derivatives toward Skin Flap Following Ischemia–Reperfusion Injury: A Systematic Review of Experimental Studies |
| title_full | In vivo Outcomes of the Application of Stem Cell-derived Conditioned Medium (Secretome) or Its Derivatives toward Skin Flap Following Ischemia–Reperfusion Injury: A Systematic Review of Experimental Studies |
| title_fullStr | In vivo Outcomes of the Application of Stem Cell-derived Conditioned Medium (Secretome) or Its Derivatives toward Skin Flap Following Ischemia–Reperfusion Injury: A Systematic Review of Experimental Studies |
| title_full_unstemmed | In vivo Outcomes of the Application of Stem Cell-derived Conditioned Medium (Secretome) or Its Derivatives toward Skin Flap Following Ischemia–Reperfusion Injury: A Systematic Review of Experimental Studies |
| title_short | In vivo Outcomes of the Application of Stem Cell-derived Conditioned Medium (Secretome) or Its Derivatives toward Skin Flap Following Ischemia–Reperfusion Injury: A Systematic Review of Experimental Studies |
| title_sort | in vivo outcomes of the application of stem cell derived conditioned medium secretome or its derivatives toward skin flap following ischemia reperfusion injury a systematic review of experimental studies |
| topic | conditioned medium ischemia–reperfusion injury regenerative medicine secretome skin flap stem cell systematic review |
| url | https://journals.lww.com/10.4103/tjps.tjps_20_24 |
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