Protocol for developing shape-morphing 4D bioprinted magnetic constructs to promote articular cartilage regeneration using silk fibroin-gelatin bioink
Summary: External magnetic fields can regulate cellular responses. Here, we present a protocol to fabricate magnetic constructs by 4D bioprinting with shape-morphing properties using silk fibroin-gelatin bioinks for articular cartilage regeneration. We illustrate the steps for magnetic bioink formul...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724004970 |
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| author | Juhi Chakraborty Chandrashish Roy Maria Kalogeropoulou Carlos Mota Sourabh Ghosh Lorenzo Moroni |
| author_facet | Juhi Chakraborty Chandrashish Roy Maria Kalogeropoulou Carlos Mota Sourabh Ghosh Lorenzo Moroni |
| author_sort | Juhi Chakraborty |
| collection | DOAJ |
| description | Summary: External magnetic fields can regulate cellular responses. Here, we present a protocol to fabricate magnetic constructs by 4D bioprinting with shape-morphing properties using silk fibroin-gelatin bioinks for articular cartilage regeneration. We illustrate the steps for magnetic bioink formulation, bioprinting, and chondrogenic induction of human bone marrow mesenchymal stem/stromal cells. We detail the steps to actuate the constructs using an external magnetic field and then characterize chondrogenesis. Magnetic field actuation may be helpful for mechanically activating constructs for articular cartilage regeneration.For complete details on the use and execution of this protocol, please refer to Chakraborty et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-2746c283128d4be5b01de49d4e2660bd |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-2746c283128d4be5b01de49d4e2660bd2024-11-20T05:07:50ZengElsevierSTAR Protocols2666-16672024-12-0154103332Protocol for developing shape-morphing 4D bioprinted magnetic constructs to promote articular cartilage regeneration using silk fibroin-gelatin bioinkJuhi Chakraborty0Chandrashish Roy1Maria Kalogeropoulou2Carlos Mota3Sourabh Ghosh4Lorenzo Moroni5Department of Textile and Fibre Engineering, Indian Institute of Technology Delhi, New Delhi 110016, IndiaDepartment of Textile and Fibre Engineering, Indian Institute of Technology Delhi, New Delhi 110016, IndiaDepartment of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6211 LK Maastricht, the NetherlandsDepartment of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6211 LK Maastricht, the NetherlandsDepartment of Textile and Fibre Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India; Corresponding authorDepartment of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, 6211 LK Maastricht, the Netherlands; Corresponding authorSummary: External magnetic fields can regulate cellular responses. Here, we present a protocol to fabricate magnetic constructs by 4D bioprinting with shape-morphing properties using silk fibroin-gelatin bioinks for articular cartilage regeneration. We illustrate the steps for magnetic bioink formulation, bioprinting, and chondrogenic induction of human bone marrow mesenchymal stem/stromal cells. We detail the steps to actuate the constructs using an external magnetic field and then characterize chondrogenesis. Magnetic field actuation may be helpful for mechanically activating constructs for articular cartilage regeneration.For complete details on the use and execution of this protocol, please refer to Chakraborty et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724004970Stem CellsTissue EngineeringMaterial sciences |
| spellingShingle | Juhi Chakraborty Chandrashish Roy Maria Kalogeropoulou Carlos Mota Sourabh Ghosh Lorenzo Moroni Protocol for developing shape-morphing 4D bioprinted magnetic constructs to promote articular cartilage regeneration using silk fibroin-gelatin bioink STAR Protocols Stem Cells Tissue Engineering Material sciences |
| title | Protocol for developing shape-morphing 4D bioprinted magnetic constructs to promote articular cartilage regeneration using silk fibroin-gelatin bioink |
| title_full | Protocol for developing shape-morphing 4D bioprinted magnetic constructs to promote articular cartilage regeneration using silk fibroin-gelatin bioink |
| title_fullStr | Protocol for developing shape-morphing 4D bioprinted magnetic constructs to promote articular cartilage regeneration using silk fibroin-gelatin bioink |
| title_full_unstemmed | Protocol for developing shape-morphing 4D bioprinted magnetic constructs to promote articular cartilage regeneration using silk fibroin-gelatin bioink |
| title_short | Protocol for developing shape-morphing 4D bioprinted magnetic constructs to promote articular cartilage regeneration using silk fibroin-gelatin bioink |
| title_sort | protocol for developing shape morphing 4d bioprinted magnetic constructs to promote articular cartilage regeneration using silk fibroin gelatin bioink |
| topic | Stem Cells Tissue Engineering Material sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724004970 |
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