Shengxian decoction alleviates cyclophosphamide-induced immunosuppression via improving B cell-mediated immune responses
BackgroundChemotherapy is a prevalent and extensively utilized cancer treatment modality. However, it can result in immunosuppression. Shengxian Decoction (SXD) is a Traditional Chinese Medicine formula that has been demonstrated to improve immunosuppression caused by chemotherapy in clinical settin...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1565451/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850201142812737536 |
|---|---|
| author | Chengyu Yang Chengyu Yang Ya Xu Ya Xu Fenfang Gong Fenfang Gong Silu Li Silu Li Huang Zhan Huang Zhan Zhixuan Huang Zhixuan Huang Maolin Wang Maolin Wang Hui Li Hui Li Hui Li Hengjun Huang Hengjun Huang |
| author_facet | Chengyu Yang Chengyu Yang Ya Xu Ya Xu Fenfang Gong Fenfang Gong Silu Li Silu Li Huang Zhan Huang Zhan Zhixuan Huang Zhixuan Huang Maolin Wang Maolin Wang Hui Li Hui Li Hui Li Hengjun Huang Hengjun Huang |
| author_sort | Chengyu Yang |
| collection | DOAJ |
| description | BackgroundChemotherapy is a prevalent and extensively utilized cancer treatment modality. However, it can result in immunosuppression. Shengxian Decoction (SXD) is a Traditional Chinese Medicine formula that has been demonstrated to improve immunosuppression caused by chemotherapy in clinical settings. Nevertheless, the therapeutic evaluation and mechanism of SXD in regulating immunosuppression remain unclear. The aim of this study was to ascertain the efficacy of SXD in immunocompromised mice and to elucidate the underlying immunological mechanisms.MethodsThe immunosuppression mouse model was generated through the administration of cyclophosphamide for 3 days. After that, the mice were treated by SXD extracts at doses of 0.47 and 0.94 g/kg/day, respectively. The spleen, thymus and blood of mice were collected for evaluation of drug efficacy. The population of B and T cells was detected by flow cytometry. The genes regulated by SXD in spleen were identified by utilizing RNA sequencing. The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment were used to analyze the signaling pathway modulated by SXD. The protein expression of B cell transcription factors was detected by the immunoblotting. The signaling pathways modulated by SXD were elucidated using transcriptomics analysis complemented by network analysis.ResultsSXD treatment ameliorates decreased splenic and thymic organ indexes, as well as decreased lymphocyte number in the spleen in immunosuppressed mice. ELISA assay and flow cytometry shows that SXD promotes attenuate B-cell-mediated humoral immune responses. The RNA sequencing reveals that SXD primarily upregulates the B cell-mediated immune response and B cell receptor signaling pathway. SXD treatment upregulates the expression of B cell differentiation factors Pax5, Tcf3 and promotes splenic cell proliferation. Furthermore, SXD extract significantly inhibits hypoxia and senescence pathways in the spleen.ConclusionSXD exerts a protective effect against CTX-induced immunosuppression by upregulating B cell immunity and inhibiting hypoxia and senescence pathways. |
| format | Article |
| id | doaj-art-2741d12e1f4e42908a765d6017b92d76 |
| institution | OA Journals |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-2741d12e1f4e42908a765d6017b92d762025-08-20T02:12:06ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-04-011610.3389/fphar.2025.15654511565451Shengxian decoction alleviates cyclophosphamide-induced immunosuppression via improving B cell-mediated immune responsesChengyu Yang0Chengyu Yang1Ya Xu2Ya Xu3Fenfang Gong4Fenfang Gong5Silu Li6Silu Li7Huang Zhan8Huang Zhan9Zhixuan Huang10Zhixuan Huang11Maolin Wang12Maolin Wang13Hui Li14Hui Li15Hui Li16Hengjun Huang17Hengjun Huang18Jiangxi Province Key Laboratory of Traditional Chinese Medicine Pharmacology, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences, Nanchang, ChinaJiangxi Health Industry Institute of Traditional Chinese Medicine, Nanchang, ChinaJiangxi Province Key Laboratory of Traditional Chinese Medicine Pharmacology, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences, Nanchang, ChinaJiangxi Health Industry Institute of Traditional Chinese Medicine, Nanchang, ChinaJiangxi Province Key Laboratory of Traditional Chinese Medicine Pharmacology, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences, Nanchang, ChinaJiangxi Health Industry Institute of Traditional Chinese Medicine, Nanchang, ChinaJiangxi Province Key Laboratory of Traditional Chinese Medicine Pharmacology, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences, Nanchang, ChinaJiangxi Health Industry Institute of Traditional Chinese Medicine, Nanchang, ChinaJiangxi Province Key Laboratory of Traditional Chinese Medicine Pharmacology, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences, Nanchang, ChinaJiangxi Health Industry Institute of Traditional Chinese Medicine, Nanchang, ChinaJiangxi Province Key Laboratory of Traditional Chinese Medicine Pharmacology, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences, Nanchang, ChinaJiangxi Health Industry Institute of Traditional Chinese Medicine, Nanchang, ChinaJiangxi Province Key Laboratory of Traditional Chinese Medicine Pharmacology, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences, Nanchang, ChinaJiangxi Health Industry Institute of Traditional Chinese Medicine, Nanchang, ChinaJiangxi Province Key Laboratory of Traditional Chinese Medicine Pharmacology, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences, Nanchang, ChinaJiangxi Health Industry Institute of Traditional Chinese Medicine, Nanchang, ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaJiangxi Province Key Laboratory of Traditional Chinese Medicine Pharmacology, Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences, Nanchang, ChinaJiangxi Health Industry Institute of Traditional Chinese Medicine, Nanchang, ChinaBackgroundChemotherapy is a prevalent and extensively utilized cancer treatment modality. However, it can result in immunosuppression. Shengxian Decoction (SXD) is a Traditional Chinese Medicine formula that has been demonstrated to improve immunosuppression caused by chemotherapy in clinical settings. Nevertheless, the therapeutic evaluation and mechanism of SXD in regulating immunosuppression remain unclear. The aim of this study was to ascertain the efficacy of SXD in immunocompromised mice and to elucidate the underlying immunological mechanisms.MethodsThe immunosuppression mouse model was generated through the administration of cyclophosphamide for 3 days. After that, the mice were treated by SXD extracts at doses of 0.47 and 0.94 g/kg/day, respectively. The spleen, thymus and blood of mice were collected for evaluation of drug efficacy. The population of B and T cells was detected by flow cytometry. The genes regulated by SXD in spleen were identified by utilizing RNA sequencing. The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment were used to analyze the signaling pathway modulated by SXD. The protein expression of B cell transcription factors was detected by the immunoblotting. The signaling pathways modulated by SXD were elucidated using transcriptomics analysis complemented by network analysis.ResultsSXD treatment ameliorates decreased splenic and thymic organ indexes, as well as decreased lymphocyte number in the spleen in immunosuppressed mice. ELISA assay and flow cytometry shows that SXD promotes attenuate B-cell-mediated humoral immune responses. The RNA sequencing reveals that SXD primarily upregulates the B cell-mediated immune response and B cell receptor signaling pathway. SXD treatment upregulates the expression of B cell differentiation factors Pax5, Tcf3 and promotes splenic cell proliferation. Furthermore, SXD extract significantly inhibits hypoxia and senescence pathways in the spleen.ConclusionSXD exerts a protective effect against CTX-induced immunosuppression by upregulating B cell immunity and inhibiting hypoxia and senescence pathways.https://www.frontiersin.org/articles/10.3389/fphar.2025.1565451/fullShengxian decoctioncyclophosphamideimmunosuppressionB cellhumoral immune responses |
| spellingShingle | Chengyu Yang Chengyu Yang Ya Xu Ya Xu Fenfang Gong Fenfang Gong Silu Li Silu Li Huang Zhan Huang Zhan Zhixuan Huang Zhixuan Huang Maolin Wang Maolin Wang Hui Li Hui Li Hui Li Hengjun Huang Hengjun Huang Shengxian decoction alleviates cyclophosphamide-induced immunosuppression via improving B cell-mediated immune responses Frontiers in Pharmacology Shengxian decoction cyclophosphamide immunosuppression B cell humoral immune responses |
| title | Shengxian decoction alleviates cyclophosphamide-induced immunosuppression via improving B cell-mediated immune responses |
| title_full | Shengxian decoction alleviates cyclophosphamide-induced immunosuppression via improving B cell-mediated immune responses |
| title_fullStr | Shengxian decoction alleviates cyclophosphamide-induced immunosuppression via improving B cell-mediated immune responses |
| title_full_unstemmed | Shengxian decoction alleviates cyclophosphamide-induced immunosuppression via improving B cell-mediated immune responses |
| title_short | Shengxian decoction alleviates cyclophosphamide-induced immunosuppression via improving B cell-mediated immune responses |
| title_sort | shengxian decoction alleviates cyclophosphamide induced immunosuppression via improving b cell mediated immune responses |
| topic | Shengxian decoction cyclophosphamide immunosuppression B cell humoral immune responses |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1565451/full |
| work_keys_str_mv | AT chengyuyang shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT chengyuyang shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT yaxu shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT yaxu shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT fenfanggong shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT fenfanggong shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT siluli shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT siluli shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT huangzhan shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT huangzhan shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT zhixuanhuang shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT zhixuanhuang shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT maolinwang shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT maolinwang shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT huili shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT huili shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT huili shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT hengjunhuang shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses AT hengjunhuang shengxiandecoctionalleviatescyclophosphamideinducedimmunosuppressionviaimprovingbcellmediatedimmuneresponses |