Pathogenicity of Treponema denticola Wild-Type and Mutant Strain Tested by an Active Mode of Periodontal Infection Using Microinjection
The available passive mode of periodontal infections in mice requires high efficiency of bacterial attachment and invasiveness and is not always suitable to test the pathogenicity of genetically engineered mutant strains. We developed an active mode of oral infection, using microinjection in the mar...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2012/549169 |
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| _version_ | 1849306779509325824 |
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| author | Jacques Izard Hajime Sasaki Ralph Kent |
| author_facet | Jacques Izard Hajime Sasaki Ralph Kent |
| author_sort | Jacques Izard |
| collection | DOAJ |
| description | The available passive mode of periodontal infections in mice requires high efficiency of bacterial attachment and invasiveness and is not always suitable to test the pathogenicity of genetically engineered mutant strains. We developed an active mode of oral infection, using microinjection in the marginal gingiva of mice, to test the pathogenicity of a genetically engineered Treponema denticola mutant strain deficient in intermediate-like filaments, compared to the wild-type strain. This targeted mode of infection inoculates the bacterial strain to be tested directly at a lesion site (needle entry point) located at the future periodontal lesion site. The efficiency of T. denticola wild-type strain to elicit bone loss contrasted with the lack of pathogenicity of the intermediate-like filament deficient mutant strain in comparison to the sham infection. The periodontal microinjection oral model in mice can be used for a variety of applications complementary to the passive mode of periodontal infection in context of pathogenicity testing. |
| format | Article |
| id | doaj-art-273d19a41bba4393b43871d41d3174f1 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-273d19a41bba4393b43871d41d3174f12025-08-20T03:54:57ZengWileyInternational Journal of Dentistry1687-87281687-87362012-01-01201210.1155/2012/549169549169Pathogenicity of Treponema denticola Wild-Type and Mutant Strain Tested by an Active Mode of Periodontal Infection Using MicroinjectionJacques Izard0Hajime Sasaki1Ralph Kent2Department of Molecular Genetics, The Forsyth Institute, Cambridge, MA 02142, USADepartment of Molecular Genetics, The Forsyth Institute, Cambridge, MA 02142, USADepartment of Molecular Genetics, The Forsyth Institute, Cambridge, MA 02142, USAThe available passive mode of periodontal infections in mice requires high efficiency of bacterial attachment and invasiveness and is not always suitable to test the pathogenicity of genetically engineered mutant strains. We developed an active mode of oral infection, using microinjection in the marginal gingiva of mice, to test the pathogenicity of a genetically engineered Treponema denticola mutant strain deficient in intermediate-like filaments, compared to the wild-type strain. This targeted mode of infection inoculates the bacterial strain to be tested directly at a lesion site (needle entry point) located at the future periodontal lesion site. The efficiency of T. denticola wild-type strain to elicit bone loss contrasted with the lack of pathogenicity of the intermediate-like filament deficient mutant strain in comparison to the sham infection. The periodontal microinjection oral model in mice can be used for a variety of applications complementary to the passive mode of periodontal infection in context of pathogenicity testing.http://dx.doi.org/10.1155/2012/549169 |
| spellingShingle | Jacques Izard Hajime Sasaki Ralph Kent Pathogenicity of Treponema denticola Wild-Type and Mutant Strain Tested by an Active Mode of Periodontal Infection Using Microinjection International Journal of Dentistry |
| title | Pathogenicity of Treponema denticola Wild-Type and Mutant Strain Tested by an Active Mode of Periodontal Infection Using Microinjection |
| title_full | Pathogenicity of Treponema denticola Wild-Type and Mutant Strain Tested by an Active Mode of Periodontal Infection Using Microinjection |
| title_fullStr | Pathogenicity of Treponema denticola Wild-Type and Mutant Strain Tested by an Active Mode of Periodontal Infection Using Microinjection |
| title_full_unstemmed | Pathogenicity of Treponema denticola Wild-Type and Mutant Strain Tested by an Active Mode of Periodontal Infection Using Microinjection |
| title_short | Pathogenicity of Treponema denticola Wild-Type and Mutant Strain Tested by an Active Mode of Periodontal Infection Using Microinjection |
| title_sort | pathogenicity of treponema denticola wild type and mutant strain tested by an active mode of periodontal infection using microinjection |
| url | http://dx.doi.org/10.1155/2012/549169 |
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